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[1]王艳发,魏士平,崔鸿鹏,等.祁连山冻土区土壤活动层与冻土层中甲烷代谢微生物群落结构特征[J].应用与环境生物学报,2016,22(04):592-598.[doi:10.3724/SP.J.1145.2016.01051]
 WANG Yanfa,WEI Shiping**,et al.Methane metabolic microbial community structure in the active layer and the permafrost layer of the Qilian permafrost, China*[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):592-598.[doi:10.3724/SP.J.1145.2016.01051]
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祁连山冻土区土壤活动层与冻土层中甲烷代谢微生物群落结构特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年04期
页码:
592-598
栏目:
高寒生态系统的结构、功能与动态专栏
出版日期:
2016-08-25

文章信息/Info

Title:
Methane metabolic microbial community structure in the active layer and the permafrost layer of the Qilian permafrost, China*
作者:
王艳发魏士平崔鸿鹏苏新胡非祝有海卢振权刘晖张帅庞守吉
1中国地质大学(北京)海洋学院 北京 1000832地质过程与矿产资源国家重点实验室 北京 1000833中国地质调查局油气资源调查中心 北京 100029
Author(s):
WANG Yanfa1 2 WEI Shiping1** CUI Hongpeng1 2 SU Xin1 2** HU Fei1 2 ZHU Youhai3 LU Zhenquan3 LIU Hui3 ZHANG Shuai3 & PANG Shouji3
1School of Ocean Sciences, China University of Geosciences, Beijing 100083, China2State Key Laboratory of Geological Processes and Mineral Resources, Beijing 100083, China3Oil and Gas Survey, China Geological Survey, Beijing 100029, China
关键词:
祁连山冻土微生物群落结构产甲烷菌甲烷氧化菌
Keywords:
Qilian Mountain permafrost microbial community structure methanogens methanotrophs
分类号:
S154.3 : X172
DOI:
10.3724/SP.J.1145.2016.01051
摘要:
冻土土壤中的甲烷代谢微生物可氧化或产生甲烷,影响着甲烷所参与的碳循环过程,对于全球温室气体的释放和调节具有重要的作用. 对祁连山冻土区土壤活动层与冻土层中的甲烷代谢微生物产甲烷菌(Methanogens)和甲烷氧化菌(Methanotrophs)的群落结构组成进行研究. 通过对产甲烷菌的mcrA基因和甲烷氧化菌的pmoA基因进行PCR扩增,分别构建其基因克隆文库,并通过序列同源比对进行系统发育分析和多样性分析. 结果显示:冻土土壤活动层中的产甲烷菌包括Rice cluster I、Methanosarcinaceae、Methanomicrobiales、Methanosaetaceae、Methanobacteriaceae五种类型,而在土壤冻土层则包括了Rice cluster I、Methanosarcinaceae、Methanobacteriaceae三种类型. 土壤活动层的甲烷氧化菌由隶属于α-Proteobacteria(Type II)的Methylocystis和隶属于γ-Proteobacteria(Type I)的Methylobacter两种类型群体组成,而土壤冻土层中则只包括了Methylocystis这一种类型. 由此可见,冻土土壤活动层与冻土层中的甲烷代谢微生物群落结构存在一定的差异. (图5 表3 参39)
Abstract:
Methane metabolic microbial community in the permafrost soil can produce or oxidize methane, which affects the processes of permafrost methane-cycling, therefore has an important role in the global greenhouse release and regulation. At present, the research of methane metabolic microbial community structure in the soil of permafrost has received extensive attention. In order to understand the distribution and diversity of methane metabolic microbial in the permafrost, we studied the methanogens and methanotrophs community compositions in the active layer and the permafrost layer of Qilian Mountain, China. The molecular biology methods were used to amplify the methanogens mcrA genes and the methanotrophs pmoA genes, then their gene clone libraries constructed. Phylogenetic analysis of the sequences based on sequence similarity was carried out and the community diversity was calculated. The retrieved methanogens mcrA genes sequences were classified into five lineages. The predominant groups of the active layer belonged to Rice cluster I, Methanosarcinaceae, Methanomicrobiales, Methanosaetaceae and Methanobacteriaceae, and those in the permafrost layer included Rice cluster I, Methanosarcinaceae and Methanobacteriaceae. All the methanotrophs pmoA genes sequences were affiliated to the same phylum Proteobacteria. In the active layer, Proteobacteria was classified into two lineage including Methylocystis (α-Proteobacteria, type II) and Methylobacter (γ-Proteobacteria, type I). In the permafrost layer, Methylocystis was the predominant lineage. The results indicated significant difference in methane metabolic microbial composition and distribution between the active layer and the permafrost layer of Qilian Mountain.

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更新日期/Last Update: 2016-08-25