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[1]李强,李树红,李小林,等.野生翘鳞肉齿菌子实体内生菌多样性[J].应用与环境生物学报,2015,21(04):629-634.[doi:10.3724/SP.J.1145.2015.02004]
 LI Qiang,LI Shuhong,LI Xiaolin,et al.Diversity of endophytic microorganisms in fresh fruiting bodies of Sarcodon imbricatus[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):629-634.[doi:10.3724/SP.J.1145.2015.02004]
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野生翘鳞肉齿菌子实体内生菌多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年04期
页码:
629-634
栏目:
研究论文
出版日期:
2015-08-25

文章信息/Info

Title:
Diversity of endophytic microorganisms in fresh fruiting bodies of Sarcodon imbricatus
作者:
李强 李树红 李小林 陈诚 黄文丽 熊川 杨志荣 郑林用
1四川省农业科学院土壤肥料研究所 成都 610066 2四川大学生命科学学院 成都 610065 3云南省农业科学院生物技术与种质资源研究所 昆明 650223 4四川省农业科学院生物技术核技术研究所 成都 610066
Author(s):
LI Qiang LI Shuhong LI Xiaolin CHEN Cheng HUANG Wenli XIONG Chuan YANG Zhirong  ZHENG Linyong
1Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 2School of Life Sciences, Sichuan University, Chengdu 610065, China 3Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China 4Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
关键词:
翘鳞肉齿菌虎掌菌子实体内生菌多样性外生菌根
Keywords:
Sarcodon imbricatus fruiting bodies endophytes diversity ectotrophic mycorrhiza
分类号:
Q938.15
DOI:
10.3724/SP.J.1145.2015.02004
文献标志码:
A
摘要:
为了解翘鳞肉齿菌[Sarcodon imbricatus (L. ex Fr.) Karst.]子实体内生菌的多样性,以西藏、吉林的新鲜野生翘鳞肉齿菌子实体为材料,采用可培养方法分离并鉴定其中的内生细菌与真菌. 共从中分离出52株细菌、26株真菌. 鉴定结果显示,所有内生细菌分别隶属于变形菌门(Proteobacteria)及厚壁菌门(Firmicutes),变形菌门占96.2%,处绝对优势. 其中伯克氏菌属(Burkholderia)最多,占40.4%,假单胞菌属(Pseudomonas)次之,占23.1%. 26株真菌共分为14个属,Phaeomoniella 最多,占26.9%,隐球菌属(Cryptococcus)其次,占11.5%. 从菌柄及菌盖中分离的细菌、真菌种类及数量均有差异. 本研究分离的这些菌株具有很大的潜在利用价值,可为筛选具有特殊功效的菌株提供新的材料来源,同时也可为探索外生菌根菌与共生植物之间的物质交换与菌根合成打下基础.
Abstract:
This study aimed to understand the diversity of endophytic microorganisms in fresh bodies of Sarcodon imbricatus (L. ex Fr.) Karst. All together 10 wild fresh S. imbricatus samples were collected from the Tibetan Autonomous Region and Jilin Province. The diversity of endophytic microorganisms was investigated by culture-dependent method. A total of 52 strains of endophytic bacteria and 26 strains of endophytic fungi were isolated and identified from the friuiting bodies of S. imbricatus. All bacteria could be classified as Proteobacteria and Firmicutes, with Proteobacteria (96.2%) being the dominant phylum in the fruiting bodies of S. imbricatus, while Burkholderia (40.4%) the most in the level of genus, followed with Pseudomonas (23.1%). The 26 strains of fungi could be classified into 14 genera, with Phaeomoniella (26.9%) being the dominant genus, followed by Cryptococcus (11.5%). The species and quantities of bacteria and fungi isolated from the stipe and pileus varied significantly. The results suggested that these strains have great potential value. The study provides a new source to screen strains with special effect, and lays a foundation for exploring material exchange between ectotrophic mycorrhiza fungi and symbiotic plants.

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备注/Memo

备注/Memo:
四川省科技支撑计划项目(2013NZ0029,2014FZ0004)资助 Supported by the Science & Technology Pillar Project of Sichuan Province (2013NZ0029 & 2014FZ0004)
更新日期/Last Update: 2015-08-24