|本期目录/Table of Contents|

[1]谢佳佳 赛闹汪青 冉瑞兰 张牡丹 贾凌云 冯汉青**.镉胁迫对菜豆叶片抗氰呼吸、抗氧化及PSII光合特性的影响*[J].应用与环境生物学报,2019,25(02):1-8.[doi:10.19675/j.cnki.1006-687x.2018.07026]
 XIE Jiajia,SAINAO Wangqing,ZHANG Mudan,et al.Effects of Cadmium stress on Cyanide-resistant Respiratory, Antioxidation and PS II Photosynthetic Characteristic of Kidney Bean Leaves[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-8.[doi:10.19675/j.cnki.1006-687x.2018.07026]
点击复制

镉胁迫对菜豆叶片抗氰呼吸、抗氧化及PSII光合特性的影响*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-8
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Effects of Cadmium stress on Cyanide-resistant Respiratory, Antioxidation and PS II Photosynthetic Characteristic of Kidney Bean Leaves
文章编号:
201807026
作者:
谢佳佳 赛闹汪青 冉瑞兰 张牡丹 贾凌云 冯汉青**
西北师范大学 生命科学学院 兰州 730070
Author(s):
XIE Jiajia SAINAO Wangqing ZHANG Mudan RAN Ruilan JIA Lingyun FENG Hanqing*
College of Life Science, Northwest Normal University, Lanzhou 730070, China
关键词:
抗氰呼吸途径镉胁迫PS Ⅱ
Keywords:
C yanide-resistant respiratory pathway Cadmium stress PS II
DOI:
10.19675/j.cnki.1006-687x.2018.07026
摘要:
为探究镉胁迫下抗氰呼吸(交替途径)对植物体的保护作用和可能存在的作用机制,以菜豆幼苗为研究材料,探讨了在镉胁迫对菜豆叶片抗氰呼吸、抗氧化和光系统 Ⅱ (PS II) 光合特性的影响,以及抗氰呼吸对 PS II 的影响是否与其参与抗氧化调节作用有关。结果表明:随镉处理浓度的增加,菜豆叶片抗氰呼吸能力和抗氧化酶[过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶 (SOD)] 活性以及过氧化氢(H2O2)含量呈现先升高后降低趋势,而超氧阴离子自由基(O2·?)则逐渐显著上升;同时,菜豆幼苗叶片 Fv’/Fm’(光适应下 PS Ⅱ 最大光化学效率)、 Y( Ⅱ) (PS Ⅱ实际光化学效率) 和qP (光化学淬灭系数)、ETR (PSⅡ光合电子传递速率)下降,而叶片非光化学荧光猝灭系数(NPQ)则上升。非镉胁迫条件下,叶面分别喷施H2O2、O2·? 消除剂或抗氰呼吸抑制剂未对上述所测叶绿素荧光参数造成不良影响,且H2O2消除剂的施加使得 qP 和 ETR 显著提高。在镉胁迫下,叶面分别喷施H2O2或O2·? 消除剂有效缓解了镉导致的PS II 光化学活性的降低,而抗氰呼吸的抑制剂的施加则造成了镉胁造成的PS Ⅱ 光化学活性的进一步下降和H2O2和O2·? 含量的进一步提高,并导致了CAT、POD、APX抗氧化酶活性水平的降低。上述研究结果表明,在镉胁迫下抗氰呼吸可有效缓解镉胁迫导致的菜豆幼苗叶片活性氧的过量积累和抗氧化酶的活性的上升以及PS II 光化学活性的下降;且抗氰呼吸对PS II的保护作用可能与其调节抗氧化的功能有关。
Abstract:
In order to explore the protective effects and possible mechanisms of cyanide-resistant respiration (alternating pathway) on plants under cadmium stress . I n is paper, we investigated the effects of cadmium stress on cyanide-resistant respiration, antioxidation and photosynthetic characteristics of photosystem II (PS II) in kidney bean leaves, and w hether the effect of cyanide-resistant respiration on PS II is related to its involvement in antioxidant regulation. The results showed that the ability of cyanide-resistant breathing, the activity of antioxidant enzymes[including superoxide dismutase (SOD), catalase (CAT), POD(peroxidase), and ascorbate peroxidase(APX)] and the content hydrogen peroxide(H2O2) were increased first but then decreased with the increase of cadmium concentrations, while the superoxide anion radical (O 2·-) content was significantly increased with the increasing of cadmium concentrations. Simultaneously, the effective photochemical quantum yield of PS II photosynthetic [Y(II)], photochemical quenching coefficient (qP) and the rate of non-cyclic electrontransport through PS II (ETR) of leaves were decreased. While, the non-photochemical quenching (NPQ) of leaf was increased with the increasing of cadmium concentrations. Under condition without cadmium stress, spray with the exogenous eliminator of H2O2 and O 2·?, or inhibitor of cyanide-resistant respiratory on the leaf surface did not adversely affect above chlorophyll fluorescence parameters, but qP and ETR were significantly increased when the application of H2O2 eliminator . Under cadmium stress, treatment with the exogenous eliminator of H2O2 and O 2·? can effectively alleviated the decrease of photochemical activity of PS II, while treatment with the inhibitor of cyanide-resistant respiratory pathway caused further decrease of PS II photochemical efficiency and further increase of reactive oxygen species, and this treatment decreased the activities of antioxidant enzyme s. The above results showed that cyanide-resistant respiration can effectively alleviate the excessive accumulation of reactive oxygen species, the increase of antioxidant enzyme activity, and the decrease of PS II photochemical activity in the kidney bean seedling leaves caused by cadmium stress . And, the function of cyanide-resistant respiratory pathway in protect ing the PS II from Cd stress could be related to the ability of cyanide-resistant respiratory pathway to regulate the antioxidant capacity of plant.? ? ? ? ? ? ? ? ??

相似文献/References:

[1]王淑智,李利,张道勇,等.NaCl与Cd对小球藻光系统Ⅱ(PSⅡ)活性的影响[J].应用与环境生物学报,2011,17(06):839.[doi:10.3724/SP.J.1145.2011.00839]
 WANG Shuzhi,LI Li,ZHANG Daoyong,et al.Effects of NaCl and Cd on PhotosystemⅡ (PSⅡ) Activity of Chlorella pyrenoidosa[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):839.[doi:10.3724/SP.J.1145.2011.00839]
[2]陈颖,刘柿良,杨容孑,等.镉胁迫对龙葵生长、质膜ATP酶活性及氮磷钾吸收的影响[J].应用与环境生物学报,2015,21(01):121.[doi:10.3724/SP.J.1145.2014.07028]
 CHEN Ying,LIU Shiliang,YANG Rongjie,et al.Effects of cadmium on growth, plasma membrane ATPase activity, and absorption of N, P and K in Solanum nigrum L. seedlings[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):121.[doi:10.3724/SP.J.1145.2014.07028]

备注/Memo

备注/Memo:
收稿日期: 2018-07-19 接受日期 Accepted: 2018-10-15*国家自然科学基金(31560070,31260059)、甘肃省高等学校科研项目( 2015A- 007)、甘肃省重点研发计划项目、甘肃省高校基本科研业务费和西北师范大学青年创新团队资助 **通讯作者(E-mail: fenghanq@nwnu.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-14