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[1]邢晔,刘艳,张超,等.中国白酒酿造物料中分离的酵母菌株在不对称羰基还原中的应用[J].应用与环境生物学报,2013,19(06):1014-1019.[doi:10.3724/SP.J.1145.2013.01014]
 XING Ye,LIU Yan,ZHANG Chao,et al.Asymmetric Ketone Reduction Using Targeted Yeast Collections Isolated from Chinese Liquor Pits[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):1014-1019.[doi:10.3724/SP.J.1145.2013.01014]
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中国白酒酿造物料中分离的酵母菌株在不对称羰基还原中的应用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年06期
页码:
1014-1019
栏目:
研究论文
出版日期:
2013-12-25

文章信息/Info

Title:
Asymmetric Ketone Reduction Using Targeted Yeast Collections Isolated from Chinese Liquor Pits
作者:
邢晔刘艳张超林晖杨涛庄名扬吴中柳
(1中国科学院成都生物研究所,中国科学院环境与应用微生物重点实验室 成都 610041) (2中国科学院成都生物研究所,环境微生物四川省重点实验室 成都 610041) (3中国科学院大学 北京 100049)
Author(s):
XING Ye LIU Yan ZHANG Chao2 LIN Hui1 2 YANG Tao1 2 ZHUANG Mingyang1 2 & WU Zhongliu
(1Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (2Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (3University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
羰基还原酶菌种筛选酵母手性醇中国白酒
Keywords:
ketone reductase strain screening yeast chiral alcohol Chinese liquor
分类号:
TS261.1 : O621.3
DOI:
10.3724/SP.J.1145.2013.01014
文献标志码:
A
摘要:
中国白酒窖的酿造物料中含有大量的细菌、酵母及霉菌菌株. 为了研究其中的代表性酵母菌株在不对称羰基还原中的应用,利用5种不同的羰基化合物为底物,对保存于中国科学院成都生物研究所中国白酒酿造微生物菌种库中的部分酵母菌株进行了筛选. 结果表明大多数菌株都具有羰基还原酶活力,绝大多数符合Prelog规则,其中部分菌株还具有优秀的立体选择性,在药物手性中间体合成应用中具有一定的应用潜力:例如其中拟威尔酵母属Williopsis sp. 2.045在转化底物3,5-双三氟甲基苯乙酮时的转化率以及S构型产物光学纯度均高于99%;红酵母属Rhodotorula sp. 2.154在转化底物N-甲基-3-羰基-3-(2-噻吩)丙酰胺时的转化率及S构型产物光学纯度达到了95%和99%. 这些发现也表明白酒酿造物料中含有大量具有羰基还原酶活力的生物资源,因此中国白酒酿造微生物菌种库可作为不对称羰基还原研究中的储备酶库,直接对目标库中的菌种进行筛选能够大幅度提高菌株选择的效率.
Abstract:
Plenty of bacteria, yeasts, molds have been found in Chinese liquor pits. In order to investigate the applications of specific yeasts on ketone reduction, we screened some yeast strains in the Yeast Library of Chinese Liquor Pits in Chengdu Institute of Biology, Chinese Academy of Sciences, using five carbonyl compounds as potential substrates. The results indicated that the majority of the strains were capable of ketone reduction, and most of them obeyed the Prelog’s rule. Some of the strains showed excellent stereo-selectivity, indicating high application potential in chiral drug intermediates production. For example, Williopsis sp. 2.045 transfered 3,5-bis(trifluoromethyl)-acetophenone into (S)-3,5-bis(trifluoromethyl)-1-phenethanol with over 99% yield and ee; Rhodotorula sp. 2.154 changed N-methyl-3-oxo-3-(thiophen-2-yl)propanamide into (S)-3-hydroxy-N-methyl-3-(thiophen-2-yl)propanamide with a yields of 95% and 99% ee. The results suggested that Chinese liquor pits contains lots of bioresources with high ketone reducing activity. It could be used as a targeted library in the screening of microbes with ketone reductase activity, which can highly improve the efficiency of strain screening.

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备注/Memo

备注/Memo:
国家自然科学基金项目(21072183)和中国科学院“西部之光”基金项目资助
更新日期/Last Update: 2014-01-03