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[1]陈颖,刘柿良,杨容孑,等.镉胁迫对龙葵生长、质膜ATP酶活性及氮磷钾吸收的影响[J].应用与环境生物学报,2015,21(01):121-128.[doi:10.3724/SP.J.1145.2014.07028]
 CHEN Ying,LIU Shiliang,YANG Rongjie,et al.Effects of cadmium on growth, plasma membrane ATPase activity, and absorption of N, P and K in Solanum nigrum L. seedlings[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):121-128.[doi:10.3724/SP.J.1145.2014.07028]
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镉胁迫对龙葵生长、质膜ATP酶活性及氮磷钾吸收的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年01期
页码:
121-128
栏目:
研究论文
出版日期:
2015-02-25

文章信息/Info

Title:
Effects of cadmium on growth, plasma membrane ATPase activity, and absorption of N, P and K in Solanum nigrum L. seedlings
作者:
陈颖 刘柿良 杨容孑 张思寻 罗承德
1四川农业大学林学院 雅安 625014 2四川农业大学风景园林学院 成都 6111302 3成都职业技术学院 成都 610041
Author(s):
CHEN Ying LIU Shiliang YANG Rongjie ZHANG Sixun LUO Chengde
1Forestry College, Sichuan Agricultural University, Ya’an 611130, China 2Landscape Architecture College, Sichuan Agricultural University, Chengdu 611130, China 3Chengdu Polytechnic, Chengdu 610041, China
关键词:
龙葵镉胁迫质膜过氧化养分吸收ATP 酶活性抗氧化酶
Keywords:
Solanum nigrum L. cadmium stress lipid peroxidation nutrients absorption ATPase activities antioxidative enzymes
分类号:
X173 : Q945.78
DOI:
10.3724/SP.J.1145.2014.07028
文献标志码:
A
摘要:
采用模拟镉(Cd)污染土壤培养法研究不同浓度Cd (0、10、20、40、80、160 mg kg–1)处理对Cd超积累植物龙葵(Solanum nigrum)幼苗营养元素氮(N)、磷(P)、钾(K)吸收及质膜ATP酶活性的影响. 结果表明,Cd处理浓度≤40 mg kg–1时显著促进龙葵幼苗的生长(叶性状、主根长、株高度和基径粗度)以及生物量的积累与分配;而当Cd处理浓度>40 mg kg–1时则出现明显的抑制作用. 而当Cd处理浓度为10 mg kg–1时,则提高显著幼苗叶片叶绿素(Chl. a、Chl. b、Chl. [a+b])含量,达到最高值;且叶绿素含量随胁迫程度的增强而先升后降. 随胁迫程度的增强,幼苗根、茎、叶和果实中的N、P和K含量先升后降(除茎P降低外);而植株组织中的Cd积累量逐渐增大且分布为叶>茎>根>果实. 同时,丙二醛(MDA)含量与过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性随Cd浓度增大而增大,但超氧化物歧化酶(POD)活性先升后降. 随胁迫程度的增强,幼苗地上(茎与叶)和地下(根)部H+-ATP以及地下部Ca2+-ATP酶活性不断降低,而地上部Ca2+-ATP酶活性先升高后降低. 因此,龙葵在高浓度Cd胁迫(≥40 mg kg–1)下,可能通过加快根对Cd离子的吸收和转运,提高抗氧化酶(CAT和SOD)活性,降低POD与质膜ATP酶活性,调节对N、P 和K的需求,从而起到对Cd胁迫的解毒作用.
Abstract:
This study aimed to investigate by a soil pot experiment the effects of cadmium (Cd) stress on the plasma membrane ATP activity and absorption of nutrients including nitrogen (N), phosphorus (P) and potassium (K) in the hyperaccumulator plant Solanum nigrum. The results showed that low-dose Cd treatment (≤ 40 mg kg–1) enhanced the accumulation and distribution of biomass, thus promoting overall plant growth. On the other hand, high-dose Cd (> 40 mg kg–1) significantly inhibited the growth of S. nigrum seedlings. Moreover, 10 mg kg–1 Cd treatment increased leaf chlorophyll a (Chl. a), chlorophyll b (Chl. b) and total chlorophyll (Chl. [a+b]) content. With the increase of Cd dose, these chlorophyll contents showed a trend of?increase?followed by?decrease, same with that of N, P and K content in seedling tissues (roots, stems, leaves and fruits) except for the stem P content). With the increase of Cd doses, the Cd accumulation of root, stem, leaf and fruit all gradually increased (leaf > stem > root > fruit). In addition, the content of malondialdehyde (MDA), and the activities of catalase (CAT) and superoxide dismutase (SOD) also increased with increasing Cd doses, while the activity of peroxidase (POD) showed a trend of?increase?followed by decrease. Simultaneous with increasing Cd doses, shoot (leaf plus stem) and root H+–ATPase activities and root Ca2+-ATPase activity decreased, but the activity of shoot Ca2+-ATPase first increased and then decreased. These results suggested that S. nigrum plants may alleviate Cd detoxification under higher dose of Cd treatment (≧40 mg kg–1) through accelerating the absorption and transportation of root Cd ions, improving antioxidant enzymes (CAT and SOD) activity, decreasing POD and plasma membrane ATP activity and regulation N, P, K demand.

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备注/Memo

备注/Memo:
国家“十一五”重大科技支撑计划项目(2006BACO1A11)和四川农业大学创新团队计划项目(00370501)资助 Supported by the Key Sci-tech Project of the Eleventh Five Year Plan of China (2006BACO1A11) and the Innovation Team Program of Sichuan Agricultural University (00370501)
更新日期/Last Update: 2015-02-15