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[1]彭晓珏,刘瑢,阳菁,等.Gateway技术构建盐和干旱胁迫下水稻根系混合cDNA文库及其质量鉴定[J].应用与环境生物学报,2014,20(02):291-294.[doi:10.3724/SP.J.1145.2014.00291]
 PENG Xiaojue,LIU Rong,YANG Jing,et al.Construction and identification of a mixed cDNA library of rice roots under salt and drought stress using Gateway technology[J].Chinese Journal of Applied & Environmental Biology,2014,20(02):291-294.[doi:10.3724/SP.J.1145.2014.00291]
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Gateway技术构建盐和干旱胁迫下水稻根系混合cDNA文库及其质量鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年02期
页码:
291-294
栏目:
研究简报
出版日期:
2014-04-25

文章信息/Info

Title:
Construction and identification of a mixed cDNA library of rice roots under salt and drought stress using Gateway technology
作者:
彭晓珏刘瑢阳菁丁霞王芳李阳生蔡耀辉
1南昌大学生命科学与食品工程学院/分子生物学与基因工程实验室 南昌 330031 2武汉大学生命科学学院/植物遗传发育学教育部重点实验室 武汉 430072 3杂交水稻国家重点实验 武汉 430072 4江西省超级水稻研究发展中心 南昌 330200
Author(s):
PENG Xiaojue LIU Rong YANG Jing DING Xia WANG Fang LI Yangsheng CAI Yaohui
1 Key laboratory of Molecular Biology and Gene Engineering, School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330031, China 2 Key MOE Laboratory for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China 3 State Key Laboratory of Hybrid Rice, Wuhan 430072, China 4 Jiangxi Super-rice Research and Development Center, Nanchang 330200, China
关键词:
水稻抗逆GatewaycDNA文库盐胁迫干旱胁迫
Keywords:
rice stress tolerance Gateway cDNA library salt stress drought stress
分类号:
Q785 : S511.034
DOI:
10.3724/SP.J.1145.2014.00291
文献标志码:
A
摘要:
为研究水稻非生物胁迫响应信号转导的网络途径,揭示水稻抗逆的分子机制,以NaCl胁迫、干旱胁迫处理及正常生长的水稻根系为材料,采用Gateway技术构建了水稻根系受盐和干旱诱导的混合cDNA文库. cDNA文库质量分析表明,未经扩增的原始文库容量为1.5×106 cfu, 插入片段大小主要为1 kb左右,重组率为100%. 文库随机挑选克隆测序,结果显示插入序列完整性良好,文库中包含水解酶基因、逆境响应蛋白基因、氧化还原酶基因和富含甘氨酸的RNA结合蛋白质基因等. 因此,本研究构建的盐和干旱诱导的水稻根系混合cDNA文库符合高质量文库的标准,可以用于进一步开展相关基因的克隆及功能研究.
Abstract:
In order to study the pathway of rice response to abiotic stresses and discover the molecular mechanisms of stress tolerance in rice, we constructed a mixed cDNA library by using Gateway technology from rice roots subjected to salt and drought treatments. The quality assessment of this library showed that the titer of the unamplified library was 1.5 × 106 cfu, and the average insert size about 1 kb with recombination efficiency of 100%. In this study, randomly-selected clones were sequenced to find many functional genes, including glycosyl hydrolases, stress induced protein, oxidoreductase and glycine-rich RNA-binding protein, etc. The results suggested that the mixed cDNA library from rice roots was successfully generated in high quality, and it may provide an essential resource for further stress-responsive gene cloning and functional study.

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备注/Memo

备注/Memo:
国家科学自然基金项目(31160270,31160019)、教育部科学研究重大项目(313039)和江西省青年科学基金项目(20114BAB-214009)资助
更新日期/Last Update: 2014-05-04