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[1]刘娜,陆震鸣,王丽娟,等.克隆文库分析镇江香醋发酵过程中乙偶姻合成基因的多样性[J].应用与环境生物学报,2017,23(01):134-139.[doi:10.3724/SP.J.1145.2016.01052]
 LIU Na,LU Zhenming,WANG Lijuan,et al.Clone library analysis of enzyme genes in metabolic pathway for acetoin production in the fermentation of Zhenjiang aromatic vinegar[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):134-139.[doi:10.3724/SP.J.1145.2016.01052]
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克隆文库分析镇江香醋发酵过程中乙偶姻合成基因的多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年01期
页码:
134-139
栏目:
研究论文
出版日期:
2017-02-25

文章信息/Info

Title:
Clone library analysis of enzyme genes in metabolic pathway for acetoin production in the fermentation of Zhenjiang aromatic vinegar
作者:
刘娜陆震鸣王丽娟余永健李国权史劲松许正宏
1江南大学药学院,工业生物技术教育部重点实验室 无锡 214122 2国家固态酿造工程技术研究中心 泸州 646000 3中国科学院天津工业生物技术研究所,天津市工业生物系统与过程工程重点实验室 天津 300308 4江苏恒顺醋业股份有限公司 镇江 212043
Author(s):
LIU Na1 LU Zhenming1 WANG Lijuan1 YU Yongjian4 LI Guoquan4 SHI Jinsong1 2 & XU Zhenghong1 2 3**
1School of Pharmaceutical Science, Key Laboratory of Industrial Microbiology of Ministry of Education, Jiangnan University, Wuxi 214122, China 2National Engineering Research Center of Solid-State Brewing, Luzhou 646000, China 3Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China 4Jiangsu Hengshun Vinegar Industry Co., Ltd., Zhenjiang 212043, China
关键词:
镇江香醋乙偶姻基因多样性克隆文库微生物群落乙酰乳酸合成酶乙酰乳酸脱羧酶
Keywords:
Zhenjiang aromatic vinegar acetoin gene diversity clone library microbial community acetolactate synthase acetolactate decarboxylase
分类号:
TS264.22 : Q785
DOI:
10.3724/SP.J.1145.2016.01052
摘要:
乙偶姻是传统发酵食醋重要的风味物质,也是食醋中活性物质川芎嗪形成的重要前体. 采用克隆文库分析方法对镇江香醋醋醅中乙偶姻合成途径的两个关键酶(乙酰乳酸合成酶ALS,乙酰乳酸脱羧酶ALDC)的基因多样性进行分析. 结果表明,ALS基因克隆文库中相对丰度最大为Lactobacillus buchneri(65.7%),其次为Acetobacter pasteurianus(21.3%)、L. reuteri(10.2%)、L. casei(1.9%)、L. brevis(0.9%);而ALDC基因克隆文库中A. pasteurianus(91.2%)占绝对优势,且呈现丰富的种内基因多样性,另外还有少量L. fermentum(8.8%). 因此,Acetobacter和Lactobacillus可能是醋醅微生物群落中利用丙酮酸合成乙偶姻的主要参与者;本研究可为解析多菌种醋酸发酵过程中乙偶姻微生物合成机理奠定研究基础,同时为其他微生物群落功能的研究提供参考. (图5 表1 参28)
Abstract:
This research aimed to study the gene diversity of two key enzymes in the acetoin pathway in the vinegar Pei and to lay foundation for the functional microbial community reconstruction. Degenerate primers were designed, validated and used for the amplification of gene coding for acetolactate synthase (ALS) and acetolactate decarboxylase (ALDC) in vinegar Pei. Then two gene clone libraries were established respectively and analyzed by rarefaction curve, cluster analysis and phylogenetic analysis. Two pairs of degenerate primers were validated to be of high specificity and used for the clone libraries construction. Sequences in ALS clone library were clustered to 6 Operational Taxonomic Units (OTUs) and the most abundant species was proved to be Lactobacillus buchneri (65.74%). Sequences in ALDC clone library were clustered to 16 OTUs and the most abundant species was Acetobacter pasteurianus (91.23%). The research found that Lactobacillus and Acetobacter might be the main functional genera for the production of ALS and ALDC in the vinegar Pei.

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更新日期/Last Update: 2017-02-25