|本期目录/Table of Contents|

[1]杨娟,谢翼飞,李旭东,等.一株耐盐硫氧化细菌的分离鉴定及脱硫机理[J].应用与环境生物学报,2015,21(05):819-823.[doi:10.3724/SP.J.1145.2015.02006]
 YANG Juan,XIE Yifei,LI Xudong,et al.Isolation, identification and desulfurization mechanism of a sulfur-oxidizing bacterium with salt tolerance[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):819-823.[doi:10.3724/SP.J.1145.2015.02006]
点击复制

一株耐盐硫氧化细菌的分离鉴定及脱硫机理()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年05期
页码:
819-823
栏目:
论文
出版日期:
2015-10-25

文章信息/Info

Title:
Isolation, identification and desulfurization mechanism of a sulfur-oxidizing bacterium with salt tolerance
作者:
杨娟 谢翼飞 李旭东 陈瑜江 赵玉萍
1中国科学院成都生物研究所,中国科学院环境与应用微生物重点实验室 成都 610041 2中国科学院大学 北京 100049
Author(s):
YANG Juan XIE Yifei LI Xudong CHEN Yujiang ZHAO Yuping
1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University?of?Chinese?Academy?of?Sciences,?Beijing?100049,?China
关键词:
硫氧化细菌(SOB)16S rDNA硫代硫酸盐生物脱硫氧化机理Thioalkalivibrio
Keywords:
sulfur-oxidizing bacterium 16S rDNA thiosulfate bio-desulfurization oxidation mechanism Thioalkalivibrio
分类号:
X172
DOI:
10.3724/SP.J.1145.2015.02006
文献标志码:
A
摘要:
筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义. 从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B. 根据16S rDNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定. 进一步研究DS-B的硫代硫酸盐耐受性,并通过测定培养体系中各价态含硫物质的含量,探讨该菌的硫氧化性能. 结果显示,菌株DS-B与Thioalkalivibrio thiocyanoxidans ARh2的序列相似性最高,初步鉴定其属于Thioalkalivibrio. DS-B的最佳生长和降解温度为35 ℃,35 ℃下培养7 d,硫代硫酸盐(S2O32-)的去除率能达到98.7%. 该菌对S2O32-有较强的耐受性,且最适S2O32-浓度为2 × 104 mg/L. 此外,对各价态硫氧化物的分析表明该菌氧化S2O32-的途径为:S2O32-→SO42- / S2O32-→S→SO42-. 上述表明,菌株DS-B具有较强的耐盐能力,对硫代硫酸盐具有较高的去除能力,是一株具有应用价值的硫氧化细菌.
Abstract:
Micro-organism with efficient desulfurization performance is a key factor in the biological desulfurization technology. This study aimed to seek such a sulfur-oxidizing strain and understand its desulfurization mechanism. Wastewater in a sewage station of natural gas purification plant was used to screen the sulfide-oxidizing strain, and to identify it based on sequence similarity analysis of 16S rDNA and the morphological characteristics. Thiosulfate was used as substrate for investigating the sulfur oxidation performance and salinity tolerance; the OD600, content change of thiosulfate, sulfate, sulfur, pH and total alkalinity in the cultural system were also investigated. The strain DS-B was found to share the highest sequence similarity with Thioalkalivibrio thiocyanoxidans ARh2, therefore determined as Thioalkalivibrio. At the optimum temperature of 35 oC for growth and degradation, the removal efficiency of thiosulfate could reach 98.7% after 7 days. Strain DS-B had strong resistance to thiosulfate, and the optimal concentration of S2O32- was 2 × 104 mg/L. The analysis for sulfur oxides showed that it could oxidize thiosulfate by the pathway of S2O32-→SO42- / S2O32- → S → SO42-. Therefore the strain DS-B is a sulfur-oxidizing bacterium with great application prospect for its strong salt tolerance and conspicuous removal capability for thiosulfate.

参考文献/References:

1 王钢, 王欣, 高德玉, 刘伟, 赫大新, 陈薇. 沼气生物脱硫技术研究[J]. 应用能源技术, 2005, 33 (5): 33-35 [Wang G, Wang X, Gao DY, Liu W, He DX, Chen W. Study on biological desulfurization technology for biogas [J]. Appl Energy Technol, 2005, 33 (5): 33-35]
2 李国欣, 杨俊仕, 李旭东. 天然气净化厂的废水处理设计与运行[J]. 中国给水排水, 2009, 25 (22): 49-55 [Li GX, Yang JS, Li XD. Design and operation of wastewater treatment process in natural gas purification plant [J]. China Water Wastewater, 2009, 25 (22): 49-55]
3 蒲万芬, 胡佩. 酸性气体中硫化氢的微生物脱除方法[J]. 天然气工业, 2005, 25 (3): 166-171 [Pu WF, Hu P. Method to remove hydrogen sulfide from sour gas by microorganisms [J]. Nat Gas Ind, 2005, 25 (3): 166-171]
4 汪家铭. Shell - Paques生物脱硫技术及其应用[J]. 化肥设计,2010, 48 (2): 39-42 [Wang JM. Shell - Paques biodesulfur technology and its application [J]. Chem Fertil Des, 2010, 48 (2): 39-42]
5 李红菊, 唐晓东, 赵红义, 郭巧霞. 天然气脱硫技术[J]. 石油化工腐蚀与防护, 2009, 26 (5): 4-6 [Li HJ, Tang XD, Zhao HY, Guo QX. Natural gas desulfurization technology [J]. Corr Prot Petrochem Ind, 2009, 26 (5): 4-6]
6 Buisman CJN, Wit B, Lettinga G. Biotechnological sulfide removal in three polyurethane carrier reactors: strirred reactor, bio-rotor reactor and up- flow reactor [J]. Water Res, 1990, 24 (2): 245- 251
7 李军幸, 张克强, 季民, 袁志华. 分光光度法测定微生物处理含硫化物废水中的单质硫含量[J]. 农业环境科学学报, 2006, 25 (1): 261-265 [Li JX, Zhang KQ, Ji M, Yuan ZH. Spectrophotometric quantification of sulfur in sulfide-containing wastewater treated with microbial [J]. J Agro Environ Sci, 2006, 25 (1): 261-265]
8 王蕙祥, 姜理英, 吴晓薇, 陈建孟. 硫氧化细菌的分离鉴定及降解特性[J]. 应用与环境工程学报, 2011, 17 (5): 706-710 [Wang HX, Jiang LY, Wu XW, Chen JM. Isolation, identification and degradation characteristics of a sulfide-oxidizing bacterium [J]. Chin J Appl Environ Biol, 2011, 17 (5): 706-710]
9 邓良伟, 唐一, 吴彦. 生物脱硫机理及其研究进展[J]. 上海环境科学, 1998, 17 (5): 35-39 [Deng LW, Tang Y, Wu Y. Mechanism of biological desulfurization and its progress [J]. Shanghai Environ Sci, 1998, 17 (5): 35-39]
10 顾运, 杨宏, 吴宣, 尚海源. 盐分对一株硫氧化细菌氧化能力的影响[J]. 环境工程学报, 2015, 9 (1): 491-494 [Gu Y, Yang H, Wu X, Shang HY. Effect of salinity on a sulfur-oxidizing bacteria oxidation ability [J]. Chin J Environ Eng, 2015, 9 (1): 491-494]
11 蒋永荣, 胡明成. 硫酸盐废水生物脱硫研究进展[J]. 环境科学与技术, 2008, 31 (9): 67-71 [Jiang YR, Hu MC. Biological desulfurization of sulfate containing wastewater [J]. Environ Sci Technol, 31 (9): 67-71]
12 王爱杰, 杜大仲, 任南琪, 赵秋实, 王文静. 脱氮硫杆菌在废水脱硫、脱氮处理工艺中的应用[J]. 哈尔滨工业大学学报, 2004, 36 (4): 423-425, 429 [Wang AJ, Du DZ, Ren NQ, Zhao QS, Wang WJ. Application of Thiobacillus denitrificans in wastewater denitrification and de-sulfide treatment processes [J]. J Harbin Inst Technol, 2004, 36 (4): 423-425, 429]
13 Pethkar AV, Paknikar KM. Thiosulfate biodegradation/silver biosorption process for the treatment of photofilm processing wastewater. Process Biochem, 2003, 38 (6): 855-860
14 Lin BL, Hosomi M, Murakami A. Effects of high salinity and constitute organic compounds on treatment of photo-processing waste by a sulphur-oxidizing bacteria/granular activated carbon sludge system [J]. Water Res, 2002, 36 (4): 1076-1083
15 林栋青, 张彦科, 顾向阳. 硫代硫酸盐氧化菌TX的分离、鉴定及其生物学特性[J]. 微生物学通报, 2009, 36 (11): 1638-1644 [Lin DQ, Zhang YK, Gu XY. Isolation, identification and characterization of thiosulfate-oxidizing bacterium TX [J]. Microbiology, 2009, 36 (11): 1638-1644
16 Baalsrud K, Kjellrun S Baalsrud. Studies on Thiobacillus denitrificans [J]. Archiv Microbiol, 1954, 20 (1): 34-62
17 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 353-356 [Dong XZ, Cai MY. System Identification Manual of Common Bacteria [M]. Beijing: Science Press, 2001: 353-356]
18 Mahmood Q, Hu B, Cai J,Zheng P, Azim MR, Jilani G, Islam E. Isolation of Ochrobacte-rium sp. QZ2 from sulfide and nitrite treatment system [J]. J Hazard Mater, 2009, 165 (1-3): 558-565
19 国家环保局. 水和废水监测分析方法[M]. 第4版. 北京: 中国环境科学出版社, 2002 [Ministry of Environmental Protection of China. Water and Wastewater Monitoring Analysis Method - IV [M]. Beijing: Chinese Environmental Science Press, 2002]
20 李军幸, 张克强, 季民, 袁志华. 分光光度法测定微生物处理含硫化物废水中的单质硫含量[J]. 农业环境科学学报, 2006, 25 (1): 261- 265 [Li JX, Zhang KQ, Ji M, Yuan ZH. Spectrophotometric quantification of sulfur in sulfide-containing wastewater treated with microbial [J]. J Agro Environ Sci, 2006, 25 (1): 261- 265]
21 沈镭, 张再丽, 贾晓珊. 氧化亚铁硫杆菌和氧化硫硫杆菌对硫代硫酸钠的代谢机理研究[J]. 环境科学学报, 2006, 26 (12): 2000-2007 [Shen L, Zhang ZL, Jia XS. Study on metabolic mechanisms of sodium thiosulfate by Thiobacillus Ferrooxidans and Thiobacillus Thiooxidans [J]. J Environ Sci, 2006, 26 (12): 2000-2007]
22 Solicio C, Lodi A, Veglio F. Bioleaching of zinc and aluminium from industrial waste sludge by means of Thiobacillus ferrooxidans [J]. Waste Mana, 2002, 22: 667- 675

相似文献/References:

[1]苏莹 陈莉 汪辉 刘兆普.海洋石油降解菌的筛选与降解特性[J].应用与环境生物学报,2008,14(04):518.
[2]洪璇,张晓君,孟智奇,等.废水反硝化生物反应器中喹啉降解细菌的分离与特性[J].应用与环境生物学报,2008,14(06):803.[doi:10.3724/SP.J.1145.2008.00803]
 HONG Xuan,ZHANG Xiaojun**,MENG Zhiqi,et al.Isolation and Characteristics of Quinoline Degrading Bacteria from Wastewater Denitrifying Bioreactor[J].Chinese Journal of Applied & Environmental Biology,2008,14(05):803.[doi:10.3724/SP.J.1145.2008.00803]
[3]付博,王卫卫,郝莹,等.紫花苜蓿根际氢氧化细菌的分离与鉴定[J].应用与环境生物学报,2009,15(05):650.[doi:10.3724/SP.J.1145.2009.00650]
 FU Bo,WANG Weiwei,HAO Ying,et al.Isolation and Identification of Hydrogen-oxidizing Bacteria in Medicago sativa Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):650.[doi:10.3724/SP.J.1145.2009.00650]
[4]张晓霞,马晓彤,曹卫东,等.紫云英根瘤菌的系统发育多样性[J].应用与环境生物学报,2010,16(03):380.[doi:10.3724/SP.J.1145.2010.00380]
 ZHANG Xiaoxia,MA Xiaotong,CAO Weidong,et al.Phylogenetic Diversity of Rhizobial Bacteria Isolated from Astragalus sinicus[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):380.[doi:10.3724/SP.J.1145.2010.00380]
[5]袁敏,张辉,胡国全.一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析[J].应用与环境生物学报,2010,16(05):705.[doi:10.3724/SP.J.1145.2010.00705]
 YUAN Min,ZHANG Hui,HU Guoquan.Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):705.[doi:10.3724/SP.J.1145.2010.00705]
[6]陈法霖,张凯,郑华,等.PCR-DGGE技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响[J].应用与环境生物学报,2011,17(02):145.[doi:10.3724/SP.J.1145.2011.00145]
 CHEN Falin,ZHANG Kai,ZHENG Hua,et al.Analyzing the Effect of Mixed Decomposition of Conifer and Broadleaf Litters on Soil Microbial Communities by Using PCR-DGGE[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):145.[doi:10.3724/SP.J.1145.2011.00145]
[7]吕早生,关海燕,李凌凌,等.一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定[J].应用与环境生物学报,2011,17(03):326.[doi:10.3724/SP.J.1145.2011.00326]
 LÜ,Zaosheng,GUAN Haiyan,et al.Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):326.[doi:10.3724/SP.J.1145.2011.00326]
[8]郭娜,李志敏,叶勤.烷烃降解菌的筛选、鉴定及优势菌株的降解特性[J].应用与环境生物学报,2011,17(04):572.[doi:10.3724/SP.J.1145.2011.00572]
 GUO Na,LI Zhimin,YE Qin.Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):572.[doi:10.3724/SP.J.1145.2011.00572]
[9]刘力嘉,马玉龙,谢丽,等.泰乐菌素降解菌的分离鉴定及其系统发育分析[J].应用与环境生物学报,2012,18(06):1038.[doi:10.3724/SP.J.1145.2012.01038]
 LIU Lijia,MA Yulong,XIE Li,et al.Isolation, Identification and Phylogenetic Analysis of a Tylosin-degrading Strain[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):1038.[doi:10.3724/SP.J.1145.2012.01038]
[10]陈丹丹,顾胜华,张金娜,等.肠道菌群对免疫系统的影响及其群落分析方法[J].应用与环境生物学报,2013,19(03):542.[doi:10.3724/SP.J.1145.2013.00542]
 CHEN Dandan,GU Shenghua,ZHANG Jinna,et al.Effect of Intestinal Microbes on the Immune System and the Latest Research Methods[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):542.[doi:10.3724/SP.J.1145.2013.00542]

备注/Memo

备注/Memo:
国家自然科学基金项目(51378013)和中国科学院STS计划项目资助 Supported by the National Natural Science Foundation of China (51378013) and the STS Program of Chinese Academy of Sciences
更新日期/Last Update: 2015-10-26