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[1]岳川,曹红利,王赞,等.茶树RING-finger型E3泛素连接酶基因CsSDIR的克隆与表达[J].应用与环境生物学报,2018,24(06):1375-1381.[doi:10.19675/j.cnki.1006-687x.2018.09010]
　YUE Chuan,et al..Cloning and expression of RING-finger E3 ubiquitin ligase CsSDIR1 gene in tea plant (Camellia sinensis)[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1375-1381.[doi:10.19675/j.cnki.1006-687x.2018.09010]
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茶树RING-finger型E3泛素连接酶基因CsSDIR的克隆与表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1375-1381
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Cloning and expression of RING-finger E3 ubiquitin ligase CsSDIR1 gene in tea plant (Camellia sinensis)
作者:
岳川;  曹红利;  王赞;  林宏政;  叶乃兴
福建农林大学园艺学院/茶学福建省高校重点实验室 福州 350002
Author(s):
YUE Chuan;  et al.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou 350002, China
关键词:
茶树; RING-finger E3泛素连接酶; CsSDIR1基因; 非生物胁迫; 表达分析
Keywords:
tea plant;  RING-finger E3 ubiquitin ligase;  CsSDIR1 gene;  abiotic stress;  expression analysis
分类号:
S571.1
DOI:
10.19675/j.cnki.1006-687x.2018.09010
摘要:
蛋白质泛素化修饰广泛参与植物的生长发育及逆境胁迫响应，其中RING-finger型E3泛素连接酶基因salt and drought induced ring finger1（SDIR1）在植物抗逆中具有重要的作用. 为了解茶树SDIR1（CsSDIR1）在抗逆应答中的作用机制，采用RT-PCR技术从茶树中克隆CsSDIR1的全长cDNA序列及启动子序列，对其生物信息学特征进行分析，并采用qRT-PCR技术检测该基因的组织表达特异性及在不同逆境胁迫下的表达模式. 结果显示，CsSDIR1基因的开放阅读框（ORF）长831 bp，编码276个氨基酸，蛋白质分子量（Mr）为30.085 × 103，理论等电点为6.54；氨基酸序列分析表明，CsSDIR1属于疏水性蛋白、定位在胞内膜上，与其他植物中的SDIR1相似性较高，在其N-端和C-端分别含有2个保守的跨膜结构域和C3H2C3 RING finger功能域；CsSDIR1与猕猴桃关系最近. CsSDIR1上游启动子含多个与干旱胁迫和盐胁迫响应相关的元件. 表达分析显示，CsSDIR1在茎中的表达量显著高于根、叶和花；ABA、干旱和高盐诱导其表达，低温抑制CsSDIR1的表达. 根据上述结果推测CsSDIR1基因可能参与了茶树的抗逆响应. （图6 参19）
Abstract:
Protein ubiquitination regulates many aspects of plant development and stress response. The RING-finger type E3 ubiquitin ligase SDIR1 (salt and drought induced ring finger 1) gene plays a key role in plant stress response. In this study, the full-length cDNA and the promoter sequences of CsSDIR1 were isolated from tea plants using the RT-PCR technology, and its bioinformatics characteristics were systematically analyzed. The expression patterns of CsSDIR1 in various tissues and in response to cold, drought, salt, and ABA treatments were also investigated by quantitative real-time RT-PCR (qRT-PCR). The sequence of CsSDIR1 contains a complete open reading frame of 831 bp, coding for a 276-long amino acid protein with a molecular weight of (Mr) 30.085 × 103 and a theoretical isoelectric point of 6.54. CsSDIR1 was predicted to be a hydrophobic protein localized on the intracellular membranes. The analysis of the amino acid sequence characteristics showed that CsSDIR1 contains two putative transmembrane domains at the N-terminus and a C3H2C3 RING-finger domain at the C-terminus; it shares high similarity with other plants’ SDIR1, and has the closest relationship to Actinidia sinensis. A cis-acting regulatory elements prediction showed that the CsSDIR1 promoter contains many cis-acting elements, especially drought and salt stress response elements. The qRT-PCR analysis indicated that the CsSDIR1 gene has a high expression level in stems, followed by roots, leaves, and flowers; the expression of the CsSDIR1 gene is up-regulated by ABA, salt, and drought treatments, whereas it is down-regulated in response to cold stress. These results demonstrated that the CsSDIR1 gene might be involved in the plant stress response of tea trees.

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更新日期/Last Update: 2018-12-25