|本期目录/Table of Contents|

[1]任海伟,刘菲菲,李梦玉,等.玉米秸秆和白菜废弃物在不同贮存条件下的微生物群落[J].应用与环境生物学报,2018,24(02):281-291.[doi:10.19675/j.cnki.1006-687x.2017.05035]
　REN Haiwei,LIU Feifei,LI Mengyu,et al.Microbial community of maize straw and cabbage waste under different storage conditions[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):281-291.[doi:10.19675/j.cnki.1006-687x.2017.05035]
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玉米秸秆和白菜废弃物在不同贮存条件下的微生物群落()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
281-291
栏目:
研究论文
出版日期:
2018-04-25

文章信息/Info

Title:
Microbial community of maize straw and cabbage waste under different storage conditions
作者:
任海伟; 刘菲菲; 李梦玉; 王蕾; 李金平; 李志忠; 李东; 黄娟娟; 孙文斌
1兰州理工大学西部能源与环境研究中心 兰州 7300502兰州理工大学生命科学与工程学院 兰州 7300503甘肃省生物质能与太阳能互补供能系统重点实验室 兰州 730050 4中国科学院环境与应用微生物重点实验室（成都生物研究所） 成都 610041
Author(s):
REN Haiwei;  LIU Feifei;  LI Mengyu;  WANG Lei;  LI Jinping;  LI Zhizhong;  LI Dong;  HUANG Juanjuan;  SUN Wenbin
1 Western Energy & Environment Research Center, Lanzhou University of Technology, Lanzhou 730050, China 2 School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China3 Key Laboratory of Complementary Energy System of Biomass and Solar Energy in Gansu Province, Lanzhou 730050, China4 Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
高通量测序; 玉米秸秆; 白菜废弃物; 微生物群落结构; OTU数; Alpha多样性
Keywords:
Illumina Miseq;  maize straw;  cabbage waste;  microbial community;  OTU;  Alpha diversity.
分类号:
Q938.1
DOI:
10.19675/j.cnki.1006-687x.2017.05035
摘要:
采用Illumina Miseq高通量技术分析不同温度和贮存方式下玉米秸杆（IO）和白菜废弃物（IA）贮存30 d时的微生物群落. 设置低温（O）、中温（R）和高温（H）3种温度；每种温度条件均设有秸杆单贮（O）、白菜单贮（A）和二者混贮（X）3个处理组. 结果显示，IO原料附着细菌主要包括变形菌门（Proteobacteria）（65.26%）和厚壁菌门（Firmicutes）（33.78%），IA主要包括Proteobacteria（80.23%）和拟杆菌门（Bacteroidetes）（18.57%）. 贮存30 d后在属水平上，IO主要包括肠杆菌（Enterobacter）（47.11%）、肉食杆菌（Carnobacterium）（27.71%）和泛菌（Pantoea）（10.14%）等；IA主要包括假单孢菌（Pseudomonas）（48.40%）、Pantoea（17.10%）和黄杆菌（Flavobacterium）（16.26%）等；IA中几乎不含乳酸菌，IO中乳酸菌丰度约28.71%. IO组低、高温单贮时主要包括Enterobacter（21.76%和35.87%）、Carnobacterium（40.42%和27.29%）和Pseudomonas（18%和26.99%），中温单贮时主要包括Enterobacter（66.72%）；IA中、高温单贮时主要包括乳杆菌（Lactobacillus）（80.07%和74.63%），低温单贮时主要包括Lactobacillus（28.43%）、耶尔森氏鼠疫杆菌（Yersinia）（19.50%）和Enterobacter（17.13%）；二者低、中温混贮时主要包括Lactobacillus（52.03%和53.52%）、Enterobacter（5.98%和11.65%）和Carnobacterium（12.98%和10.65%），高温混贮时主要包括Enterobacter（70.57%）. 综上表明，高通量测序技术全面反映了IO和IA在不同贮存条件下细菌群落的组成及丰度信息，白菜中高温单独贮存、秸秆/白菜中低温混合贮存时乳酸菌占优势. （图9 表3 参37）
Abstract:
The microbial community of maize straw (IO) and cabbage waste (IA) under different storage temperatures and storage patterns for 30 days was analyzed by Illumina Miseq high-throughput sequencing technologies. Different temperature conditions, including low temperature (O), medium temperature (R), and high temperature (H) conditions, were set up with three groups [sole-storage of maize straw (O), sole-storage of cabbage waste (A), and mixed storage of IO and IA (X)]. The results showed that the dominant bacteria attached to the IO at the phylum level were Proteobacteria (65.26%) and Firmicutes (33.78%), whereas the dominant bacteria attached to the IA were Proteobacteria (80.23%) and Bacteroidetes (18.57%). The dominant bacteria in different treatment groups after storage for 30 days were Proteobacteria and Firmicutes. The IO mainly included Enterobacter (47.11%), Carnobacterium (27.71%), and Pantoea (10.14%) at the genus level. The IA mainly included Pseudomonas (48.40%), Pantoea (17.10%), and Flavobacterium (16.26%). The IA contained almost no Lactobacillus, whereas the abundance of Lactobacillus in the IO was about 28.71%. After sole-storage of IO, the low and high temperature groups mainly included Enterobacter (21.76% and 35.87%), Carnobacterium (40.42% and 27.29%), and Pseudomonas (18.00% and 26.99%). The medium temperature group mainly included Enterobacter (66.72%) and Pantoea (10.40%). After sole-storage of IA, the medium and high temperature groups mainly included Enterobacter (8.62% and 11.24%) and Lactobacillus (80.07% and 74.63%), and the low temperature group mainly included Enterobacter (17.13%), Lactobacillus (28.43%), and Yersinia (19.50%). After mixed storage for 30 days, the low and medium temperature groups mainly included Lactobacillus (52.03% and 53.52%), Enterobacter (5.98% and 11.65%), and Carnobacterium (12.98% and 10.65%). The high temperature group mainly included Enterobacter (70.57%). In conclusion, the microbial community and abundance information of IO and IA under different storage conditions were fully reflected by the Illumina Miseq high-throughput sequencing technology. The dominant bacteria in the medium and high temperature groups of sole-storage of IA, and the medium and low temperature groups of mixed storage of IA and IO was the lactic acid bacteria.

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更新日期/Last Update: 2018-04-25