|本期目录/Table of Contents|

[1]刘 敏 王长庭** 字洪标 胡 雷 杨希智 杨有芳.火烧干扰下高寒草甸土壤微生物群落功能多样性变化特征[J].应用与环境生物学报,2016,22(02):263-270.[doi:10.3724/SP.J.1145.2015.08019]
　LIU Min,WANG Changting**,ZI Hongbiao,et al.Effects of fire disturbance on the functional diversity of soil microbialcommunity in alpine meadows*[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):263-270.[doi:10.3724/SP.J.1145.2015.08019]
点击复制

火烧干扰下高寒草甸土壤微生物群落功能多样性变化特征()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年02期
页码:
263-270
栏目:
研究论文
出版日期:
2016-04-25

文章信息/Info

Title:
Effects of fire disturbance on the functional diversity of soil microbialcommunity in alpine meadows*
作者:
刘 敏1 王长庭1** 字洪标1 胡 雷1 杨希智2 杨有芳1
1西南民族大学生命科学与技术学院 成都 6100412仪陇县农牧业局饲料站 仪陇 637600
Author(s):
LIU Min1;  WANG Changting1**;  ZI Hongbiao1;  HU Lei1;  YANG Xizhi2 & YANG Youfang1
1College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China2Feed Station of Yilong Agricultural Husbandry Bureau, Yilong 637600, China
关键词:
火烧干扰; 土壤微生物群落; 高寒草甸; Biolog-ECO板; 碳源利用
Keywords:
fire disturbane;  soil microbial community;  alpine meadow fire disturbance;  Biolog-ECO;  carbon source utilization
分类号:
S812.2 : S154.36
DOI:
10.3724/SP.J.1145.2015.08019
摘要:
为揭示火烧干扰对高寒草甸土壤微生物群落的影响，应用Biolog法研究火烧恢复初期土壤微生物群落、碳源利用类型和功能多样性变化特征及其与土壤理化性质的相关性. 结果表明：1）恢复初期火烧样地土壤微生物群落代谢活性显著高于对照样地（P < 0.05），火烧土壤微生物平均颜色变化率（1.20）高于对照样地（1.07）；2）火烧干扰后高寒草甸土壤微生物群落Shannon-Wiener指数、Pielou指数、McIntosh指数和和碳源利用数分别显著增加了0.037、0.203、1.234和2.340（P < 0.05）；3）主成分分析表明，火烧使微生物代谢功能特征发生变化，碳水化合物是土壤微生物利用的主要碳源类型；4）相关性分析表明，土壤pH、有机质（SOM）、全磷（TP）与微生物功能多样性指数呈显著相关（P< 0.05），土壤碱解氮（AN）、速效磷（AP）、速效钾（AK）、全氮（TN）和根土比与碳水化合物类碳源呈正显著相关（P < 0.05）. 综上，火烧恢复初期，土壤有效养分提高，微环境得以改善，土壤微生物代谢活性、碳源利用能力和数量明显提高. （图4 表2 参52）
Abstract:
This study aimed to explore the effects of fire disturbance on the soil microbial community in an alpine meadow.Biolog-ECO was used to study the changes of soil microbial community composition and functional diversity of carbon sourceutilization in the early stage of fire recovery. The results showed that in the early stage of fire recovery, metabolic activity ofsoil microbial community was higher in the fire disturbed plot than the control plot, the average well color development rate(1.20) of the burned soil was higher than that of the control sample (1.07). The carbon source utilization, Shannon-Wiener,Pielou and McIntosh index of soil microbial community increased significantly by 0.037, 0.203, 1.234 and 2.340 respectively.Principal component analysis (PCA) indicated that fire disturbance changed microbial metabolic function and carbohydratewas the major carbon source type for the soil microbial community. Correlation analysis showed that soil pH, organic matter(SOM) and total phosphorus (TP) had significant correlation with microbial functional diversity indexes (P < 0.05), and thesoil available nitrogen (AN), available phosphorus (AP), available potassium (AK), total nitrogen (TN) and root soil ratio hadsignificantl positive correlation with carbohydrate carbon source (P < 0.05). In summary, in the early stage of fire recovery, soilavailable nutrients increase, resulting in significantly improved microenvironment, soil microbial metabolic activity and carbonsource utilization.

参考文献/References:

1 周道玮, 张喜军, 张宏. 草地火生态学研究进展[M]. 长春: 吉林科学技术出版社, 1995.2 张敏, 马鸿伟. 林火对土壤细根系生物量影响的研究[J]. 林业科技,2003, 28 (2): 30-33 [Zhang M, Ma HW. Effect of forest fire on fine root insoil [J]. For Sci Technol, 2003, 28 (2): 30-33]3 Brinkmann WLF, Nascimento JD. Effect of slash and burn agriculture onplant nutrients in the Tertiary region of central Amazonia [J]. Turrialba,1973, 3 (1): 55-614 Johnson AF, Abrahamson WG. A note on fire responses of speciesin rosemary scrubs on the southern Lake Wales Ridge0 [J]. FloridaScientist, 1990, 53: 138-1435 高湋. 火在生态系统中的作用[J]. 生态学杂志, 1992, 11 (1): 44-47 [GaoW. Fire and its roles in ecosystem [J]. Chin J Ecol, 1992, 11 (1): 44-47]6 Belsky AJ. Effects of grazing, competition, disturbance and fire onspecies composition and diversity in grassland communities [J]. J VegSci, 1992, 3 (2): 187-2007 Pereira P, Cerda A, Jordan A, Bolutiened V, Pranskeviciusd M, VioletaBolutiened, Ubedae X, Mataix-soleraf J. Spatio-temporal vegetationrecuperation after a grassland fire in Lithuania [J]. Procedia Environ Sci,2013, 19: 856-8648 Docherty KM, Balser TC, Bohannan BJM, Gutknecht JLM. Soilmicrobial responses to fire and interacting global change factors in aCalifornia annual grassland [J]. Biogeochemistry, 2012, 109 (1-3): 63-839 Palmquist KA, Peet RK, Weakley AS. Changes in plant species richnessfollowing reduced fire frequency and drought in one of the most speciesrichsavannas in North America [J]. J Veg Sci, 2014, 25 (6): 1426-143710 宋启亮, 董希斌, 李勇, 刘继明. 采伐干扰和火烧对大兴安岭森林土壤化学性质的影响[J]. 森林工程, 2010, 26 (5): 4-7 [Song QL, DongXB, Li Y, Liu Y, Liu JM. Impacts of burning and logging disturbance onsoil chemical properties of forest in Daxinganling Mountain Region [J].For Eng, 2010, 26 (5): 4-7]11 王明玉, 舒立福, 王景升, 田晓瑞, 李红. 西藏东南部森林可燃物特点及气候变化对森林火灾的影响[J]. 火灾科学, 2007, 16 (1): 15-20[Wang MY, Shu LF, Wang JS, Tian XR, Li H. Forest fuel characteristicsand the impact of climate change on forest fires in Southeast Tibet [J].Fire Saf Sci, 2007, 16 (1): 15-20]12 周道玮, 张宝田, 张宏一, 陆静梅. 松嫩草原不同时间火烧后群落特征的变化[J]. 应用生态学报, 1996, 7 (1): 39-43 [Zhou DW, Zhang BTZhang HY, Lu JM. Community variation on Songnen grassland afterdifferent burning time [J]. Chin J Appl Ecol, 1996, 7 (1): 39-43]13 林燕, 白永飞. 内蒙古典型草原小叶锦鸡儿灌丛地上净初级生产力和种群结构对火烧的响应[J]. 草业学报, 2010, 19 (5): 170 [Lin Y, BaiYF. Responses of aboveground net primary production and populationstructure of Caragana microphylla to prescribed burning in typicalsteppe of Inner Mongolia [J]. Acta Pratacult Sin, 2010, 19 (5): 170]14 向泽宇, 陈瑞芳, 蒋忠荣, 呷绒仁青, 杨俊莲, 王长庭, 胡雷. 川西北高寒草甸对火烧干扰的短期响应[J]. 草业科学, 2014, 31 (11): 2034-2041 [Xiang ZY, Chen RF, Jiang ZR, GaRong RQ, Yang JL, Wang CT,Hu L. The short-term responses of alpine meadow to fire disturbance inNorthwest Sichuan [J]. Pratacult Sci, 2014, 31 (11): 2034-2041]15 Knicker H. How does fire affect the nature and stability of soil organicnitrogen and carbon? a review [J]. Biogeochemistry, 2007, 85 (1): 91-11816 孙明学, 贾炜玮, 吴瑶. 大兴安岭北部地区林火对土壤化学性质的影响[J]. 东北林业大学学报, 2009, 37 (5): 33-35 [Sun XM, Jia WW, WuY. Effect of forest fire on soil chemical properties in northern Daxing’anmountains [J]. J Northeast For Univ, 2009, 37 (5): 33-35]17 楚旭, 邸雪颖, 杨光. 林火对兴安落叶松根生物量及碳氮养分浓度的影响[J]. 北京林业大学学报, 2013, 35 (2): 10-16 [Chu X, Di XY,Yang G. Impacts of forest fire on root biomass, carbon and nitrogenconcentration of Larix gmelinii [J]. J Beijing For Univ, 2013, 35 (2): 10-16]18 贺郝钰, 苏洁琼, 黄磊, 贾荣亮, 李新荣. 火因子对荒漠化草原草本层片植物群落组成的影响[J]. 生态学报, 2011, 31 (2): 364-370 [He HY,Su JJ, Huang L, Jia RL, Li XR. Effects of fire on the structure of herbagesynusia vegetation in desertified steppe, North China [J]. Acta Ecol Sin,2011, 31 (2): 364-370]19 贺郝钰, 李新荣, 苏洁琼. 火烧对荒漠化草原土壤性质的影响[J]. 生态学杂志, 2013, 32 (12): 3312-3317 [He HY, Li XR, Su JQ. Effects offire on soil properties in desertified steppe of Northwest China [J]. ChinJ Ecol, 2013, 32 (12): 3312-3317]20 张敏, 胡海清. 林火对土壤微生物的影响[J]. 东北林业大学学报,2002, 30 (4): 44-46 [Zhang M, Hu HQ. The effects of forest fire onmicroorganism in soil [J]. J Northeast For Univ, 2002, 30 (4): 44-46]21 Paul EA, Clark FE. Soil microbiology and biochemistry [M]. 2nd ed.San Diego: Academic Press, 199622 Zhang N, Wan SQ, Li LH, Bi J, Zhao MM, Ma KP. Impacts of urea Naddition on soil microbial community in a semi-arid temperate steppe innorthern China [J]. Plant Soil, 2008, 311 (1-2): 19-2823 刘定辉, 舒丽, 陈强, 陈尚洪, 陈红琳, 朱钟麟. 秸秆还田少免耕对冲积土微生物多样性及微生物碳氮的影响[J]. 应用与环境生物学报, 2011, 2: 158-161 [Liu DH, Shu L, Chen Q, Chen SH, Chen HL, ZhuZL. Effects of straw mulching and little-or zero-tillage on microbialdiversity and biomass C and N of alluvial soil in Chengdu Plain, China[J]. Chin J Appl Ecol, 2011, 2: 158-161]24 Zelles L. Fatty acid patterns of phospholipids and lipopolysaccharides inthe characterisation of microbial communities in soil: a review [J]. BiolFertil Soils, 1999, 29 (2): 111-12925 郑华, 欧阳志云, 方治国, 赵同谦. BIOLOG在土壤微生物群落功能多样性研究中的应用[J]. 土壤学报, 2004, 41 (3): 456-461 [Zheng H,Ouyang ZY, Fang ZG, Zhao TQ. Application of BIOLOG to study onsoil microbial community functional diversity [J]. Acta Pedol Sin, 2004,41 (3): 456-461]26 时鹏, 高强, 王淑平, 张研. 玉米连作及其施肥对土壤微生物群落功能多样性的影响[J]. 生态学报, 2010, 30 (22): 6173-6182 [Shi P,Gao Q, Wang SP, Zhang Y. Effects of continuous cropping of corn andfertilization on soil microbial community functional diversity [J]. ActaEcol Sin, 2010, 30 (22): 6173-6182]27 王乾, 朱单, 吴宁, 罗鹏. 四川道孚县芒苞草生境的植物群落结构和土壤元素含量[J]. 应用与环境生物学报, 2009, 15 (1): 1-7 [WangQ, Zhu D, Wu N, Luo P. Plant community structure and soil elementcontent in Achanthoclamys bracteata habitats in Daofu County [J]. ChinJ Appl Ecol, 2009, 15 (1): 1-7]28 李娜. 川西亚高山森林植被生物量及碳储量遥感估算研究[D]. 四川: 四川农业大学, 2008: 12-13 [Li N. Western sichuan subalpine forestvegetation biomass and carbon remote sensing estimation research [D].Sichuan: Sichuan Agricultural University, 2008: 12-13]29 Garland JL, Mills AL. Classif ication and character ization ofheterotrophic microbial communities based on the basis of patternsof community-level sole carbon-source utilization [J]. Appl EnvironMicrobiol, 1991, 57 (8): 2351-235930 向泽宇, 张莉, 张全发, 刘伟 王根绪 王长庭 胡雷. 青海不同林分类型土壤养分与微生物功能多样性[J]. 林业科学, 2014, 50 (4): 22-31[Xiang ZY, Zhang L, Zhang QF, Liu W, Wang GX, Wang CT, Hu L. Soilnutrients and microbial functional diversity of different stand types inQinghai province [J]. Sci Silv Sin, 2014, 50 (4): 22-31]31 Hu CJ, Fu BJ, Liu GH, Jin TT, Liu Y. Soil microbial functional anddiversity under typical artificial woodlands in the hilly area of the LoessPlateau [J]. Acta Ecol Sin, 2009, 29 (2): 727-73332 Fang H, Yu YL, Chu XQ, Wang XG, Yang XE. Yu JQ. Degradationof chlorpyrifos in laboratory soil and its impact on soil microbialfunctional diversity [J]. J Environ Sci, 2009, 21: 380-38633 Yuan Y, Si G, Wang J, Luo T, Zhang G. Bacterial community in alpinegrasslands along an altitudinal gradient on the Tibetan Plateau [J].FEMS Microbiol Ecol, 2014, 87 (1): 121-13234 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2000: 25-82 [BaoSD. Analysis of Agricultural Chemistry [M]. Beijing: China AgriculturePress, 2000: 25-82]35 孔凡伟. 如何精测土壤容重[J]. 黑龙江农业科学, 2010 (10): 178-17836 刘祖祺. 植物抗逆生理学[M]. 北京: 中国农业出版社, 1994 [Liu ZQ.Plant Physiology [M]. Beijing: China Agriculture Press, 1994]37 王丽娟, 李刚, 赵建宁, 红雨, 修伟明, 王慧, 章秋艳, 杨殿林. 耐除草剂转基因大豆对根际土壤固氮微生物nifH基因多样性的影响[J]. 中国油料作物学报, 2013, 35 (6): 703-711 [Wang LJ, Li G, Zhao JN, HongY, Xiu WM, Wang H, Zhang QY, Yang DL. Effects of herbicide toleranttransgenic soybeans on the diversity of soil nitrogen-fixing bacterialnifH genes in rhizosphere [J]. Chin J Oil Crop Sci, 2013, 35 (6): 703-711]38 Konopka A, Oliver L, Turco R F. The use of carbon substrate utilizationpatterns in environmental and ecological microbiology [J]. Microb Ecol,1998, 35 (2): 103-11539 Deluca TH, Zouhar KL. Effects of selection harvest and prescribedfire on the soil nitrogen status of ponderosa pine forests [J]. For EcolManage, 2000, 138 (1): 263-27140 Guénon R, Vennetier M, Dupuy N, Roussos S, Pailler A, Gros R. Trendsin recovery of mediterranean soil chemical properties and microbialactivities after infrequent and frequent wildfires [J]. Land DegradationDev, 2013, 24 (2): 115-12841 杨桂生, 宋长春, 郭跃东, 路永正, 宋艳宇. 火烧对退耕还湿土壤微生物活性的影响[J]. 水土保持学报, 2010 (6): 218-221 [Yang GS,Song CC, Guo YD, Lu YZ, Song YY. Influence of fire on soil microbialactivity of restoration marsh [J]. J Soil Water Conservation, 2010 (6):218-221]42 Heisler JL, Seery A. Direct and indirect effects of fire on shrub densityand aboveground productivity in a Mesic Grassland [J]. Ecology, 2008,85 (8): 2245-225743 白爱芹, 傅伯杰, 曲来叶, 黄元元, 王丽华. 大兴安岭火烧迹地恢复初期土壤微生物群落特征[J]. 生态学报, 2012, 32 (15): 4762-4771[Bai AQ, Fu BJ, Qu LY, Huang YY, Wang LH. The characteristics ofsoil microbial communities at burned forest sites for the Great XinganMountains [J]. Acta Ecol Sin, 2012, 32 (15): 4762-4771]44 么中元, 张乃莉, 崔喜艳. 火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响[J]. 草地学报, 2014, 22 (4): 713-721 [YaoZY, Zhang NL, Cui XY. Impacts of fire and N addition on microbial Cutilization potential in a semi-arid grassland of Inner Mongolia [J]. ActaAgrestia Sin, 2014, 22 (4): 713-721]45 Klose S, Acosta-Martínez V, Ajwa HA. Microbial communitycomposition and enzyme activities in a sandy loam soil after fumigationwith methyl bromide or alternative biocides [J]. Soil Biol Biochem,2006, 38 (6): 1243-125446 Ladygina N, Hedlund K. Plant species influence microbial diversity andcarbon allocation in the rhizosphere [J]. Soil Biol Biochem, 2010, 42 (2):162-16847 孟庆杰, 许艳丽, 李春杰, 韩晓增, 裴希超. 不同施肥/土地利用方式对黑土细菌多样性的影响[J]. 大豆科学, 2008, 27 (3): 480-486 [MengQJ, Xu YL, Li CJ, Han XZ, Pei, XC. Effects of different fertilization andland use history on the bacterial diversity in black soils [J]. Soybean Sci,2008, 27 (3): 480-486]48 Docherty KM, Balser TC, Bohannan BJM, Gutknecht, JLM. Soilmicrobial responses to fire and interacting global change factors in aCalifornia annual grassland [J]. Biogeochem, 2012, 109 (1-3): 63-8349 Neary DG, Klopatek CC, Debano LF, Ffolliott PF. Fire effects onbelowground sustainability: a review and synthesis [J]. For EcolManage, 1999, 122: 51-7150 孙毓鑫, 吴建平, 周丽霞, 林永标, 傅声雷. 广东鹤山火烧迹地植被恢复后土壤养分含量变化[J]. 应用生态学报, 2009, 20 (3):513-517 [Sun,SX, Wu JP, Zhou LX, Lin YB, Fu SL. Changes of soilnutrient contents after prescribed burning of forestland in Heshan City,Guangdong Province [J]. Chin J Appl Ecol, 2009, 20 (3): 513-517]51 商丽娜, 吴正方, 杨青, 姜明, 刘吉平. 火烧对三江平原湿地土壤养分状况的影响[J]. 湿地科学, 2004, 2 (1): 54-60 [Shang LN, Wu FZ, YangQ, Jiang M, Liu JP. The effects of fire on the nutrient status of wetlandsoil in Sanjiang Plain [J]. Wetland Sci, 2004, 2 (1): 54-60]52 Hart SC, Deluca TH, Newman GS, Mackenzie MD, Boyle SI. Post-firevegetative dynamics as drivers of microbial community structure andfunction in forest soils [J]. For Ecol Manage, 2005, 220 (1-3): 166-184

相似文献/References:

[1]马驿,彭金菊,陈进军,等.诺氟沙星对土壤微生物碳代谢功能多样性的影响[J].应用与环境生物学报,2012,18(06):1033.[doi:10.3724/SP.J.1145.2012.01033]
　MA Yi,PENG Jinju,CHEN Jinjun,et al.Effect of Norfloxacin on Carbon-source Metabolic Functional Diversity of Soil Microbial Communities[J].Chinese Journal of Applied & Environmental Biology,2012,18(02):1033.[doi:10.3724/SP.J.1145.2012.01033]
[2]刘星,王娜,赵博,等.改变碳输入对太岳山油松林土壤酶活性的影响[J].应用与环境生物学报,2014,20(04):655.[doi:10.3724/SP.J.1145.2013.12021]
　LIU Xing,WANG Na,ZHAO Bo,et al.Effects of carbon input changes on soil enzyme activities in a Pinus tabulaeformis forest at the Taiyue Mountain[J].Chinese Journal of Applied & Environmental Biology,2014,20(02):655.[doi:10.3724/SP.J.1145.2013.12021]
[3]杨希智,王长庭,字洪标,等.三江源区不同建植年限人工草地土壤微生物群落结构特征[J].应用与环境生物学报,2015,21(02):341.[doi:10.3724/SP.J.1145.2014.10033]
　YANG Xizhi,WANG Changting,ZI Hongbiao,et al.Soil microbial community structure characteristics in artificial grassland with different cultivation years in the headwater region of Three Rivers, China[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):341.[doi:10.3724/SP.J.1145.2014.10033]
[4]魏晓骁 陈爱玲 王士亚 曹光球** 蔡培菁 黎 舒.杉木连栽林土壤微生物碳源利用比较?[J].应用与环境生物学报,2016,22(03):518.[doi:10.3724/SP.J.1145.2015.09010]
　WEI Xiaoxiao,CHEN Ailing,WANG Shiya,et al.A comparative study of soil microbial carbon source utilization in different successive rotation plantations of Chinese fir*[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):518.[doi:10.3724/SP.J.1145.2015.09010]
[5]崔羽,贺静雯,刘颖,等.红壤区灾害受损不同林型恢复过程中土壤微生物群落对碳源的利用[J].应用与环境生物学报,2019,25(04):831.[doi:10.19675/j.cnki.1006-687x.2018.12003]
　CUI Yu,HE Jingwen,et al.Utilization of carbon sources by soil microbial communities of different forest types damaged by flood-induced disasters in red soil regions[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):831.[doi:10.19675/j.cnki.1006-687x.2018.12003]
[6]余金阳,黄潇慧,帅正彬,等.四川彭州大蒜根腐病发病土壤细菌与真菌群落结构[J].应用与环境生物学报,2020,26(04):928.
　YU Jinyang,HUANG Xiaohui,SHUAI Zhengbin,et al.The community structure of bacteria and fungi in soils with root rot diseased garlic plants in Pengzhou, Sichuan Province[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):928.
[7]杨思,杨文平,景豆豆,等.麦后复种苜蓿压青还田改善土壤微生物群落结构[J].应用与环境生物学报,2021,27(04):978.[doi:10.19675/j.cnki.1006-687x.2020.11004]
　YANG Si,YANG Wenping,JING Doudou,et al.Improvement of soil microbial community structure by multiple cropping alfalfa after wheat[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):978.[doi:10.19675/j.cnki.1006-687x.2020.11004]
[8]张常洪 张英 朱晓敏** 尹华军.西南山地针叶林根际/菌丝际土壤氮动态对氮添加的响应差异[J].应用与环境生物学报,2022,28(06):1.[doi:10.19675/j.cnki.1006-687x.2021.11022]
　ZHANG Changhong,ZHANG Ying,et al.Differential responses of nitrogen dynamics between the r hizosphere and hyphosphere soils of mountain coniferous forests to N addition in southwest China[J].Chinese Journal of Applied & Environmental Biology,2022,28(02):1.[doi:10.19675/j.cnki.1006-687x.2021.11022]

更新日期/Last Update: 2016-04-25