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[1]唐 标 肖新云 刘又嘉 姚天振 尹抗抗** 谭周进.降脂理肝汤对高脂饮食诱导的非酒精性脂肪肝大鼠肠道微生物及酶活性的影响[J].应用与环境生物学报,2016,22(03):442-445.[doi:10.3724/SP.J.1145.2015.11001]
 TANG Biao,XIAO Xinyun,LIU Youjia,et al.Effects of Jiangzhiligan Decoction on intestinal microbiota and enzyme activities of rats with non-alcoholic fatty liver disease induced by high-fat diet*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):442-445.[doi:10.3724/SP.J.1145.2015.11001]
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降脂理肝汤对高脂饮食诱导的非酒精性脂肪肝大鼠肠道微生物及酶活性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
442-445
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
Effects of Jiangzhiligan Decoction on intestinal microbiota and enzyme activities of rats with non-alcoholic fatty liver disease induced by high-fat diet*
作者:
唐 标 肖新云 刘又嘉 姚天振 尹抗抗** 谭周进
湖南中医药大学 长沙 410208
Author(s):
TANG Biao XIAO Xinyun LIU Youjia YAO Tianzhen YIN Kangkang** & TAN Zhoujin**
Hunan University of Chinese Medicine, Changsha 410208, China
关键词:
降脂理肝汤非酒精性脂肪肝肠道微生物肠道酶
Keywords:
Jiangzhiligan Decoction nonalcoholic fatty liver disease intestinal microorganisms intestinal enzyme
分类号:
R285.5
DOI:
10.3724/SP.J.1145.2015.11001
摘要:
为了解降脂理肝汤对非酒精性脂肪肝病(NAFLD)大鼠肠道微生物及其酶活性的影响,建立高脂饮食诱导的NAFLD大鼠模型,以降脂理肝汤治疗4周后,分析比较体重、肝重、肝指数、肠道菌群数和酶活. 结果显示,模型组大鼠体重、肝重和肝指数明显高于正常组(P < 0.01),肠道细菌总数、大肠杆菌和乳酸菌数量明显多于正常组(P < 0.05),肠道木聚糖酶、淀粉酶和蛋白酶活性高于正常组(P < 0.05);降脂理肝汤显著降低NAFLD大鼠体重、肝重和肝指数(P < 0.05),并且能抑制肠道细菌的生长,抑制大肠杆菌和乳酸菌的生长(P < 0.05),使细菌总数和乳酸菌数量恢复至正常组水平(P > 0.05). 此外,降脂理肝汤还能抑制NAFLD大鼠肠道木聚糖酶、淀粉酶和蛋白酶活性(P < 0.05),使其恢复到正常组水平(P > 0.05). 综上表明降脂理肝汤能调整NAFLD大鼠肠道菌群种群,改善高脂饮食诱导的NAFLD,结果可为该方的临床应用提供科学依据. (图3 表2 参18)
Abstract:
This research aimed to provide scientific basis for clinical use of Jiangzhiligan Decoction (JZLGT) by exploring its effect on intestinal microbiota and enzyme activities of rats with non-alcoholic fatty liver disease (NAFLD). The NAFLD model was constructed with high-fat diet in rats, and then the modeled rats were treated with JZLGT for four weeks. The body weight, liver weight, liver index, intestinal microbiota and enzyme activities were determined. The results showed higher body weight, liver weight and liver index in the model group than in the normal group (P < 0.01); the quantities of intestinal bacteria, Escherichia coli and Lactobacillus, the activities of xylanase, protease and amylase were also higher in the model group than the normal group (P < 0.05). After treatment, rats in the JZLGT group had significantly lower body weight, liver weight and liver index than those of the model group (P < 0.05); the quantities of intestinal bacteria, Escherichia coli and Lactobacillus, as well as the activities of xylanase, protease and amylase of the JZLGT group were also significantly lower (P < 0.05). Comparison with the normal group showed that the quantities of intestinal bacteria and Lactobacillus, the activities of xylanase, protease and amylase in the JZLGT group recovered to the normal level (P > 0.05). The results suggested that JZLGT can inhibit intestinal bacterial overgrowth and relieve NAFLD induced by high fat diet.

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更新日期/Last Update: 2016-06-25