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 ZHANG Mengjie,XU Liangliang,et al.Genetic diversity of Sichuan Basin populations of Microhyla fissipes inferred from mt-COI gene sequences[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1058-1064.[doi:10.19675/j.cnki.1006-687x.2018.01008]





Genetic diversity of Sichuan Basin populations of Microhyla fissipes inferred from mt-COI gene sequences
1南京师范大学生命科学学院 南京 210023 2中国科学院成都生物研究所 成都 610041
ZHANG Mengjie1 2 XU Liangliang1 2 ZHANG Jing2 Chang Qing1 WANG Bin2 ZHU Lifeng1** & JIANG Jianping2**
1 School of Life Sciences, Nanjing Normal University, Nanjing 210023, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Microhyla fissipes Sichuan Basin COI genetic population diversity population expansion
Q959.530.8 Genetic di
饰纹姬蛙(Microhyla fissipes)在中国广泛分布于秦岭以南地区,在四川盆地是常见的两栖动物之一,研究其种群遗传多样性可为当地两栖动物的保护与管理提供重要的遗传基础资料和理论依据. 采用线粒体COI基因为分子标记,探讨四川盆地饰纹姬蛙种群遗传多样性及其分化程度、种群历史动态. 共采集饰纹姬蛙28个种群354只,成功获得其COI基因618 bps片段序列. 分析结果发现17种单倍型,总体单倍型多样性(Hd)为0.166,核苷酸多样性(Pi)为 0.000 75,基因流(Nm)为1.60. 各种群间固定系数(Fst)和遗传分化系数(Gst)分别为0.270、0.140. 中性检验中,Tajima’s D = -2.380,P < 0.001;Fu’s Fs = -22.169,P < 0.02,均具有显著性差异;错配分布图呈泊松状的单峰. 通过计算得出种群扩张时间约在更新世晚期0.235 Ma BP. 上述结果表明,四川盆地饰纹姬蛙种群遗传多样性较低,种群间基因交流水平高,不同种群间遗传分化不明显,并且在历史上有过扩张的现象. (图5 表2 参47)
Microhyla fissipes is a frog that is widely distributed in China to the south of Qinling Mountains, especially in the Sichuan Basin, where it is considered to be one of the most common species. It is important that genetic data is collected on M. fissipes to provide a theoretical basis for the conservation and management of this and other local amphibian species. To study the genetic diversity, population differentiation, and history of M. fissipes in the Sichuan basin, the mitochondrial COI (mt-COI) gene was used as a molecular marker. A 618 bp fragment of the mt-COI gene was successfully sequenced in 354 individuals sampled from 28 populations in the Sichuan Basin. A total of 17 haplotypes were identified. The average haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.166 and 0.000 75, respectively. The estimated gene flow (Nm) among populations was 1.60. The fixation index (Fst) and genetic differentiation coefficient (Gst) among all groups were 0.270 and 0.140, respectively. The Neutral test showed significant differences (Tajima’s D value = -2.380, P < 0.001; Fu’s Fs value = -22.169, P < 0.02), and the mismatch distribution maps showed a single peak corresponding to a Poisson distribution, which indicated that M. fissipes has undergone a population expansion recently. The population expansion time was calculated to be about 0.235 Ma BP (millions of years before present), in the late Pleistocene. These results indicated that the population genetic diversity of M. fissipes was relatively low, while the level of genetic exchange among populations was contrarily found to be high. In this case, no obvious differentiation among the various populations in the Sichuan Basin was observed. Actually, the population expansion we inferred for M. fissipes had existed in history.


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 LI Yulong,WANG Xungang,et al.Morphological variation of Microhyla fissipes on Hainan Island[J].Chinese Journal of Applied & Environmental Biology,2017,23(05):1135.[doi:10.3724/SP.J.1145.2017.03021]

更新日期/Last Update: 2018-10-25