|本期目录/Table of Contents|

[1]郭亚萍,张国庆,陈青君,等. 双孢蘑菇堆肥过程中细菌群落结构分析[J].应用与环境生物学报,2014,20(05):832-839.[doi:10.3724/SP.J.1145.2014.03020]
 GUO Yaping,ZHANG Guoqing,CHEN Qingjun,et al. Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):832-839.[doi:10.3724/SP.J.1145.2014.03020]
点击复制

 双孢蘑菇堆肥过程中细菌群落结构分析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
832-839
栏目:
研究论文
出版日期:
2014-10-25

文章信息/Info

Title:
 Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique
作者:
 郭亚萍张国庆陈青君杨凯
1北京农学院植物科学技术学院农业应用新技术北京市重点实验室 北京 102206
2北京农学院生物科学与工程学院农业部都市农业(北方)重点实验室 北京 102206
Author(s):
 GUO Yaping ZHANG Guoqing CHEN Qingjun YANG Kai
1College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China
2College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
关键词:
 双孢蘑菇堆肥细菌群落结构PCR-DGGE
Keywords:
 PCR-DGGE Agaricus bisporus compost bacterial community
分类号:
S141.4 : Q938
DOI:
10.3724/SP.J.1145.2014.03020
文献标志码:
A
摘要:
分别以玉米秸、牛粪和稻草、牛粪为主要原料,设计两种双孢蘑菇(Agaricus bisporus)堆肥配方并进行堆肥发酵,研究二者堆肥过程中细菌群落多样性. 在建堆、一次发酵结束和二次发酵结束3个时期分别采集堆肥样品,提取总DNA,以大肠杆菌16S rDNA序列设计通用引物,进行PCR-DGGE扩增和序列分析. 结果获得56条特异条带16S rDNA基因信息(GenBank登录号为KF630598-KF630653),分属于细菌的7个门、42个属. 两种配方堆肥过程中主要类群属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria),主要的优势种属包括Bacillus属、Paenibacillus属、Thermomonospora属、Thermasporomyces属、Pseudomonas属和Cellvibrio属. 多样性指数分析显示,在堆肥过程中,稻草配方微生物多样性变化呈连续上升趋势,而玉米秸配方堆肥微生物多样性呈先升高、再降低的趋势. 主成分分析(PCA)显示,玉米秸配方一次发酵结束的样品与二次发酵结束的样品聚为一类,说明玉米秸配方堆肥比预期提前腐熟. 本研究表明,稻草配方微生物多样性高,所需腐熟时间长;玉米秸配方微生物多样性略低,但所需腐熟时间大大缩短.
Abstract:
This study aimed to investigate the bacterial communities in mushroom compost using straw, corn stalks, and cow dung as major compost matrix. Bacterial communities of samples at the beginning of composting, at the end of fermentation phase I and II were collected and analyzed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA universal primers from Escherichia coli. A total of 56 different clone sequences were obtained (GenBank accession numbers of KF630598-KF630653). They were classified into 7 phyla and 43 genus. Dominant microflora during composting belonged to phyla Proteobacteria, Firmicutes, and Actinobacteria, with the dominant genus of Bacillus, Paenibacillus, Thermomonospora, Thermasporomyces, Pseudomonas, and Cellvibrio. Bacterial diversity (Shannon index) analysis showed that bacterial species in straw treatment continuously increased during composting, while those in corn stalks treatment firstly increased and then reduced. Principal component analysis showed that corn stalk compost at the end of fermentation phase I and phase II were clustered into one group, suggesting that corn stalk composts faster than anticipated. In general, straw compost has higher bacterial diversity but longer composting time, while corn stalk compost has lower bacterial diversity but shorter composting time.

参考文献/References:

 

1 王德芝, 刘瑞芳, 马兰, 林向群, 张淑霞. 现代食用菌生产技术[M]. 武汉: 华中科技大学出版社, 2012 [Wang DZ, Liu RF, Ma L, Lin XQ, Zhang SX. Modern Mushroom Production Technology [M]. Wuhan: Huazhong University of Science and Technology Press, 2012]<br/>

2 许修宏, 李洪涛, 张迪. 堆肥微生物学原理及双孢蘑菇栽培[M]. 北京: 科学出版社, 2009 [Xu XH, Li HT, Zhang D. Compost Microbiology Principles and Agaricus bisporus Cultivation [M]. Beijing: Science Press, 2009]<br/>

3 Sharma SS, Yons GL, Chambers J. Comparison of the changes in mushroom (Agaricus bisporus) compost during windrow and bunker stages of phase I and [J]. Assoc Appl Biol, 2000, 136: 59-68<br/>

4 Wagner M, Amann R, Lemmer H, Schleifer KH. Probing activated sludge with oligonucleotides specific for Proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure[J]. Appl Environ Microbiol, 1993, 59: 1520-1525<br/>

5 Gerard M, Kornelia S. Application of denaturing gradientgel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology [J]. Anton Leeuw, 1998, 73: 127-141<br/>

6 Novinscak A, Nadine JD, Celine S, Martin F. Characterization of bacterial and fungal communities in composted biosolids over a 2 year period using denaturing gradient gel electrophoresis [J]. Can J Microbiol, 2009, 55: 375-387<br/>

7 Wu L, Li F, Deng CY, Xu DQ, Jiang SW, Xiong YZ. Amethod for obtaining DNA from compost[J]. Appl Microbiol Biotechnol, 2009, 84: 389-395<br/>

8 Zeng GM, Yu Z, Chen YN, Zhang JC, Li H, Yu M , Zhao MJ. Response of compost maturity and microbial community composition to pentachlorophenol (PCP)-contaminated soil during composting [J]. Bioresource Technol, 2011, 102: 5905-5911 <br/>

9 王小芬, 王伟东, 高丽娟, 崔宗均. 变性梯度凝胶电泳在环境微生物研究中的应用详解[J]. 中国农业大学学报, 2006, 11 (5): 1-7 [Wang XF, Wang WD, Gao LJ, Cui ZJ. Protocols of applification of denaturing gradient gel electrophoresis (DGGE) in studies of environmental microorganism [J]. J China Agric Univ, 2006, 11 (5): 1-7]<br/>

10 Raquel B, Felícitas V, Antoni S. Dehydrogenase activity as a method for monitoring the composting process [J]. Bioresource Technol, 2008, 99: 905-908<br/>

11 何丽鸿, 陈明杰, 潘迎捷. 采用变性梯度凝胶电泳研究双孢蘑菇培养料后发酵过程中的细菌群落结构[J]. 微生物学报, 2009, 49 (2): 227-232 [He LH, Chen MJ, Pan YJ. Bacterial communities in the phase of Agaricus bisporus compost by denaturing gradient gel electrophoresis [J]. Acta Microbiol Sin, 2009, 49 (2): 227-232]<br/>

12 Székely AJ, Sipos R, Berta B, Vajna B, Hajdú C. DGGE and T-RFLP analysis of bacterial succession during mushroom compost production and sequence-aided T-RFLP profile of mature compost [J]. Microbial Ecol, 2009, 57: 522-533<br/>

13 贾洋洋. 利用宏基因组方法分析堆肥生境中微生物区系的变化[D]. 济南: 山东大学, 2012 [Jia YY. Microbial diversity analysis in composting environment by metagenomic method [D]. Jinan: Shandong University, 2012]<br/>

14 Xiao Y, Zeng GM, Yang ZH, Ma YH, Huang C, Shi WJ, Xu ZY, Huang J, Fan CZ. Effects of continuous thermophilic composting (CTC) on bacterial community in the active composting process [J]. Environ Microbiol, 2011, 62: 599-608<br/>

15 Agnolucci M, Cristani C, Battini F, Palla M. Microbially-enhanced composting of olive mill solid waste (wet husk): bacterial and fungal community dynamics at industrial pilot and farm level [J]. Bioresource Technol, 2013, 134: 10-16<br/>

16 席北斗, 刘鸿亮, 白庆中, 黄国, 曾光明, 李英军. 堆肥中纤维素和木质素的生物降解研究现状[J]. 环境污染治理技术与设备, 2002, 3 (3): 19-23 [Xi BD, Liu HL, Bai QZ, Huang GH, Zeng GM, Li YJ. Study on current status of lignin and cellulose biodegradation in composting process [J]. Techniq Equip Environ Pollut Control, 2002, 3 (3): 19-23]<br/>

17 Keiko W, Norio N, Tatsuki T, Norio K. The dominant bacteria shifted from the order ‘‘Lactobacillales’’ to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent [J]. World J Microbiol Biotechnol, 2009, 25: 803-811<br/>

18 王鸿磊, 王红艳, 宋俊芬, 丁强, 徐康铭, 邹积华. 双孢菇培养料工厂化发酵过程中微生物及物质变化研究[J]. 安徽农业科学, 2011, 39 (1): 94-96 [Wang HL, Wang HY, Song JF, Ding Q, Xu KM, Zhou JH. Variation of microorganisms and physicochemical properties of Agaricus bisporus compost [J]. J Anhui Agric, 2011, 39 (1): 94-96]<br/>

19 刘佳, 李婉, 许修宏, 李洪涛. 接种纤维素降解菌对牛粪堆肥微生物群落的影响[J]. 环境科学, 2011, 32 (10): 3073-3081 [Liu J, Li W, Xu XH, Li HT. Effect of cellulose-decomposing strain on microbial community of cow manure compost [J]. Environ Sci, 2011, 32 (10): 3073-3081]<br/>

20 姜成, 王泽生. 蘑菇培养料堆制过程中微生物的演替及作用[J]. 微生物学杂志, 2003, 23 (1): 56-58 [Jiang C, Wang ZS. The development and functions of microorganisms in the composting process for Agaricus bisporus cultivation [J]. J Microbiol, 2003, 23 (1): 56-58]<br/>

21 Gerben S, Tineke W, Olijnsm A, Gerrits PG. Inoculation of Scytalidium thermophilum in button mushroom compost and its effect on yield [J]. Appl Environ Microbial, 1994, 60 (9): 3049-3054<br/>

22 Wiegant WM. Growth characteristics of the thermophilic fungus Scytalidium thermophilum in relation to production of mushroom compost [J]. Appl Environ Microbial, 1992, 58: 1301-1307<br/>

23 Zarenejad F, Yakhchali B, Rasooli I. Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production [J]. World J Microbiol Biotechnol, 2012, 28 (1): 99-104<br/>

24 吴小建. 嗜热真菌的分离鉴定及其在双孢蘑菇栽培中的应用研究[D]. 南宁: 广西大学, 2012 [Wu XJ. Isolation and identification of thermophilic fungi and cultivation of Agaricus bisporus on substrates pre-colonized by Scytaidum thermophilum [D]. Nanning: Guangxi University, 2012]<br/>

25 席北斗, 党秋玲, 魏自民, 李鸣晓, 夏训峰, 杨天学, 陈凤先. 生活垃圾化堆肥对放线菌群落的影响[J]. 农业工程学报, 2011, 27: 228-23 [Xi BD, Dang QL, Wei ZM, Li MX, Xia XF, Yang TX, Chen FX. Effects of microbial inoculants on actinomycetes communities diversity during municipal solid waste composting [J]. Trans CSAE, 2011, 27: 228-223]<br/>

26 王晶, 许修宏. 利用PCR-DGGE方法研究添加复合菌剂对堆肥微生物群落的影响[J]. 农业环境科学学报, 2011, 30 (12): 2602-2607 [Wang J, Xu XH. Effects of adding to complex bacteria on microbial community of compost assessed by PCR-DGGE techniques [J]. J Agro-Environ Sci, 2011, 30 (12): 2602-2607]

相似文献/References:

[1]徐梦宇,刘艳杰,林晖,等.假单胞菌催化的碳碳双键不对称还原[J].应用与环境生物学报,2014,20(05):798.[doi:10.3724/SP.J.1145.2014.03034]
 XU Mengyu,LIU Yanjie,LIN Hui,et al. Asymmetric reduction of C=C bond catalyzed with Pseudomonas species[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):798.[doi:10.3724/SP.J.1145.2014.03034]
[2]吴金鑫,宗红,陆信曜,等.高效催化合成3-羟基丙酸的菌株特性[J].应用与环境生物学报,2014,20(05):804.[doi:10.3724/SP.J.1145.2014.03003]
 WU Jinxin,ZONG Hong,LU Xinyao,et al. Characterization of a strain catalyzing biosynthesis of 3-hydroxypropionic acid[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):804.[doi:10.3724/SP.J.1145.2014.03003]
[3]刘艳,徐岳松,吴茜,等. 雨生红球藻培养和产油脂工艺的优化[J].应用与环境生物学报,2014,20(05):809.[doi:10.3724/SP.J.1145.2014.01036]
 LIU Yan,XU Yuesong,WU Qian,et al. Optimization of Haematococcus pluvialis culture and lipid production process[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):809.[doi:10.3724/SP.J.1145.2014.01036]
[4]杨云喜,李佩,徐岳松,等. 产抗菌肽乳酸菌的分离、鉴定及培养条件优化[J].应用与环境生物学报,2014,20(05):817.[doi:10.3724/SP.J.1145.2013.12044]
 YANG Yunxi,LI Pei,XU Yuesong,et al. Isolation and identification of antimicrobial peptides-producing lactic acid bacteria and optimization of the culture conditions[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):817.[doi:10.3724/SP.J.1145.2013.12044]
[5]程才璎,刘晓风,袁月祥,等.酱香型白酒酒曲和连续七轮次堆积酒醅的细菌群落结构[J].应用与环境生物学报,2014,20(05):825.[doi:10.3724/SP.J.1145.2014.03035]
 CHENG Caiying,LIU Xiaofeng,YUAN Yuexiang,et al. Bacterial community structure in distiller’s yeast and accumulated fermented grains of Maotai-flavor liquor[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):825.[doi:10.3724/SP.J.1145.2014.03035]
[6]毕京芳,黄钧,关梦龙,等. 微生物菌剂发酵中草药渣生产有机肥[J].应用与环境生物学报,2014,20(05):840.[doi:10.3724/SP.J.1145.2014.03051]
 BI Jingfang,HUANG Jun,GUAN Menglong,et al. Composting Chinese herbal residues with inoculum of microbial agents to produce organic fertilizer[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):840.[doi:10.3724/SP.J.1145.2014.03051]
[7]贺文君,李甫,杨春艳,等. 川滇蔷薇果实中的三萜类化学成分[J].应用与环境生物学报,2014,20(05):846.[doi:10.3724/SP.J.1145.2014.02022]
 HE Wenjun,LI Fu,YANG Chunyan,et al. Studies on the triterpenoids from Rosa soulieana fruit[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):846.[doi:10.3724/SP.J.1145.2014.02022]
[8]李虎,廖丹,苏建强,等.外来种互花米草根内细菌多样性及功能[J].应用与环境生物学报,2014,20(05):856.[doi:10.3724/SP.J.1145.2014.03027]
 LI Hu,LIAO Dan,SU Jianqiang,et al. Diversity and function of endophytic bacteria in roots of exotic plant Spartina alterniflora[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):856.[doi:10.3724/SP.J.1145.2014.03027]
[9]徐佳奕,刘守海,徐兆礼,等. 三沙湾浮游动物群落对水团季节变化的响应[J].应用与环境生物学报,2014,20(05):869.[doi:10.3724/SP.J.1145.2014.01038]
 XU Jiayi,LIU Shouhai,XU Zhaoli,et al.Responses of zooplankton community to changes in water masses in the Sansha Bay[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):869.[doi:10.3724/SP.J.1145.2014.01038]
[10]原秀云,刘金龙,曾晓茂,等. 3种麻蜥MHC I类基因外显子3和外显子4序列特征[J].应用与环境生物学报,2014,20(05):877.[doi:10.3724/SP.J.1145.2014.02003]
 YUAN Xiuyun,LIU Jinlong,ZENG Xiaomao,et al. Characterization of major histocompatibility complex (MHC) class I loci exon 3 and exon 4 in three racerunner species (Squamata: Lacertidae: Eremias)[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):877.[doi:10.3724/SP.J.1145.2014.02003]

备注/Memo

备注/Memo:
 “十二五”国家科技支撑计划项目(2012BAD14B09)、现代农业产业技术体系北京市食用菌创新团队建设(PXM2013-014207-000096)和北京高等学校青年英才计划项目(YETP1714)资助 Supported by the Key Sci-tech Project of the 12th 5-year-plan of China (2012BAD14B09), the Beijing Innovative Grant of Modern Agricultural Technology System (PXM2013-014207-000096) and the Beijing Higher Education Young Elite Teacher Project (YETP1714)
更新日期/Last Update: 2014-10-30