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[1]晋方佑,李治滢,王永霞,等.云南程海湖可培养真菌多样性分析[J].应用与环境生物学报,2013,19(06):1025-1030.[doi:10.3724/SP.J.1145.2013.01025]
 JIN Fangyou,LI Zhiying,WANG Yongxia,et al.Diversity of Cultivable Fungi Isolated from the Chenghai Lake in Yunnan Province[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):1025-1030.[doi:10.3724/SP.J.1145.2013.01025]
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云南程海湖可培养真菌多样性分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年06期
页码:
1025-1030
栏目:
研究论文
出版日期:
2013-12-25

文章信息/Info

Title:
Diversity of Cultivable Fungi Isolated from the Chenghai Lake in Yunnan Province
作者:
晋方佑李治滢王永霞董明华严亚萍李绍兰杨丽源
(云南大学云南省微生物研究所 昆明 650091)
Author(s):
JIN Fangyou LI Zhiying WANG Yongxia DONG Minghua YAN Yaping LI Shaolan YANG Liyuan
(Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China)
关键词:
高原湖泊淡水真菌土壤真菌多样性相关性分析
Keywords:
plateau lake freshwater fungi soil fungi diversity correlation analysis
分类号:
Q949.320.8 (274)
DOI:
10.3724/SP.J.1145.2013.01025
文献标志码:
A
摘要:
对具有特殊环境的高原湖泊程海及其周边土壤中真菌的多样性及生态分布进行研究,并分析湖水理化因子与真菌数量的关系. 用经典形态学方法鉴定真菌,并分析不同生境的真菌多样性、均匀度及相似性;用软件IBM SPSS Statistics 19对冬季湖水8个样点的理化因子与真菌数量进行Pearson相关性分析和显著性检验,分析湖水理化因子对真菌数量的影响. 分离到的1 760株真菌中,有1 504株产孢,鉴定为98个属,优势属为曲霉属(Aspergillus)和青霉属(Penicillium),分离频率分别为14.89%和12.39%. 多样性指数和均匀度指数分析表明,春季土壤和冬季底泥中真菌的多样性最高,其Shannon-Wiener指数和Simpson指数分别为2.917 7、0.907 4和2.910 7、0.918 5,而冬季湖水的均匀度最高,为0.793 1. 相似性系数显示,冬季湖水和冬季底泥的真菌菌群相似性最高,它们之间的Jaccard指数和Sorenson指数分别为0.365 4和0.535 2. Pearson相关性分析发现,温度、总氮、总硬度与真菌数量呈正相关;pH、电导率、浊度、总磷、总有机碳与真菌数量呈负相关;显著性检验显示,这8项理化因子与真菌数量的相关性均不显著. 本研究表明程海湖存在大量的真菌资源,并具有丰富的真菌多样性,为程海真菌的活性筛选和代谢产物研究奠定了基础.
Abstract:
The aims of this study were to investigate the fungal diversity and the ecological distribution of these fungi in the plateau lake and surrounding soil, to analyze the relations between fungi quality and the physical and chemical factors of lake water. Fungi were identified with classical morphological method. In Addition, fungal diversity, evenness and similarity in different habitats were analyzed. Software IBM SPSS Statistics 19 was used to carry out Pearson correlation analysis and significance test on the physical and chemical factors as well as fungi quantity from 8 winter water samples. Among the total 1 760 strains isolated, 1 504 sporogenous strains were identified as 98 genera. The dominant genera were Aspergillus and Penicillium, with isolation frequency 14.89% and 12.39%, respectively. Both fungal species (65 genera) and isolation frequency (60.13%) were the highest in the spring soil. The analysis of diversity and evenness index showed that fungi in the spring soil and the winter sediment had the highest diversity, with Shannon-Wiener index and Simpson index as 2.917 7, 0.907 4 and 2.910 7, 0.918 5, respectively; the evenness index of winter lake water was the largest (0.793 1). Similarity coefficient revealed that fungal similarity between winter lake water and winter sediment was the highest, with Jaccard index and Sorenson index 0.365 4 and 0.535 2, separately. Pearson correlation analysis showed that temperature, total nitrogen, total hardness were positively correlated with fungi quantity, whereas pH, conductivity, turbidity, total phosphorus, and total organic carbon were negatively correlated with fungi quantity. Significance test showed that eight physical and chemical factors were not significantly related to fungi quantity. The investigation suggested that a large number of fungi exist in great diversity in the Chenghai Lake and surrounding soil. The results laid the foundation for subsequent activity screening and metabolic product research.

参考文献/References:

1 Thomas K. Australian freshwater fungi. In: Introductory Volume to the Fungi (Part 2) [M]. Canberra: ABRS, 1996, 1B: 1-37 2 Cai L, Hyde KD, Tsui CK. Genera of freshwater fungi[J]. Fungal Div Res Ser, 2006, 18: 1-261 3 Wong MK, Goh TK, Hodgkiss IJ, Hyde KD, Ranghoo VM, Tsui KM, Ho WH, Wong SW, Yuan TK. Role of fungi in freshwater ecosystems [J]. Biodiv Conserv, 1998 (7): 1187-1206 4 周秀华. 南湖公园土壤真菌群落的初步研究[J]. 长春大学学报, 2010, 20 (2): 26-28 [Zhou XH. Preliminary research of soil fungi community in South lake park [J]. J Changchun Univ, 2010, 20 (2): 26-28] 5 周新丽, 李治滢, 杨丽源, 董明华, 李绍兰. 云南程海湖酵母菌多样性及应用[J]. 微生物学报, 2011, 51 (4): 547-553[Zhou XL, Li ZY, Yang LY, Dong MH, Li SL. Identification of yeasts isolated from Chenghai Lake, a plateau lake in Yunnan province [J]. Acta Microbiol Sin, 2011, 51 (4): 547-553] 6 董云仙, 胡锦乾, 关兆国, 和熊祥, 孔德平, 张淑霞, 周虹霞. 程海冬季水质和富营养化评价[J]. 环境科学导刊, 2010, 29 (4): 75-79 [Dong YX, Hu JQ, Guan ZG, He XX, Kong DP, Zhang SX, Zhou HX. Characteristics and origin and counter measures of water bloom of Chenghai Lake in the winter [J]. Environ Sci Surv, 2010, 29 (4): 75-79] 7 彭小伟, 杨丽源, 周斌, 李治滢, 李绍兰, 陈有为. 植物黄花夹竹桃内生真菌多样性研究[J]. 云南大学学报(自然科学版), 2003, 25 (增刊): 28-31 [Peng XW, Yang LY, Zhou B, Li ZY, Li SL, Chen YW. Study on the diversity of endophytic fungi in Thevetia peruviana [J]. J Yunnan Univ, 2003, 25 (Suppl.): 28-31] 8 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979 [Wei JC. Fungal Identification Manual [M]. Shanghai: Shanghai Science and Technology Press, 1979] 9 巴尼特 HL, 亨特 BB著. 半知菌属图解[M]. 沈崇尧译. 北京: 科学出版社, 1977 [Barnett HL, Hunter BB. Illustrated Genera of Imperfect Fungi [M]. Translated by Shen CY. Beijing: Science press, 1977] 10 于晶, 周峰, 陈君, 王霞, 徐江, 刘同宁. 肉苁蓉内生真菌多样性研究[J]. 中国中药杂志, 2011, 36 (5): 542-546 [Yu J, Zhou F, Chen J, Wang X, Xu J, Liu TN. Diversity of endophytic fungi from Cistanche deserticola [J]. China J Chin Mater Med, 2011, 36 (5): 542-546] 11 杜少康, 陈双林, 林岱, 吴鸣谦, 王梅霞. 银杏叶部内生真菌多样性的研究[J]. 菌物学报, 2009, 28 (4): 504-511 [Du SK, Chen SL, Lin D, Wu MQ, Wang MX. Diversity of endophytic fungi in leaves of Ginkgo biloba [J]. Myco, 2009, 28 (4): 504-511] 12 杨发蓉, 杨瑞华, 丁骅孙, 周应揆. 滇池水体中水生真菌类群分布的研究[J]. 云南大学学报, 1985, 7 (增刊): 85-93 [Yang FR, Yang RH, Ding HS, Zhou YK. The distribution of the aquatic fungi groups in the water of Yunnan Dian-chi lake [J]. J Yunnan Univ, 1985, 7 (Suppl.): 85-93] 13 杨发蓉, 丁骅孙. 洱海湖体真菌类群分布的研究[J]. 云南大学学报, 1986, 8 (3): 319-324 [Yang FR, Ding HS. A study distribution of the fungi groups in the limnetic body of Er-hai lake[J]. J Yunnan Univ, 1986, 8 (3): 319-324] 14 Yang FR. Distribution study of aquatic fungi in Changhu Lake from Yunnan Province [J]. J Yunnan Univ (Nat Sci), 1992, 14 (2): 233-237 15 宋瑞清, 吴雪梅. 乌伊岭湿地自然保护区水体淡水真菌多样性研究[J]. 沈阳农业大学学报, 2010, 41 (3): 289-293 [Song RQ, Wu XM. Preliminary studies on diversity of freshwater fungi in Wuyiling Wetland Nature Reserve [J]. J Shenyang Agric Univ, 2010, 41 (3): 289-293] 16 刘晓娟, 朱旭东, 余永年. 北京地区水泡中的丝孢菌及其生态[J]. 真菌学报, 1992, 11 (2): 111-118 [Liu XJ, Zhu XD, Yu YN. Aquatic hyphomycetes in water foam from Beijing region [J]. Atca Mycol Sin, 1992, 11 (2): 111-118] 17 金静, 王远路, 刘建萍. 砚河水域水生真菌的分离[J]. 当代生态农业, 2004, 10 (1): 34-36 [Jin J, Wang YL, Liu JP. Separation of aquatic fungi in waters of Inkstone river[J]. Contemp Eco-Agri, 2004, 10 (1): 34-36] 18 Luo J, Yin JF, Cai L, Zhang KQ, Hyde KD . Freshwater fungi in Lake Dianchi, a heavily polluted lake in Yunnan, China [J]. Fungal Div, 2004, 23 (1): 7-13 19 文航, 蔡佳亮, 苏玉, 王东伟, 黄乙. 滇池流域入湖河流丰水期着生藻类群落特征及其与水环境因子的关系[J] .湖泊科学, 2011, 23 (1): 40-48 [Wen H, Cai JL, Su Y, Wang DW, Huang Y. Characteristics of periphytic algal community and its relationship with aquatic environmental factors in streams of Lake Dianchi watershed in wet season [J]. J Lake Sci, 201l, 23 (1): 40-48] 20 宣淮翔, 安树青, 孙庆业, 周长芳. 太湖不同湖区水生真菌多样性[J]. 湖泊科学, 2011, 23 (3): 469-478 [Xuan HX, An SQ, Sun QY, Zhou CF. Diversity of aquatic fungi in different areas of Lake Taihu. J Lake Sci, 2011, 23 (3): 469-478] 21 Somova LA, Pechurkin NS. Functional, regulatory and indicator features of microorganisms in man- made ecosystems [J]. Adv Space Res, 2001, 27 ( 9): 1563- 1570 22 张晶, 张惠文, 李新宇, 张成刚. 土壤真菌多样性及分子生态学研究进展[J]. 应用生态学报, 2004, 15 (10): 1958-1962 [Zhang J, Zhang HW, Li XY, Zhang CG. Research advances in soil fungal diversity and molecular ecology [J]. Chin J Appl Ecol, 2004 , 15 (10): 1958-1962] 23 Luo J, Zhang KQ, Cai L, Kevin Hyde. Freshwater fungi in Mainland China [J]. J Huazhong Agric Univ, 2004, 23 (1): 7-13

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备注/Memo

备注/Memo:
国家自然科学基金项目(31160006)、云南省科技条件平台建设计划项目(2009DA002)和云南省教育厅科学研究基金项目(09Z0006)资助
更新日期/Last Update: 2014-01-03