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[1]叶 婷,刘亚男,王兴香,等.氯化汞对大鲵心脏和胰腺CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达的影响*[J].应用与环境生物学报,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.07025]
 YE Ting,LIU Yanan,WANG Xinxiang,et al.P450 2K1 ( CYP2K1 ) P450 2F3 ( CYP2F3 ) P450 2F5 ( CYP2F5 )P450 2C19 ( CYP2C19 )*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.07025]
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氯化汞对大鲵心脏和胰腺CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-9
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
P450 2K1 ( CYP2K1 ) P450 2F3 ( CYP2F3 ) P450 2F5 ( CYP2F5 )P450 2C19 ( CYP2C19 )*
文章编号:
201807025
作者:
叶 婷刘亚男王兴香曹 宇许抗抗李 灿**
贵阳学院生物与环境工程学院 贵州省山地珍稀动物与经济昆虫重点实验室,贵阳 550005
Author(s):
YE Ting LIU Yanan WANG Xinxiang CAO Yu XU Kangkang & LI Can**
College of Biology and Environmental Engineering, Guiyang University / Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, Guiyang 550005, China;
关键词:
大鲵氯化汞细胞色素P450 2K1 ( CYP2K1 ) 细胞色素P450 2F3 ( CYP2F3 ) 细胞色素P450 2F5 ( CYP2F5 )细胞色素P450 2C19 ( CYP2C19 )
Keywords:
Andrias davidianus mercuric chloride cytochrome P450 2K1 cytochrome P450 2F3 cytochrome P450 2F5 cytochrome P450 2C19
DOI:
10.19675/j.cnki.1006-687x.2018.07025
摘要:
探究氯化汞 ( HgCl2 ) 对大鲵心脏和胰腺细胞色素P450 2 ( CYP2 ) 亚家族CYP2K1 ) 、细胞色素P450 2F3 ( CYP2F3 )、细胞色素P450 2F5 ( CYP2F5 ) 和细胞色素P450 2C19 ( CYP2C19 ) 基因表达,为评价HgCl2对大鲵的生态毒理影响提供基础数据。本研究调查了幼龄大鲵暴露于HgCl2 ( 1、10和100 ng/L ) 24、48、72 h后,采用qRT-PCR试验分析HgCl2处理不同时间后CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19在心脏和胰腺中的表达变化模式。结果显示:HgCl2对大鲵暴露24 h后,大鲵心脏CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达水平相对于对照组均呈现上升变化趋势,即1 ng/L HgCl2显著诱导了大鲵心脏CYP2K1和CYP2C19基因表达水平 ( P<0.05 ) ,10 ng/L HgCl2显著诱导了大鲵心脏CYP2F3和CYP2F5基因表达水平 ( P<0.05 ) ,100 ng/L HgCl2均显著诱导了大鲵心脏CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达 ( P<0.05 ) ;大鲵胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达水平相对于对照组均呈现下降变化趋势,10和100 ng/L HgCl2均显著抑制了大鲵胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达 ( P<0.05 ) 。HgCl2对大鲵暴露48 h后,大鲵心脏CYP2F5基因表达水平相对于对照组均呈现显著下降变化趋势 ( P<0.05 ) ,并具有浓度梯度效应;大鲵胰腺CYP2F3基因表达水平受到显著诱导 ( P<0.05 ) 。HgCl2对大鲵暴露72 h后,与对照相比大鲵心脏和胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达均无显著性变化。本研究表明,随着HgCl2暴露时间延长,CYP2亚家族基因表达无显著变化,说明大鲵心脏和胰腺CYP2亚家族基因CYP2K1、CYP2F3、CYP2F5和CYP2C19表达对HgCl2刺激转变为慢性累计毒性效应。
Abstract:
The effects of mercuric chloride (HgCl2) on the expression of cytochrome P450 2K1 (CYP2K1), cytochrome P450 2F3 ( CYP2F3 ), cytochrome P450 2F5 ( CYP2F5 ) and cytochrome P450 2C19 ( CYP2C19 ) genes in cytochrome P450 2 ( CYP2 ) subfamily in the heart and pancreas of Chinese giant salamander Andrias davidianus were investigated, in order to provide basic data for evaluating the ecotoxicological effects of mercury chloride on the giant salamander. The juvenile stage giant salamander were continuously exposed to HgCl2 ( c, 1, 10, and 100 ng/L ) for 24, 48, 72 h respectively, and the effects of mercuric chloride on the expression of CYP2K1, CYP2F3, CYP2F5 and CYP2C19 in CYP2 subfamily in the heart and pancreas of giant salamander were investigated by real time quantitative RT-PCR method. After exposure of HgCl2 for 24 h, the expression levels of CYP2K1, CYP2F3, CYP2F5 and CYP2C19 genes in the heart of giant salamander were increased compared with the control group. For the heart of the giant salamander exposed of HgCl2 for 24 h, 1 ng/L HgCl2 significantly induced CYP2K1 and CYP2C19 gene expression levels ( P<0.05 ), 10 ng/L HgCl2 significantly induced CYP2F3 and CYP2F5 gene expression levels ( P<0.05 ), CYP2K1, CYP2F3, CYP2F5 and CYP2C19 expression levels were all significantly induced by 100 ng/L HgCl2 ( P<0.05 ). For the pancreas of the giant salamander, the expression levels of CYP2K1, CYP2F3, CYP2F5 and CYP2C19 genes in the pancreas of giant salamander showed a decreasing trend compared with the control group after exposed to HgCl2 for 24 h. Both 10 and 100 ng/L HgCl2 significantly inhibited the expression of CYP2K1, CYP2F3, CYP2F5 and CYP2C19 genes in the pancreas of the giant salamander exposed of HgCl2 for 24 h ( P<0.05 ). After exposure of HgCl2 for 48 h, the expression levels of CYP2F5 in the heart of giant salamander showed a significant decreasing trend with a dose-dependent effect ( P<0.05 ), and significant induction of CYP2F3 expression in the pancreas was showed ( P<0.05 ). After exposure of HgCl2 for 72 h, there were no significant changes in the expression of CYP2K1, CYP2F3, CYP2F5 and CYP2C19 genes in the heart and pancreas of the giant salamander compared with the control ( P>0.05 ). It was concluded that no significant change was showed on the expression of CYP2 subfamily gene with exposure time, which suggested the response of the CYP2 subfamily genes CYP2K1, CYP2F3, CYP2F5 and CYP2C19 in the heart and pancreas of Chinese giant salamander may be converted into a chronic cumulative toxic effect to stimulation of mercury chloride.? ? ? ? ? ??

备注/Memo

备注/Memo:
收稿日期: 2018-07-18 接受日期 Accepted: 2018-08-22*贵州省一流学科建设项目贵阳学院学科建设项目 ( 黔教科研发[2017]85 ) 、贵州省高层次创新人才“百”层次人才(黔科合人才(2016)4020号)、贵州省科学技术联合基金项目 ( 黔科合LH字[2015]7311 ) 、贵州省教育厅大鲵深加工技术开发产学研基地 ( 黔教合KY字[2014]231 ) 、贵州省大鲵可持续利用协同2011创新中心(黔教合协同创新字[2015]06)和贵阳市科技局贵阳学院专项资金GYU-KYZ【2018】02-11资助 **通讯作者(E-mail: lican790108@163.com)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-14