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[1]李虎,廖丹,苏建强,等.外来种互花米草根内细菌多样性及功能[J].应用与环境生物学报,2014,20(05):856-862.[doi:10.3724/SP.J.1145.2014.03027]
　LI Hu,LIAO Dan,SU Jianqiang,et al. Diversity and function of endophytic bacteria in roots of exotic plant Spartina alterniflora[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):856-862.[doi:10.3724/SP.J.1145.2014.03027]
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外来种互花米草根内细菌多样性及功能()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
856-862
栏目:
研究论文
出版日期:
2014-10-25

文章信息/Info

Title:
 Diversity and function of endophytic bacteria in roots of exotic plant Spartina alterniflora
作者:
 李虎;  廖丹;  苏建强;  黄福义;  洪有为
1中国科学院城市环境研究所城市环境与健康重点实验室 厦门 361021
2厦门华厦职业学院 厦门 361024
3中国科学院大学 北京 100049
Author(s):
 LI Hu;  LIAO Dan;  SU Jianqiang;  HUANG Fuyi;  HONG Youwei
1Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
2Xiamen Huaxia Vocational College, Xiamen 361024, China
3University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
 ; 内生细菌; 多样性; 互花米草; 根
Keywords:
 ; endophytic bacteria;  diversity;  Spartina alterniflora;  root
分类号:
Q938.1 : S45
DOI:
10.3724/SP.J.1145.2014.03027
文献标志码:
A
摘要:
外来种互花米草（Spartina alterniflora）是滨海湿地生态系统的重要组成部分. 通过构建16S rRNA基因文库，研究福建省九龙江红树林自然保护区中互花米草根内细菌组成和多样性. 研究发现：互花米草根内细菌主要分为4个门，其中变形菌门（Proteobacteria）占57.69%，其次是拟杆菌门（Bacteroidetes）和梭菌（Clostridia）；可分为9个纲，其中γ变形菌纲（γ-Proteobacteria）类群在文库中所占比例最大，为25.96%；其次是δ变形菌纲（δ-Proteobacteria）、拟杆菌纲（Bacteroidetes）和梭菌纲（Clostridia）. 同时，克隆文库中包含与降解多环芳烃（PAHs）、脱硫、甲烷氧化、碳循环及固氮相关的菌属，且存在大量与数据库同源性比对低于95%的序列. 该结果可为互花米草入侵机制探讨和相关功能微生物的分离和研究提供参考依据.
Abstract:
Spartina alterniflora have substantial influence on the biogeochemical cycle of carbon, nitrogen, sulfur and organic pollutants in wetland ecosystems. The objective of this study was to explore the mechanism of S. alterniflora invasion by investigating the diversity and functions of endophytic bacteria in roots. The roots of S. alterniflora were collected from Jiulong River Estuary Provincial Mangrove Reserve. The total DNA of endophytic bacteria were extracted and 16S rRNA genes amplified followed by clone library analysis. The results showed a high bacterial diversity inside roots of S. alterniflora. The bacteria community consisted of four main phylums, with proteobacteria dominant with a proportion of 57.7%, followed by bacteroidete and Clostridia. The endophytic bacterial flora was composed of nine classes, in which γ-proteobacteria had the largest proportion of 25.96%, followed by δ-proteobacteria, bacteroidetes and clostridia. About 66% sequences in the clone library had similarity less than 95% to their closest match in GenBank. Some sequences in the clone library were closely related to the bacteria capable of polycyclic aromatic hydrocarbons degradation, desulfidation, methane-oxidizing, and nitrogen fixation. The endophytic bacterial community in roots of S. alterniflora is diverse and the high proportion of low similarity sequence indicated that the S. alterniflora endophyte is largely unexplored.

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备注/Memo

备注/Memo:
 国家自然科学基金项目（41006064）、福建省青年基金项目（2012J05081）、宁波科技计划项目（2014A610100）和国家留学基金项目（201304910080）资助 Supported by the National Natural Science Foundation of China (41006064), the Fujian Youth Science Foundation (2012J05081), the Natural Science Foundation of Ningbo (2014A610100) and the State Scholarships Foundation of China (201304910080)
更新日期/Last Update: 2014-10-30