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[1]黄廷林,邸诗雨,张海涵,等.西安市典型景观水体水质与微生物种群结构多样性[J].应用与环境生物学报,2014,20(04):697-703.[doi:10.3724/SP.J.1145.2013.12003]
 HUANG Tinglin,DI Shiyu,ZHANG Haihan,et al.Water quality and diversity of microbial community structure of Xi’an typical scenic water bodies[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):697-703.[doi:10.3724/SP.J.1145.2013.12003]
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西安市典型景观水体水质与微生物种群结构多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年04期
页码:
697-703
栏目:
研究论文
出版日期:
2014-08-25

文章信息/Info

Title:
Water quality and diversity of microbial community structure of Xi’an typical scenic water bodies
作者:
黄廷林 邸诗雨 张海涵 周子振 周娜
西安建筑科技大学环境与市政工程学院 西安 710055
Author(s):
HUANG Tinglin DI Shiyu ZHANG Haihan ZHOU Zizhen ZHOU Na
School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China
关键词:
景观水体水体水质微生物群落巢式PCR-DGGE克隆测序
Keywords:
scenic water water quality microbial community nested PCR-DGGE clone sequence
分类号:
X832 + X172
DOI:
10.3724/SP.J.1145.2013.12003
文献标志码:
A
摘要:
选取西安市区9个景观水体,在分析水体水质指标基础上,运用巢式PCR-DGGE和克隆测序技术研究景观水体细菌和真菌的群落结构多样性. 结果表明,9个景观水体均为劣Ⅴ类水. 水质指标中总氮(TN)含量最高为丰庆公园,为11.286 mg/L,是曲江池的5倍;硝氮(NO3-N)含量最高为革命公园,为5.614 mg/L,是曲江池的5.6倍;氨氮(NH4-N)含量最高为丰庆公园,为3.442 mg/L,是大明宫太液池的12倍;高锰酸盐指数(CODMn)最高为丰庆公园,为38.886 mg/L,是曲江池的3.2倍. 巢式PCR-DGGE图谱主成分分析(PCA)结果显示,莲湖公园、永阳公园和大明宫太液池水体细菌种群相似度高,丰庆公园、新纪元公园、曲江池和革命公园水体细菌种群多样性相似,而兴庆湖水体细菌群落结构与其他水体差异显著(P < 0.05). 莲湖公园、永阳公园和大明宫太液池水体细菌种群结构主要受水体NO3-N、TN和DO含量的调控,其中NO3-N含量影响显著;NH4-N含量对革命公园、新纪元公园和曲江池水体细菌种群影响显著,其中NO3-N和CODMn呈现负相关关系. CODMn含量对兴庆湖水体细菌种群多样性影响显著. 大明宫太液池、莲湖公园、丰庆公园、新纪元公园和兴庆湖水体真菌种类高度相似,革命公园水体真菌群落结构与其他水体存在差异,种群受到TN、NO3-N和NH4-N的综合调控. NO2-N含量对永阳公园水体真菌种群影响最大,同时与酸碱度(pH)和溶解氧(DO)含量呈现负相关. 茎点霉属(Phoma sp.)为革命公园水体中优势真菌. 可见,西安市典型景观水体悬浮细菌和真菌群落结构特征受到不同水质指标的综合调控. 表4 图2 参27
Abstract:
Nine landscape water bodies were selected to explore the water quality and determine the water bacterial and fungal communities with nested PCR-DGGE and clone sequence techniques. The results showed that the nine landscape water bodies were of inferior Ⅴ water quality. The highest total nitrogen (TN) was 11.286 mg/L in Fengqing Park (FQ), five times higher than that of Qujiang Pool (QJ). Meanwhile, the highest nitrate (NO3-N) was 5.614 mg/L in Geming Park (GM) water body, which was 3.2 times higher than that of QJ. The highest ammonia nitrogen (NH4-N) was 3.442 mg/L found in FQ water body, which was 12 times higher than that of Daming Palace (DMG). The chemical oxygen demand (CODMn) of FQ was 38.886 mg/L, which was 3.2 times higher than that of QJ. The results of nested PCR-DGGE and PCA analyses suggested that Lianhu Park (LH), Yongyang Park (YY) and DMG were of similar water communities, FQ, Xinjiyuan Park (XJY), QJ and GM of similar diversity of water bacterial community. However, water bacterial community in Xingqing Lake (XQ) was different from other water bodies (P < 0.05). The water bacterial communities in Lianhu (LH), YY and DMG were regulated by NO3-N, TN and dissolved oxygen (DO) concentrations. The water bacterial community in GM, XJY and QJ was significantly influenced by NH4-N concentration, with a negative correlation between NO3-N and CODMn. The CODMn concentration had significant influence on XQ water bacterial community diversity. DMG, LH, FQ, XJY and XQ had similar water fungal community structure, however, GM had a significant different fungal community from other water bodies, which was regulated by TN, NO3-N and NH4-N. YY water fungal community was influenced by NO2-N concentration. Phoma sp. was the dominated water fungal species. The water bacterial and fungal community structures in typical scenic water in Xi’an City were regulated by different water quality indicators. The results of the present work will provide a basis for researching water microbial community structure in urban landscape water body in China.

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备注/Memo

备注/Memo:
国家科技支撑计划课题项目(2012BAC04B02)资助 Supported by the National Science and Technology Pillar Program of China (2012BAC04B02)
更新日期/Last Update: 2014-08-26