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[1]李自龙,焦健,徐雪风,等.硝酸型和硫酸型酸胁迫下油橄榄离体叶片的抗性生理比较[J].应用与环境生物学报,2014,20(06):999-1004.[doi:10.3724/SP.J.1145.2014.04035]
 LI Zilong,JIAO Jian,XU Xuefeng,et al.A comparison of resistant physiology of olive excised leaves under stresses of nitric-rich acid and sulphur-rich acid[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):999-1004.[doi:10.3724/SP.J.1145.2014.04035]
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硝酸型和硫酸型酸胁迫下油橄榄离体叶片的抗性生理比较()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年06期
页码:
999-1004
栏目:
研究论文
出版日期:
2014-12-31

文章信息/Info

Title:
A comparison of resistant physiology of olive excised leaves under stresses of nitric-rich acid and sulphur-rich acid
作者:
李自龙 焦健 徐雪风 任丽娟 杨别亮 陈东 王开祥 李朝周
1甘肃省作物遗传改良和种质创新重点实验室 兰州 730070 2甘肃农业大学生命科学技术学院 兰州 730070 3甘肃农业大学林学院 兰州 730070
Author(s):
LI Zilong JIAO Jian XU Xuefeng REN Lijuan YANG Bieliang CHEN Dong WANG Kaixiang LI Chaozhou
1Gansu Key Laboratory of Crop Genetics & Germplasm Enhancement, Lanzhou 730070, China 2College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China 3Forestry College, Gansu Agricultural University, Lanzhou 730070, China
关键词:
油橄榄酸胁迫硝酸型硫酸型抗性生理
Keywords:
Olea europaea acid stress nitric-rich acid sulphur-rich acid resistant physiology
分类号:
Q945.78
DOI:
10.3724/SP.J.1145.2014.04035
文献标志码:
A
摘要:
基于我国酸雨类型正逐渐从硫酸型趋向硝酸型转化,分别以含有较多硫酸根的硫酸、硝酸混合液(Sulphur-rich acid,SRA)和较多硝酸根的硫酸、硝酸混合液(Nitric-rich acid,NRA)浸泡离体油橄榄叶片,模拟硫酸型、硝酸型两种类型的酸胁迫,探讨不同酸度不同胁迫时间油橄榄离体叶片抗性生理的差异及其变化机理. 结果表明,随着胁迫时间延长,叶片丙二醛(MDA)含量显著增加(P < 0.05),叶绿素和类胡萝卜素含量降低,可溶性糖含量先增后减,脯氨酸含量和细胞膜稳定指数(MSI)下降;对比这两种类型的酸胁迫,pH为3的酸胁迫48 h时NRA酸胁迫叶绿素含量较SRA酸胁迫低10.12%(以硝酸型相关数据为基数,下同),类胡萝卜素含量、MSI下降幅度及MDA含量升高幅度均大于SRA型酸胁迫,说明NRA型酸胁迫造成了较为严重的色素分解、脂质过氧化和细胞膜损伤. 酸胁迫还引起超氧阴离子产生速率和过氧化氢含量增加,且硝酸型酸胁迫活性氧水平升高较大;抗氧化酶活性先变大后变小,维生素C含量和还原型谷胱甘肽含量均减小,且NRA酸胁迫减小幅度大于SRA,说明NRA酸胁迫比SRA酸胁迫对油橄榄叶片具有较强的氧化性,对抗氧化酶、类胡萝卜素、还原型谷胱甘肽等构成的细胞膜保护系统损害更大,从而加重了细胞膜的损伤,如pH为3的酸胁迫48 h时,NRA酸胁迫MSI较SRA 酸胁迫低26.52%. 可见,在较强的酸溶液(pH 3.0)胁迫较长时间时,硝酸型酸胁迫相对于硫酸型酸胁迫对细胞内的抗氧化系统具有较强的破坏,并由此造成了较为严重的细胞膜损伤.
Abstract:
Acid rain is a worldwide pollution problem and one of the major environmental issues. This research aimed to elucidate the effects of acid rain on plant and its mechanism, by comparing the resistant physiology of olive excised leaves under the stresses of nitric-rich acid and sulphur-rich acid. Olive leaves were treated with sulphur-rich acid (SRA) and nitric-rich acid (NRA) solutions respectively to compare the damage mechanism and resistant physiology under both stresses. The results showed that under stress the content of MDA significantly increased (P < 0.05), the content of chlorophyll, carotenoid, proline and MSI decreased, the content of soluble sugar increased initially then declined. After 48 h of acid stress with pH of 3, the chlorophyll content was 10.12% lower in NRA acid stress than in SRA acid stress, the membrane stability index of NRA acid stress 26.52% lower than that of SRA acid stress. The carotenoid content and cell membrane stability index decreased more under the NRA stress than under the SRA stress. Acid stress also caused increase of the ultra oxygen anion producing rate and hydrogen peroxide content, with the level of superoxide anion increasing more under NRA stress than under SRA stress. The activity of antioxidant enzymes first increased and then decreased; the contents of Vc and reductive glutathione decreased more sharply under NRA stress than under SRA stress. These results were closely related to the fact that the oxidative ability of the dilute nitric acid is much higher than that of the dilute sulphur acid. The results suggested that the NRA acid stress has stronger damage on intracellular antioxidant system than the SRA acid stress, and results in more serious cell membrane damage.

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备注/Memo

备注/Memo:
国家科技支撑计划课题项目(2012BAD06B03)和甘肃省农牧厅项目(GNCX-2012-47)资助 Supported by the National Science and Technology Pillar Program Project of China (2012BAD06B03) and the Project of Agriculture and Animal Husbandry Department of Gansu Province (GNCX-2012-47)
更新日期/Last Update: 2015-01-05