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[1]苏军,李刚,吴明基,等.转Osepsps抗草甘膦水稻不同生境适合度及对稻田杂草的影响[J].应用与环境生物学报,2015,21(05):897-903.[doi:10.3724/SP.J.1145.2015.02027]
　SU Jun,LI Gang,WU Mingji,et al.Fitness of transgenic glyphosate-tolerant rice in different habitats and its influence on paddy field weeds[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):897-903.[doi:10.3724/SP.J.1145.2015.02027]
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转Osepsps抗草甘膦水稻不同生境适合度及对稻田杂草的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年05期
页码:
897-903
栏目:
研究论文
出版日期:
2015-10-25

文章信息/Info

Title:
Fitness of transgenic glyphosate-tolerant rice in different habitats and its influence on paddy field weeds
作者:
苏军;  李刚;  吴明基;  陈子强;  宋亚娜;  林智敏
福建省农业科学院生物技术研究所，福建省农业遗传工程重点实验室 福州 350003
Author(s):
SU Jun;  LI Gang;  WU Mingji;  CHEN Ziqiang;  SONG Yana;  LIN Zhimin
Biotechnology Institute of Fujian Academy of Agricultural Sciences, Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fuzhou 350003, China
关键词:
转基因水稻; 抗草甘膦; 生态适合度; 稻田杂草
Keywords:
transgenic rice;  glyphosate resistance;  fitness;  weeds
分类号:
S511.03 : Q788
DOI:
10.3724/SP.J.1145.2015.02027
文献标志码:
A
摘要:
携带适合度优势基因的转基因作物如果长期存活于栽培系统之外，抗性基因可能转移到野生近缘种并带来生态问题. 以转Osepsps基因水稻EP为材料，以非转基因受体“明恢86”为对照，通过田间定位试验，研究半野生生境下转Osepsps基因水稻的营养生长、繁殖能力以及稻田杂草的种类、频度和密度等指标变化，分析转Osepsps基因在半野生生境中的生态适合度以及对稻田杂草群落的影响. 连续2年试验的结果显示：在常规栽培和半野生生境下，转基因稻与非转基因稻的株高、分蘖数、单株穗数、单株总粒数、单株实粒重和千粒重等指标没有显著差异；但结实率显著受环境影响，半野生生境下转基因水稻2年结实率为50.33%和17.72%，相比对照的68.82%和30.27%均显著下降（P < 0.05）；2012年转基因稻单株实粒数为335粒，显著少于非转基因对照的548粒（P < 0.05）. 转基因稻与非转基因稻稻田的杂草种类均为9科9属9种；栽培稻田杂草密度和频度显著低于半野生稻田；相同生境下转基因稻与非转基因稻稻田杂草频度、密度总体上没有差异. 本研究表明，与非转基因对照相比，转epsps基因稻生态适合度没有显著提高，在半野生生境下有所下降，其生态风险不高于栽培稻.
Abstract:
When in a host outside cultivation for a long time, the fitness-conferring transgenes may be spread from an agricultural host to a weedy relative. This research aimed to assess the fitness of glyphosate-tolerant rice in habitats outside of cultivation. We planted transgenic and non-transgenic rice in semi wild habitat and cultivated plots, recorded the plant height, number of tillering as well as weeds species and numbers during the growing course, and investigated the reproduction index after harvesting. The results showed no significant differences between transgenic and non-transgenic lines under the same growing condition in plant height or number of tillers in vegetative period, or the reproduction indexes in maturity period including number of spikes per plant, total grain numbers per plant, grain weight per plant, and the weight of 1000 grains. However, the seed set ratio of transgenic rice was 50.33% in 2013 and 17.72% in 2012, obviously lower than that of non-transgenic rice (68.82% and 30.27% respectively) in semi wild habitat. In 2012 the filling grain numbers per plant of transgenic rice was 335 grains, significantly lower than the 548 grains of non-transgenic rice. For both transgenic and non-transgenic rice paddies, 9 species of weeds were recorded in semi wild habitat and cultivated plots, with no difference between. Field weed frequency and density were obviously lower in cultivated plots than in semi wild habitat, but with no difference between transgenic and non-transgenic fields. The results suggested that compared to non-transgenic rice, the fitness of transgenic rice was not improved but rather declined in semi wild habitat. Therefore transgenic rice brings with it no more ecological risk than non-transgenic ones when outside of cultivation.

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备注/Memo

备注/Memo:
转基因生物新品种培育科技重大专项（2014ZX08001-003）资助 Supported by the Key Technology Project of Transgenic New Species Cultivation (2014ZX08001-003)
更新日期/Last Update: 2015-10-29