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[1]李 静 张金羽 张 琪 张潇月 李 玥 李成成 程雪芬赵 珂**.玉米根际无机磷溶解菌的筛选与促生特性*[J].应用与环境生物学报,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.06005]
 LI Jing,ZHANG Jinyu,ZHANG Qi,et al.Screening and plant growth promoting properties analysis of inorganic phosphate-solubilizing bacteria from maize rhizosphere*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.06005]
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玉米根际无机磷溶解菌的筛选与促生特性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-11
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Screening and plant growth promoting properties analysis of inorganic phosphate-solubilizing bacteria from maize rhizosphere*
文章编号:
201806005
作者:
李 静1 张金羽1 张 琪2 张潇月1 李 玥1 李成成1 程雪芬1赵 珂1**
1四川农业大学资源学院 成都 611130?
2 四川农业大学生命科学学院 雅安 625000
Author(s):
LI Jing1 ZHANG Jinyu1 ZHANG Qi2 ZHANG Xiaoyue1 LI Yue1 LI Chengcheng1 CHENG Xuefen1 & ZHAO Ke1**
1College of Resource and Environment, Sichuan Agricultural University, Chengdu 611130, China?
2College of Life sciences, Sichuan Agricultural University, Yaan, 625000, China
关键词:
玉米根际无机磷溶解菌筛选促生
Keywords:
maize rhizosphere inorganic phosphate-solubilizing bacteria sc reening plant growth promoting
DOI:
10.19675/j.cnki.1006-687x.2018.06005
摘要:
从玉米根际土中筛选对无机磷具有高效溶解作用和促生功能的菌株,为微生物肥料的开发提供菌株资源。使用溶磷圈法从玉米根际土中筛选溶磷菌株,采用钼锑抗比色法进一步测定菌株的溶磷能力。通过对菌株产IAA、产铁载体、固氮、产几丁质酶能力及抗菌活性进行检测,综合评价溶磷菌的促生能力,并通过盆栽实验探索单一菌株及复合菌系对玉米促生的潜能。结果表明:共筛选获得34株溶磷菌,其溶磷量在33.74-273.44 mg·L-1;34株菌中有10株菌(29.41%)同时具有3种及以上的促生功能,菌株PSM16、PSM27、PSM30同时具有4种促生功能;18株菌(52.94%)对不同供试病原菌表现出不同程度的抗性,菌株PSM12、PSM22、PSM25、PSM27、PSM34对5种目标病原菌同时具有抑菌作用;盆栽结果显示在土壤有效磷缺乏的条件下,经菌株PSM01、PSM12、PSM16及复合菌系处理后的玉米植株的株高、物质量、根系发达程度均显著提高(P<0.05),促生效果显著,且能提高土壤有效磷含量,复合菌系的效果更优。16S rRNA基因测序结果显示,PSM01、PSM12、PSM16分别属于Bacillus aryabhattai、Paenibacillus silvae、Pseudomonas moorei。综上所述,本研究为溶磷菌剂的开发提供了菌种资源,所构建的复合菌系具有较好的应用前景。图4 表4 参43
Abstract:
This study was designed to screening inorganic phosphate-solubilizing bacteria (PSB) possessed plant growth promoting capacity from maize rhizosphere, in order to provide strain resources for development and production of microbial fertilizer. Based on halo formation on screening medium to identify the phosphate solubilizing capacities of isolates. The Mo-Sb method was used to further analyze the phosphate solubilizing activity of str ains. The plant growth promoting properties of strains were evaluated by production of indole-3-acetic acid (IAA), siderophore, N2-fixation, production chitinase, and antimicrobial activity, respectively. The pot experiment was employed to evaluate the p otential plant growth promoting ability of pure culture and microbial consortia of PSB. The result showed that a total of 34 strains isolated from maize rhizosphere showing phosphate solubilizing activity. The amount of phosphate solubilization by isolates ranged from 33.74 mg·L -1 to 273.44 mg·L -1. 10 strains (29.41%) possessed at least three of plant growth promoting properties. Strain PSM16; PSM27 and PSM30 harbored plant growth promoting properties including production of IAA, siderophore, chitinase and nitrogen fixing ability. 18 strains (52.94%) showed antimicrobial activity, and strains PSM12, PSM22, PSM25, PSM27, PSM34 possessed antimicrobial activity against to 5 pathogens. The result of pot experiment exhibited all of PSM01, PSM12, PSM16 and PSB consortia could increase the maize biomass, promote the height and root growth significantly (P<0.05) under available phosphorus deficiency and improve soil available phosphorus, the best results were observed in PSB consortia. The 16S rRNA gene sequences a nalysis demonstrated the strain PSM01 、PSM12、PSM16 were belonged to Bacillus aryabhattai、Paenibacillus silvae、Pseudomonas moorei, respectively.This study provides strain resources for the development of biofertilizer and indicates that the PBS consortia is a good candidate to be applied as a biofertilizer to enhance soil fertility and promote plant growth.

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 Geng Ruishuang,Miao Shuhua,He Liming.MAIZE SEED PROTEIN MUTANTS RESISTANT TO LYSINE PLUS THREONINE[J].Chinese Journal of Applied & Environmental Biology,1995,1(02):1.
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备注/Memo

备注/Memo:
收稿日期: 2018-06-04 接受日期 Accepted: 2018-10-12?
*国家高技术研究发展计划(863计划,2013AA102802-05)资助?
**通讯作者(E-mail: zhaoke82@126.com)
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更新日期/Last Update: 2018-11-13