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[1]刘艳,徐岳松,吴茜,等. 雨生红球藻培养和产油脂工艺的优化[J].应用与环境生物学报,2014,20(05):809-816.[doi:10.3724/SP.J.1145.2014.01036]
 LIU Yan,XU Yuesong,WU Qian,et al. Optimization of Haematococcus pluvialis culture and lipid production process[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):809-816.[doi:10.3724/SP.J.1145.2014.01036]
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 雨生红球藻培养和产油脂工艺的优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
809-816
栏目:
研究论文
出版日期:
2014-10-25

文章信息/Info

Title:
 Optimization of Haematococcus pluvialis culture and lipid production process
作者:
 刘艳 徐岳松 吴茜 宋廷富 张白梅 乔代蓉 曹毅 徐辉
 四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065
Author(s):
 LIU Yan XU Yuesong WU Qian SONG Tingfu ZHANG Baimei QIAO Dairong CAO Yi XU Hui
 Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China
关键词:
 雨生红球藻培养条件产油脂工艺油脂含量生物柴油
Keywords:
 Haematococcus pluvialis culture condition oil production process oil content biodiesel
分类号:
TK63 : Q949.206
DOI:
10.3724/SP.J.1145.2014.01036
文献标志码:
A
摘要:
为解决生物柴油生产中油脂短缺的问题,利用实验室筛选到的高产油脂藻株——雨生红球藻SCTCC-600012,进行培养条件优化和产油脂工艺研究,分别比较不同浓度的NaNO3、K2HPO4、α-萘乙酸(NAA)、3-吲哚丁酸(3-IBA)、Mg2+、葡萄糖对雨生红球藻SCTCC-600012生长及产油的影响. 结果显示,其最适生长条件分别为NaNO3 1.0 g/L,K2HPO4 1.0 g/L,NAA 7 mg/L,3-IBA 16 mg/L,Mg2+ 2.5 mg/L. 最适产油条件分别为NaNO3 0.125 g/L,K2HPO4 0.1 g/L,NAA 7 mg/L,3-IBA 1.0 mg/L,Mg2+ 2.5 mg/L,葡萄糖为1.0 g/L. 优化后的油脂含量为32.8%,比基础发酵培养基中的油脂含量(19.59%)提高了1倍左右. 利用GC-MS对油脂进行脂肪酸成分分析,发现主要成分为软脂酸和油酸,与植物油相似,适合用于生产生物柴油. 本研究通过优化培养条件和产油工艺,提高了雨生红球藻的生长速率和油脂含量,可为选择生物柴油原料提供参考.
Abstract:
This study aimed to solve the problem of oil shortage during the production of biodiesel, and to explore its culture technology. A microalgae strain named Haematococcus pluvialis SCTCC-600012 was screened from our laboratory due to its high oil production rate. We investigated the effects of different concentration of NaN03, K2HPO4, NAA, 3-IBA, Mg2+ and glucose on the biomass and oil content of Haematococcus pluvialis. The optimum condition for growth was NaNO3 1.0 g/L, K2HPO4 1 g/L, NAA 7 mg/L, 3-IBA 16 mg/L, Mg2+ 2.5 mg/L, and glucose 1.0 g/L, while the optimum condition for oil production was NaNO3 0.125 g/L, K2HPO4 0.1 g/L, NAA 7 mg/L, 3-IBA 1 mg/L, Mg2+ 2.5 mg/L, and glucose 1.0 g/L. The oil content after optimization was 32.8%, nearly double that in the normal fermentation medium. In addition, through the fatty acid composition analysis by GC-MS, we found that the fatty acid composition of the lipid was palmitic acid and oleic acid, similar to that of plant oil, suitable for biodiesel production. This experiment improved the growth rate and oil content of Haematococcus pluvialis. To some extent, it can ease the problem of oil shortage and provide reference for new material selection for biodiesel production.

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备注/Memo

备注/Memo:
 “十二五”国家支撑计划项目(2011BAD14B05,2013BAD10B01,2014BAD02B02)、国家科技基础条件平台项目(NIMR-2014-8)和四川省科技厅项目(2014GZX0005,2013HH0007,2013GZX0161-3,2013GZ0058,2013JCPT003)资助 Supported by the Sci-tech Pillar Project of the Twelfth Five-year Plan of China (2011BAD14B05, 2013BAD10B01, 2014BAD02B02), the National Sci-tech Infrastructure and Facility Development Program of China (NIMR-2014-8), and the Science and Technology Department of Sichuan Province (2014GZX0005, 2013HH0007, 2013GZX0161-3, 2013GZ0058, 2013JCPT003)
更新日期/Last Update: 2014-10-30