|本期目录/Table of Contents|

[1]匡双便,徐祥增,孟珍贵,等.不同透光率对三七生长特征及根皂苷含量的影响[J].应用与环境生物学报,2015,21(02):279-286.[doi:10.3724/SP.J.1145.2014.08002]
 KUANG Shuangbian,XU Xiangzeng,MENG Zhengui,et al.Effects of light transmittance on plant growth and root ginsenoside content of Panax notoginseng[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):279-286.[doi:10.3724/SP.J.1145.2014.08002]
点击复制

不同透光率对三七生长特征及根皂苷含量的影响()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年02期
页码:
279-286
栏目:
研究论文
出版日期:
2015-04-25

文章信息/Info

Title:
Effects of light transmittance on plant growth and root ginsenoside content of Panax notoginseng
作者:
匡双便 徐祥增 孟珍贵 张广辉 杨生超 陈中坚 魏富刚 陈军文
1云南农业大学云南省优势中药材规范化种植工程研究中心 昆明 650201 2云南农业大学农学与生物技术学院 昆明 650201 3文山学院三七研究院 文山 663000 4云南文山苗乡三七实业有限公司 文山 663000
Author(s):
KUANG Shuangbian XU Xiangzeng MENG Zhengui ZHANG Guanghui YANG Shengchao CHEN Zhongjian WEI Fugang CHEN Junwen
1Yunnan Research Center of Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming 650201, China 2College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China 3Institute of Panax notoginseng, Wenshan University, Wenshan 663000, China 4Wenshan Miaoxiang Panax notoginseng Industral Co. Ltd., Wenshan 663000, China
关键词:
三七光照强度生物量根系皂苷
Keywords:
Panax notoginseng light intensity biomass root ginsenoside
分类号:
Q945.79 : Q946.83
DOI:
10.3724/SP.J.1145.2014.08002
文献标志码:
A
摘要:
摘 要 为明确光照强度对药用作物一年生和二年生三七[Panax notoginseng (Burk) F. H. Chen]形态特征、生物量积累和分配以及皂苷积累的影响,为三七优质、高效栽培提供理论依据,采用搭建不同透光率遮荫棚的方法对三七进行人工遮荫,荫棚透光率分别为22.3%、11.8%、8.4%、2.9%和1.1%,在三七收获期分别测定植株的形态指标、生物量以及皂苷含量. 结果显示:当透光率在22.3%-2.9%之间时,透光率降低对一年生三七各器官生物量无显著性影响,但当透光率降低到1.1%时,各器官生物量显著降低;在透光率为1.1%的条件下,一年生三七根冠比和根质比显著减小,茎质比和叶质比显著增大. 二年生三七根生物量在透光率为22.3%、11.8%、8.4%和2.9%的条件下无显著差异,在透光率为1.1%的条件下显著降低. 透光率为11.8%的条件最有利于一年生和二年生三七根系的健康生长;但一年生三七根总皂苷含量在透光率为22.3%的条件下最高(质量分数w = 0.862%),二年生三七总皂苷含量在透光率为8.4%的条件下最高(质量分数w = 5.642%). 总的来看,三七是一种典型的喜阴根茎类药用植物,适当遮荫有利于其生长及皂苷的积累;10%左右的透光率应是三七生产中首选的生长环境光照强度.
Abstract:
In order to provide a scientific basis for efficient and high-quality cultivation of medicinal crop Panax notoginseng, we examined the morphology, biomass accumulation and allocation, as well as root ginsenoside accumulation in P. notoginseng grown along artificial light gradients. Seeds and one-year-old rootstocks were firstly sowed in nursery bed. After the seedlings came out evenly, healthy seedlings were transplanted into five screen houses where light intensity was 22.3%, 11.8 %, 8.4%, 2.9% and 1.1% of full sunlight, respectively. The morphology, biomass accumulation and allocation, and ginsenoside content of P. notoginseng were analyzed during the harvest period. The results showed no significant differences of root, stem, and leaf biomass of P. notoginseng among 22.3% to 2.9% of full sunlight, but significant decrease under 1.1% of full sunlight. Under 1.1% of full sunlight, the root/shoot ratio and root/biomass ratio significantly decreased, but the stem/biomass ratio and leaf/biomass ratio increased. The biomass of two-year-old P. notoginseng showed no significant differences among 22.3%, 11.8%, 8.4% and 2.9% of full sunlight, but significant decrease under 1.1% of full sunlight. Root of one- and two-year-old P. notoginseng grew vigorously under 11.8% of full sunlight. Ginsenoside content in one-year-old P. notoginseng reached a maximum value (w = 0.862%) under 22.3% of full sunlight, but the maximum value (w = 5.642%) of that in two-year-old P. notoginseng was under 8.4% of full sunlight. In conclusion, P. notoginseng is a typical shade-tolerant medicinal crop species; appropriate shading will facilitate its growth and ginsenoside accumulation; light intensity of about 10% of full sunlight is preferred for the production of P. notoginseng.

参考文献/References:

1 刁俊明, 曾宪录. 光强对桐花树根系生长和根系活力的影响[J]. 嘉应学院学报 (自然科学), 2013, 31 (8): 68-75 [Diao JM, Zeng XL. The effect of light intensity on root growth and root activity of Aegiceras corniculatum [J]. J Jiaying Univ (Nat Sci), 2013, 31 (8): 68-75]
2 Simone SD, Oka Y, Inoue Y. Effects of light on root hair formation in Arabidopsis thaliana phytochrome-deficient mutants [J]. J Plant Res, 2000, 113: 63-69
3 Wahl S, Rysey P, Edwards PJ. Phenotypic plasticity of grass root anatomy in response to light intensity and nutrient supply [J]. Ann Bot, 2001, 88: 1071-1078
4 Nagel KA, Schurr U, Water A. Dynamics of root growth stimulation in Nicotiana tabacum in increasing light intensity [J]. Plant Cell Environ, 2006, 29: 1936-1945
5 徐飞, 郭卫华, 徐伟红, 王仁卿. 不同光环境对麻栎和刺槐幼苗生长和光合特征的影响[J]. 生态学报, 2010, 30 (12): 3098-3107 [ Xu F, Guo WH, Xu WH, Wang RQ. Effects of light intensity on growth and photosynthesis of seedlings of Quercus acutissima and Robinia pseudoacacia [J]. Acta Ecol Sin, 2010, 30 (12): 3098-3107]
6 周卫霞, 董朋飞, 王秀萍, 李潮海. 弱光胁迫对不同基因型玉米籽粒发育和碳氮代谢的影响[J]. 作物学报, 2013, 39 (10): 1826-1834 [Zhou WX, Dong PF, Wang XP, Li CH. Effects of low-light stress on kernel setting and metabolism of carbon and nitrogen in different maize (Zea mays L.) genotypes. Acta Agron Sin, 2013, 39 (10): 1826-1834]
7 张吉旺, 董树亭, 王空军, 胡昌浩, 刘鹏. 大田遮阴对夏玉米淀粉合成关键酶活性的影响[J]. 作物学报, 2008, 34 (8): 1470-1474 [Zhang JW, Dong ST, Wang KJ, Hu CH, Liu P. Effects of shading in field on key enzymes involved in starch synthesis of summer maize [J]. Acta Agron Sin, 2008, 34 (8): 1470-1474]
8 崔海岩, 靳立斌, 李波, 赵斌, 董树亭, 刘鹏, 张吉旺. 大田遮阴对夏玉米光合特性和叶黄素循环的影响[J]. 作物学报, 2013, 39 (3): 478-485 [Cui HY, Jin LB, Li B, Zhao B, Dong ST, Liu P, Zhang JW. Effects of shading on photosynthetic characteristics and xanthophyll cycle of summer maize in the field [J]. Acta Agron Sin, 2013, 39 (3): 478-485]
9 王一, 杨文钰, 张霞, 雍太文, 刘卫国, 苏本营. 不同生育时期遮阴对大豆形态性状和产量的影响[J]. 作物学报, 2013, 39 (10): 1871-1879 [Wang Y, Yang WY, Zhang X, Yong TW, Liu WG, Su BY. Effects of shading at different growth stages on different traits and yield of soybean [J]. Acta Agron Sin, 2013, 39 (10): 1871-1879]
10 王培武, 李治远, 曦田昭弘, 魏国治, 涂华玉. 新疆大豆生产及生态的研究[J]. 作物学报, 1995, 21 (4): 396-403 [Wang PW, Li ZY, Akihiro I, Wei GZ, Xu HY. The study of soybean ecotypes and its production in Xinjiang [J]. Acta Agron Sin, 1995, 21 (4): 396-403]
11 赵桂茹, 王仕玉, 郭凤根, 李玉强, 张丽梅, 龙雯虹, 陈严平, 周平. 不同相对光照强度对岩白菜产量和品质的影响[J]. 西部林业科学, 2013, 42 (6): 104-108 [Zhao GR, Wang SY, Guo FG, Li YQ, Zhang LM, Long WM, Chen YP, Zhou P. Effects of different relative light intensity on yield and quality of Bergenia purpurascens [J]. J West China For Sci, 2013, 42 (6): 104-108]
12 吴春梅, 黎云祥, 权秋梅, 曾冉, 郭强. 淫羊藿果实成熟与生境的关系[J]. 应用与环境生物学报, 2008, 14 (2): 187-191 [Wu CM, Li YX, Quan QM, Zeng R, Guo Q. Relationship between fruit maturity and habitats of Epimedium bervicornum [J]. Chin J Appl Envion Biol, 2008, 14 (2): 187-191]
13 蒋妮, 覃柳燕, 李力. 环境胁迫对药用植物次生代谢产物的影响[J]. 湖北农业科学, 2012, 51 (8): 1528-1532 [ Jiang N, Tang LY, Li L. Influence of environmental stress on medicinal plant secondary metabolites [J]. Hubei Agric Sci, 2012, 51 (8): 1528-1532]
14 黄璐琦, 郭兰萍. 环境胁迫下次生代谢产物的积累及道地药材的形成[J]. 中国中药杂志, 2007, 32 (4): 277-280 [Huang LQ, Guo LP. Secondary metabolites accumulating and geoherbs formation under environmental stress [J]. Chin J Chin Mat Med, 2007, 32 (4): 277-280]
15 王丹, 王振月, 王宗权, 陈金铭. 药用植物次生代谢产物生产途径的研究概述[J]. 中医药信息, 2008, 25 (1): 29-32 [Wang D, Wang ZY, Wang ZQ, Chen JM. Brief on the progress of productive ways of the secondary metabolites in medicinal plants [J]. Trad Chin Med, 2008, 25 (1): 29-32]
16 康延国. 中药鉴定学[M]. 第2版. 北京: 中国中医药出版社, 2007
17 国家药典委员会编. 中华人民共和国药典(一部) [S]. 北京: 中国医药科技出版社, 2010: 11
18 陈晓亚, 汤章城主编. 植物生理与分子生物学[M]. 3版. 北京: 高等教育出版社, 2007: 329-356
19 黄璐琦, 陈美兰, 肖培根. 中药材道地性研究的现代生物学基础及模式假说[J]. 中国中药杂志, 2004, 29 (6):494-496 [Huang LQ, Chen ML, Xiao PG. The study of Chinese traditional medicine authentic modern biological basis and model hypothesis [J]. Chin J Chin Mat Med, 2004, 29 (6): 494-496]
20 Szakiel A, P?czkowski C, Henry M. Influence of environmental abiotic factors on the content of saponins in plants. Phytochemistry, 2011, 10: 471-491
21 陈中坚. 以“三七”名称命名的药用植物概述[J]. 特产研究, 2009 (2): 23-25 [Chen ZJ. Summarize of medicinal plants named “Panax notoginseng” [J]. Spec Wild Econ Anim Plant Res, 2009 (2): 23-25]
22 陈中坚, 崔秀明, 王朝梁, 王勇. 文山优质三七基地生态区划和布局研究[J]. 人参研究, 1999, 11 (3): 29-31
23 华劲松, 戴红燕, 夏明忠. 不同光照强度对芸豆光合特性及产量性状的影响[J]. 西北农业学报, 2009, 18 (2): 136-140 [Hua JS, Dai HY, Xia MZ. Effects of different light intensity on photosynthetic characteristic and yield characters of kidney bean [J]. Acta Agric Bor-occid Sin, 2009, 18 (2): 136-140]
24 李有志, 张灿明, 谢永宏, 黄继山, 杨刚. 三江平原小叶章和芦苇幼苗生长对低光胁迫的响应[J]. 应用与环境生物学报, 2009, 15 (1): 53-58 [Growth responses of Deyeuxia angustifolia and Phragmites communis seedlings to low-light stress in the Sanjiang plain [J]. Chin J Appl Envion Biol, 2009, 15 (1): 53-58]
25 孟金柳, 曾波, 叶小齐, 付天飞, 何兴兵. 不同光照水平下叶损失对樟生物量分配的影响[J]. 西南师范大学学报(自然科学版), 2004, 29 (3): 439-444 [ Meng JL, Zeng B, Ye XQ, Fu TF, He XB. The effect of defoliation on the biomass partitioning of Cinnamomum camphora under different light levels [J]. J Southwest China Normal Univ (Nat Sci Edn), 2004, 29 (3): 439-444]
26 王艺, 韦小丽. 不同光照对植物生长、生理生化和形态结构影响的研究进展[J]. 山地农业生物学报, 2010, 29 (4): 353-359 [Wang Y, Wei XL. Advance on the effects of different light environments on growth, physiological biochemistry and morphostructure of plant [J]. J Mount Agric Biol, 2010, 29 (4): 353-359]
27 张永刚, 韩梅, 韩忠明, 刘翠晶, 杨利民. 不同生境朝鲜淫羊藿生长与光合特征[J]. 生态学报, 2012, 32 (5): 1442-1449 [Zhang YG, Han M, Han ZH, Liu CJ, Yang LM. Growth and photosynthetic characteristics of Epimedium koreanum Nakai in different habitats [J]. Acta Ecol Sin, 2012, 32 (5): 1442-1449]
28 黎开强, 吴卫, 郑有良, 代勇, 向丽, 廖凯. 不同光强对川贝母生长发育和总生物碱的影响[J]. 中草药, 2009, 40 (9): 1475-1478 [ Li KQ, Wu W, Zheng YL, Dai Y, Xiang L, Liao K. Effects of light intensity on growth and total alkaloids of Fritillari acirrhosa [J]. Chin Tradit Herb Drugs, 2009, 40 (9): 1475-1478]
29 何维明, 钟章成. 攀援植物绞股蓝幼苗对光照强度的形态和生长反应[J]. 植物生态学报, 2000, 24 (3): 375-378 [He WM, Zhong ZC. Morphological and growth responses of the climbing plant, Gynostemma petaphyllum seedlings to varying light intensity [J]. Acta Phytoecol Sin, 2000, 24 (3): 375-378]
30 蔡昆争, 骆世明, 董桃杏. 水稻根系生理生态研究进展[C]//杨文钰. 作物栽培生理研究文集. 北京: 中国农业出版社, 2008: 27-38
31 李明财, 朱教君, 孙荣. 东北次生林主要树种比叶面积对光照强度的响应[J]. 生态学杂志, 2009, 28 (8): 1437-1442 [ Li MC, Zhu JJ, Sun R. Responses of specific leaf area of dominant tree species in northeast china secondary forests to light intensity [J]. Chin J Ecol, 2009, 28 (8): 1437-1442]
32 蔡昆争, 郎有忠, 葛少彬, 林威鹏, 王维, 蔡一霞, 文晓慧, 王冰清, 陈怡. 作物根系生理生态学[M]. 北京: 化学工业出版社, 2010: 163-166
33 李润, 刘绍文, 潘俊锋, 郭蓓蓓, 曾莉, 王燕, 肖勇, 杨丽. 光照强度对马铃薯脱毒试管苗的影响[J]. 农业科技通讯, 2013, 10: 83-85
34 Mawaki M, Morito S, Suga T. Effect of shading on root system morphology and grain yield of rice plant: I. An analysis on root length density [J]. Jpn J Crop Sci, 1990a, 59 (1): 89-94
35 Mawaki M, Harada J, Iwata T. Effect of shading on root system morphology and grain yield of rice plant: II. An analysis on primary roots [J]. Jpn J Crop Sci, 1990b, 59 (1): 95-99
36 Morita S, Yamazaki K. Effects of light condition on growth angle of rice roots grown with leaf-cutting method [J]. J Engl Acad Purp, 1992, 61 (1): 689-690
37 陈晓亚. 植物次生代谢研究[J]. 世界科技研究与发展, 2006, 28 (5): 1-4
38 郭继明, 淮虎银. 药用植物与环境[M]. 北京中国医药科技出版社, 1997: 56
39 于国华, 筒辉民, 罗文熹. 不同光照强度对西洋参光合特性营养成分和产量的影响[J]. 应用生态学报, 1994, 5 (1): 57 [Yu GH, Tong HM, Luo WX. Effects of light intensity on photosynthetic characteristic nutrients and yield of Panax quinquefolium [J]. Chin J Appl Ecol, 1994, 5 (1): 57]
40 文涛, 梁莉, 曾杨, 喻晓. 不同光照强度对虎杖愈伤组织的影响[J]. 中国中药杂志, 2007, 32 (13): 1277-1280 [Weng T, Liang L, Zeng Y, Yu X. Effect of different light intensity on Polygonum cuspidatumcallus [J]. China J Chin Mat Med, 2007, 32 (13): 1277-1280]
41 王志清, 郑培和, 逄世峰, 关一鸣, 郭靖, 王英平. 光照强度对北细辛生长发育及质量的影响[J]. 中国中药杂志, 2011, 36 (12): 1558-1567 [Wang ZQ, Zheng PH, Feng SF, Guang YM, Guo J, Wang YP. Effects of light intensity on growth and quality of Asarum heterotropoides var. mandshuricum [J]. China J Chin Mata Med, 2011, 36 (12): 1558-1567]
42 王万贤, 杨毅, 邓铭, 柯文山, 定明芹, 戴为苹. 生态因子对绞股蓝总皂苷含量影响的研究[J]. 中草药, 1996, 27 (9): 559-561
43 焦晓林, 高微微. 环境因子对药用植物三萜皂苷合成影响的研究进展[J]. 中草药, 2011, 42 (2): 398-402 [Jiao XL, Gao WW. Advances in studies on influence of environmental factors on triterpenoid saponin synthesis in medicina1 Plants [J]. Chin Trad Herb Drugs, 2011, 42 (2): 398-402]
44 Park H. Physiological response of Panax ginseng to light [C]. Proceeding of the 3rd international ginseng symposium. Seoul: Korean Ginseng Research Institute, 1980: 151-170
45 张治安, 徐克章, 任跃英, 陈星. 光照条件对参株碳水化合物和人参皂甙含量的影响[J]. 吉林农业大学学报, 1994, 16 (3): 15-17 [Zhang ZA, Xu KZ, Ren YY, Chen X. Effect of light intensity on content of soluble sugar, starch and ginseng saponin in ginseng plant [J]. J Jilin Agric Univ, 1994, 16 (3): 15-17]

相似文献/References:

[1]李有志,张灿明,谢永宏,等.三江平原小叶章和芦苇幼苗生长对低光胁迫的响应[J].应用与环境生物学报,2009,15(01):53.[doi:10.3724/SP.J.1145.2009.00053]
 LI Youzhi,ZHANG Canming,XIE Yonghong,et al.Growth Responses of Deyeuxia angustifolia and Phragmites communis Seedlings to Low-light Stress in the Sanjiang Plain*[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):53.[doi:10.3724/SP.J.1145.2009.00053]
[2]柯德森,杨礼香,巫锦雄.光强对玉米幼苗光合特性及环境羟基自由基水平的影响[J].应用与环境生物学报,2013,19(03):404.[doi:10.3724/SP.J.1145.2013.00404]
 KE Desen,YANG Lixiang,WU Jinxiong.Effects of Light Intensity on the Photosynthetic Characteristics of Maize Seedlings and the Content of Atmosphere Hydroxyl Radical[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):404.[doi:10.3724/SP.J.1145.2013.00404]
[3]祖艳群,孙晶晶,闵强,等.二年生三七中黄酮含量对砷胁迫的响应及其酶学机理[J].应用与环境生物学报,2014,20(06):1005.[doi:10.3724/SP.J.1145.2014.04034]
 ZU Yanqun,SUN Jinjin,MIN Qiang,et al.Response of flavonoid content in Panax notoginseng to As and its enzymological mechanism[J].Chinese Journal of Applied & Environmental Biology,2014,20(02):1005.[doi:10.3724/SP.J.1145.2014.04034]
[4]孙莹,石锦安,邵小鹏,等.不同生境条件下光照强度对蓝花楹光合色素含量及开花的影响[J].应用与环境生物学报,2015,21(06):1150.[doi:10.3724/SP.J.1145.2015.07036]
 SUN Ying,SHI Jinan,SHAO Xiaopeng,et al.Effects of light intensity on photosynthetic pigment content and flowering of Jacaranda mimosifolia D. Don in different habitats[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):1150.[doi:10.3724/SP.J.1145.2015.07036]
[5]付 忠 谢世清 徐文果 岩 所 陈军文*.3种光强环境下白魔芋生长旺盛期的光合和叶绿素a荧光特征[J].应用与环境生物学报,2016,22(03):446.[doi:10.3724/SP.J.1145.2015.10013]
 FU Zhong,XIE Shiqing,XU Wenguo,et al.Characteristics of photosynthesis and chlorophyll a fluorescence in Amorphophallus albus during vigorous growth under different light intensity*[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):446.[doi:10.3724/SP.J.1145.2015.10013]
[6]葛娜,杨玲,陈军文.不同浓度赤霉素和脱落酸对顽拗性三七种子后熟种胚发育和内源激素的影响[J].应用与环境生物学报,2020,26(03):574.[doi:10.19675/j.cnki.1006-687x.2019.08033]
 GE Na,,et al.Effects of varying gibberellin and abscisic acid levels on embryo development and endogenous hormones in recalcitrant Panax notoginseng seeds during the after-ripening process[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):574.[doi:10.19675/j.cnki.1006-687x.2019.08033]

备注/Memo

备注/Memo:
国家自然科学基金项目(81360609)和云南省中青年学术技术带头人后备人才培养项目(2014HB011)资助 Supported by the National Natural Science Foundation of China (81360609 ) and the Project of Young and Middle-aged Talent of Yunnan Province (2014HB011)
更新日期/Last Update: 2015-04-28