|本期目录/Table of Contents|

[1]巴顿,张琦,季秀玲,等.两株嗜热异养氨氧化细菌的鉴定及其氨氧化特性[J].应用与环境生物学报,2014,20(01):157-161.[doi:10.3724/SP.J.1145.2014.00157]
 BA Dun,ZHANG Qi,JI Xiuling,et al.Identification and characterization of two thermophilic heterotrophic ammonia-oxidizing bacterial strains[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):157-161.[doi:10.3724/SP.J.1145.2014.00157]
点击复制

两株嗜热异养氨氧化细菌的鉴定及其氨氧化特性()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年01期
页码:
157-161
栏目:
研究简报
出版日期:
2014-02-25

文章信息/Info

Title:
Identification and characterization of two thermophilic heterotrophic ammonia-oxidizing bacterial strains
作者:
巴顿张琦季秀玲魏云林林连兵
昆明理工大学生命科学与技术学院 昆明 650500
Author(s):
BA Dun ZHANG Qi JI Xiuling WEI Yunlin LIN Lianbing
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500,, China
关键词:
异养氨氧化细菌嗜热菌菌株分离鉴定
Keywords:
heterotrophic ammonia-oxidizing bacteria thermophilic bacteria isolation and identification
分类号:
Q939.9 : Q935
DOI:
10.3724/SP.J.1145.2014.00157
文献标志码:
A
摘要:
为寻找适用于堆肥的嗜热异养氨氧化细菌,采用稀释涂布平板法和格里斯试剂显色法筛选嗜热异养氨氧化细菌,利用含NH4+培养基研究其最佳生长温度、pH条件以及其氨氧化特点和NO2-、NO3-的生成速率,并通过16S rDNA测序及Blast比对确定其种属. 结果显示,从大理洱源热泉中分离了两株嗜热异养氨氧化细菌菌株NC8和NJ26;这两株菌的最适生长温度为55 ℃,最适生长pH为8.0;在最适生长条件下,NC8和NJ26菌株对NH4+的最大单位体积减少率分别为1 033.3 mg/L和1 183.67 mg/L;对NO2-和NO3-最大单位体积产生率分别为23.79 mg/L、272.30 mg/L和33.81 mg/L、366.70 mg/L;种属鉴定表明,NC8和NJ26分别为Bacillus属的不同种. 本研究表明,NC8和NJ26为异养型氨氧化细菌,能将NH4+氧化成NO2-和NO3-,以NO3-为主要积累产物,因此有望应用于堆肥过程中以减少氨气排放,同时提高堆肥中氮素的含量.
Abstract:
This research aimed to screen the thermophilic heterotrophic ammonia-oxidizing bacteria which will be used in compost to identify their ammonia-oxidizing characteristics. Dilute spread plate method and Griess reagent coloration method were used to screen thermophilic heterotrophic ammonia-oxidizing bacteria, and medium with NH4+ used to identify the optimum growth temperature and pH value, rates of ammonia-oxidizing, nitrite- and nitrate-producing of the ammonia-oxidizing bacteria. 16S rDNA sequencing, blast-comparison and phylogenetic analysis were used to compare the ammonia-oxidizing bacteria. Two heterotrophic thermophilic ammonia-oxidizing bacterial strains, NC8 and NJ26, were isolated from hot spring zone in Eryuan, Yunnan. Their 16S rDNA sequence analysis showed that they belonged to different species of genus Bacillus. For both strains, the optimum growth temperature was 55 ℃ and the optimum growth pH value 8.0. Under optimum growth condition, the maximum NH4+ unit volume reduction rates, NC8 and NJ26 were 1 033.3 mg/L and 1 183.67 mg/L, respectively. Moreover, maximum NO2- and NO3- unit volume production rates of these strains were 23.79 mg/L, 272.30 mg/L and 33.81 mg/L, 366.70 mg/L, respectively. All results demonstrated that strains NC8 and NJ26 are heterotrophic ammonia-oxidizing bacteria which can oxidize NH4+ into NO2- and NO3-, with NO3- as the main product. These bacteria are expected to be applied in reducing ammonia emissions and increasing nitrogen content in compost.

参考文献/References:

1 郭建华, 彭永臻. 异养硝化、厌氧氨氧化及古菌氨氧化与新的氮循环[J]. 环境科学学报, 2008, 28 (8): 1489-1498 [Guo JH, Peng YZ. Heterotrophic nitrification, anaerobic ammonia oxidation and archaeal ammonia oxidation in a new nitrogen cycle [J]. Acta Sci Circumst, 2008, 28 (8): 1489-1498]
2 贺纪正, 张丽梅. 氨氧化微生物生态学与氮循环研究进展[J]. 生态学报, 2009, 29 (1): 406-415 [He JZ, Zhang LM. Advances in ammonia-oxidizing microorganisms and global nitrogen cycle [J]. Acta Ecol Sin, 2009, 29 (1): 406-415]
3 Prosser JI. Autotrophic nitrification in bacteria [M]. Adv Microb Physiol, 1989, 30: 125-181
4 Kraft B, Strous M, Tegetmryer HE. Microbial nitrate respiration - Genes, enzymes and environmental distribution [J]. J Biotechnol, 2011, 155 (1): 104-117
5 Kowalchuk GA, Stephen JR, Ammonia-oxidizing bacteria: a model for molecular microbial ecology [J]. Annu Rev Microbiol, 2001, 55: 485-529
6 Chikako S, Tomoyoshi H. Isolation and characterization of novel thermophilic nitrifying Bacillus sp. from compost [J]. Soil Sci Plant Nutr, 2011, 57 (1): 150-156
7 刘志培, 贾省芬, 俞志明, 刘双江. 新型异养氨氧化菌的分离鉴定及氨氧化特性[J]. 环境污染与防治, 2005, 27 (5): 337-339
8 Daum M, Zimmer W, Papen H, Kloos K, Bothe H. Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida [J]. Curr Microbiol, 1998, 37 (4): 281-288
9 Moir JWB, Crossman LC, Spiro S, Richardson DJ. The purification of ammonia monooxygenase from Paracoccus denificans [J]. FEBS Lett, 1996, 387 (1): 71-74
10 Anderson IC, Poth M, Homstead J, Burdige D. A comparison of NO and N2O production by the antotrophic nitrifier Nitorsomonas europaea and the heterotrophic nitrifier Alcaligenes faecalis [J]. Appl Environ Microbiol, 1993, 59 (11): 3525-3533
11 Robertson AL, Niel EWJ, Torremans RAM, Kuenen JG. Simultaneus nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha [J]. Appl Environ Microobiol, 1988, 54 (11): 2812-2818
12 Chikako S. Tomoyoshi H. Improvement of media for thermophilic ammonia-oxidizing bacteria in compost [J]. Soil Sci Plant Nutr, 2008, 54 (4): 529-533
13 Maeda K, Hanajima D, Toyoda S, Yoshida N, Morioka R, Osada T. Microbiology of nitrogen cycle in animal manure compost [J]. Microb Biotechnol, 2011, 4 (6): 700-709
14 Robb FT. Thermophiles Archaea: A laboratory manual [M]. Woodbury: Cold Spring Harbor, 1995. 173
15 刘志培, 刘双江, 硝化作用微生物的分子生物学研究进展[J]. 应用与环境生物学报, 2004, 10 (4): 521-525 [Liu ZP, Liu SJ. Advances in the molecular biology of nitrifying microorganisms [J]. Chin J Appl Environ Biol, 2004, 10 (4): 521-525]
16 Coorevits A, Logan NA, Dinsdale AE, Halket G, Scheldeman P, Heyndrickx M, Schumann P, Landschoot AV, Vos PD. Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans [J]. Int J Syst Evol Micr, 2011, 61 (8): 1954-1961
17 Combet-Blanc Y, Ollivier B, Streicher C, Patel BKC, Dwivedi PP, Pot B, Prensier G, Garcia J-L. Bacillus themoamylovorans sp. nov., a moderately thermophilic and amylolytic bacterium [J]. Int J Syst Bacteriol, 1995, 45 (1): 9-16
18 Scheldeman P, Pil A, Herman L, Vos PD, Heyndrickx M. Incidence and diversity of potentially highly heat-resistant spores isolated at dairy farms [J]. Appl Environ Microbiol, 2005, 71 (3): 1480-1494
19 Rainey FA, Fritze D, Stackebrandt E. The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis [J]. FEMS Microbiol Lett, 1994, 115 (2-3): 205-211

备注/Memo

备注/Memo:
国家自然科学基金项目(30960022和31160035)资助
更新日期/Last Update: 2014-03-06