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[1]黄廷林,何秀秀,张海涵,等.水库贫营养异养硝化-好氧反硝化菌Sxf14的脱氮特性[J].应用与环境生物学报,2015,21(02):201-207.[doi:10.3724/SP.J.1145.2014.10002]
 HUANG Tinglin,HE Xiuxiu,ZHANG Haihan,et al.Nitrogen removal characteristics of the heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter sp. Sxf14[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):201-207.[doi:10.3724/SP.J.1145.2014.10002]
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水库贫营养异养硝化-好氧反硝化菌Sxf14的脱氮特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年02期
页码:
201-207
栏目:
研究论文
出版日期:
2015-04-25

文章信息/Info

Title:
Nitrogen removal characteristics of the heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter sp. Sxf14
作者:
黄廷林 何秀秀 张海涵 周石磊 白士远
西安建筑科技大学环境与市政工程学院 西安 710055
Author(s):
HUANG Tinglin HE Xiuxiu ZHANG Haihan ZHOU Shilei BAI Shiyuan
School of Environmental and Municipal Engineering, Xi’an University of Architecture & Technology, Xi’an 710055, China
关键词:
贫营养异养硝化好氧反硝化16S rRNA水库
Keywords:
oligotrophic heterotrophic nitrification aerobic dentification 16S rRNA reservoir
分类号:
X172
DOI:
10.3724/SP.J.1145.2014.10002
文献标志码:
A
摘要:
为利用生物强化法降低微污染源水中的氮素,从水库沉积物中筛选到一株好氧反硝化细菌Sxf14. 通过扫描电镜和16S rRNA序列分析,鉴定其为不动杆菌属(Acinetobacter sp.),命名为Acinetobacter sp. Sxf14. 同时,对该菌株脱氮特性进行研究,并将其接种到C/N(总有机碳与总氮的比值)为1.2的微污染水库源水中,以探究其对实际源水总氮的去除效果. 结果显示:Sxf14能以硝酸盐和亚硝酸盐为唯一氮源进行好氧反硝化. 反应48 h后,NO3--N和NO2--N的去除率分别达74.84(±0.86)%和40.52(±1.49)%,TN去除率最高达到65.07(±1.56)%和41.33(±0.98)%;在以NH4Cl为氮源的异养硝化系统内,该菌在48 h内使NH4+-N浓度由3.73(±0.08) mg/L降到1.28(±0.20) mg/L,氨氮去除率达到65.63(±1.39)%. 72 h内,微污染水库源水的TN浓度由2.46(±0.02) mg/L降到1.68(±0.01) mg/L,去除率达到31.7(±0.14)%. 因此,菌株Acinetobacter sp. Sxf14具有反硝化能力,能承受较低的碳氮比,降低微污染源水中的氮素,本研究可为微污染水体的菌剂修复技术提供科学依据.
Abstract:
In the present study, a heterotrophic nitrification-aerobic denitrification bacteria strain was isolated from the sediment of a drinking water reservoir. Based on Scan Electronic Microscope (SEM) and 16S rRNA gene sequence analyses, the strain was identified as Acinetobacter sp. and named Acinetobacter sp. Sxf14. The strain was inoculated into the micro-polluted source water (C/N as 1.2), and the total nitrogen (TN) removal rate was examined. The results showed that the strain could perform nitrification and denitrification well in nitrate medium, nitrite medium, and the reservoir water environment. The removal rate of nitrate reached 74.84±0.86% and that of nitrite reached 40.52±1.49% in 48 hours; the total nitrogen removal rates was up to 65.07±1.56% and 41.33± 0.98%. The concentration of NH4+-N reduced from 3.73±0.08 mg/L to 1.28±0.20 mg/L, with the removal rate up to 65.63±1.39%. In 72 h, the concentration of TN of micro-polluted source water reduced from 2.46±0.02 mg/L to 1.68±0.01 mg/L, with the removal rate up to 31.7±0.14%. The results of this work demonstrated that strain Sxf14, with its aerobic denitrification ability and tolerance low carbon nitrogen ratio, can decrease the nitrogen concentration of micro-polluted source water, therefore, and may be a promising agent in bioremediation for micro-polluted source water.

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备注/Memo

备注/Memo:
国家科技支撑计划课题(2012BAC04B02)和国家自然科学基金项目(51208413)资助 Supported by the National Science and Technology Pillar Program (2012BAC04B02) and the National Natural Science Foundation of China (51208413)
更新日期/Last Update: 2015-04-27