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[1]杨旭,许科伟,刘和,等.油气藏上方土壤中甲烷氧化菌群落结构分析——以沾化凹陷某油气田为例[J].应用与环境生物学报,2013,19(03):478-483.[doi:10.3724/SP.J.1145.2013.00478]
　YANG Xu,XU Kewei,LIU He,et al.Analysis of Methane-oxidizing Bacteria Community Structure in the Soil above Oil and Gas Reservoirs - An Oil and Gas Field of Zhanhua Depression as an Example[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):478-483.[doi:10.3724/SP.J.1145.2013.00478]
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油气藏上方土壤中甲烷氧化菌群落结构分析——以沾化凹陷某油气田为例()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年03期
页码:
478-483
栏目:
研究论文
出版日期:
2013-06-25

文章信息/Info

Title:
Analysis of Methane-oxidizing Bacteria Community Structure in the Soil above Oil and Gas Reservoirs - An Oil and Gas Field of Zhanhua Depression as an Example
作者:
杨旭; 许科伟; 刘和; 任春; 汤玉平; 黄欣
(1江南大学环境与土木工程学院 无锡 214122)
(2中国石化石油勘探开发研究院无锡石油地质研究所 无锡 214151)
Author(s):
YANG Xu; XU Kewei; LIU He; REN Chun; TANG Yuping; HUANG Xin
(1School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China)
(2Wuxi Research Institute of Petroleum Geology, Exploration and Production Research Institute, SINOPEC, Wuxi 214122, China)
关键词:
甲烷氧化菌; pmoA基因; 克隆文库; 群落结构; 油气微生物勘探; 胜利油田
Keywords:
methane oxidizing bacteria;  pmoA;  clone library;  community structure;  oil and gas microbial exploration;  Shengli Oil Field
分类号:
Q939.99 : P618.130.8
DOI:
10.3724/SP.J.1145.2013.00478
摘要:
以胜利油田沾化凹陷某油气田内油区、气区和背景区上方近地表的土壤为例，构建了甲烷氧化菌功能基因（甲烷单加氧酶基因）pmoA的克隆文库，以探讨甲烷氧化菌群落结构与地下油气藏之间的关系. 对3个文库155个克隆子测序后进行比较分析，发现Ⅰa型甲烷氧化菌Methylobacter、Methylomonas、Methylomicrobium在油区土壤中所占比例分别为23%、21%和17%，在气区分别为31%、15%和16%，在背景区分别为26%、21%和8%；Ⅰb型甲烷氧化菌Methylococcus和Methylocaldum在3个区的比例分别为29%、31%和19%；Ⅱ型甲烷氧化菌Methylocystis和Methylosinus在油区所占比例为10%，在气区为7%，在背景区为27%. Ⅰa型甲烷氧化菌在各区域分布差别不明显，Ⅰb型甲烷氧化菌Methylococcus和Methylocaldum在油气区的丰度较高，而Ⅱ型甲烷氧化菌Methylocystis和Methylosinus在背景区的丰度远远高于油气区. 由此可知，在地质历史时期的持续轻烃供应下，长期的油气微渗漏环境可能会促使微生物群落结构产生差异，Ⅱ型到Ⅰ型甲烷氧化菌缓慢地演替.
Abstract:
In order to explore the relationship between the methane oxidizing bacterial community and the oil and gas reservoir beneath the soil, samples collected from near-surface soil in oil, gas, and reference areas around Zhanhua oil and gas field were investigated by constructing three clone libraries of pmoA, a functional gene for methane monooxygenase in methane oxidizing bacteria. All together 155 picked clones from the three libraries were phylogenetically analyzed, and the result showed that Methylobacter, Methylomonas, Methylomicrobium associated with the type Ⅰa methanotroph represented 23%, 21% and 17% in the oil area, 31%, 15%, 16% in the gas area, and 26%, 21%, 8% in the reference area respectively; Methylococcus and Methylocaldum associated with the type Ⅰb methanotroph represented 29%, 31%, 19% in the oil, gas and reference area respectively; Methylocystis and Methylosinus accounted for 10%, 7%, 27% in the oil, gas and reference area respectively. Therefore, the distribution difference of type Ⅰa methanotroph in different areas was not significant, but the distribution of type Ⅰb and type Ⅱ was different in the three different areas. The oil and gas area showed a higher abundance of type Ⅰb methanotroph Methylococcus and Methylocaldum than the reference area, while a lower abundance of type Ⅱ methanotrophs Methylocystis and Methylosinus. It was thus concluded that the geological history of sustained light hydrocarbon supply and the long-term oil, gas micro-seepage might cause different microbial community structures and gradual succession from type Ⅱ to type I in the methanotroph community.

参考文献/References:

Abrams MA. Distribution of subsurface hydrocarbon seepage in near-surface marine sediments [J]. AAPG Memoir, 1996, 66: 1-14
Hitzman DO. Prospecting for petroleum deposits [P]. USA, No. 2880142, 1959-03-31
向延生, 周俊初, 袁志华. 利用地表甲烷氧化菌异常勘探天然气藏[J]. 天然气工业, 2005, 23: 41-43
Hanson RS. Methanotrophic bacteria [J]. Microbiol Rev, 1996, 60: 439-471
Stoecker K, Bendinger B, Schoning B. Cohn’s Crenothrix is a filamentous methane oxidizer with an unusual methane monooxygenase [J]. Proc Nat Acad Sci USA, 2006, 103: 2363-2367
Martineau C, Whyte LG, Greer CW. Stable isotope probing analysis of the diversity and activity of methanotrophic bacteria in Soils from the Canadian High Arctic [J]. Appl Environ Microbiol, 2010, 76: 5773-5784
Han B, Chen Y, Abell G, Jiang H, Bodrossy L, Zhao J, Murrell JC, Xing XH. Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine [J]. FEMS Microbiol Ecol, 2009, 70: 196-207
Zhang F, She Y, Zheng Y, Zhou Z, Kong S, Hou D. Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China [J]. Appl Microbiol Biot, 2010, 86: 1183-1194
Murrell JC, Gillbert B, McDonald IR. Molecular biology and regulation of methane monooxygease [J]. Arch Microbiol, 2000, 173: 325-332
Kolb S, Knief C, Stubner S. Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays [J]. Appl Environ Microbiol, 2003, 69: 2423-24291
Kemp P, Aller J. Estimating prokaryotic diversity: when are 16S rDNA libraries large enough [J]. Limnol Oceanogr-Meth, 2004, 2: 114-125
Bowman JP, McCammon SA. Methylosphaera hansonii gen. nov.,a psychrophilic, group 1 methanotroph from Antarctic marine-salinity, meromictic lakes [J]. Microbiology, 1997, 143: 1451-1459
Dedysh SN, Derakshani M. Detection and enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA fluorescence in situ hybridization, including the use of a newly developed oligonucleotide probe for Methylocella palustris [J]. Appl Environ Microbiol, 2001, 67: 4850-4857
Bodrossy L, Holmes EM. Analysis of 16S rRNA and methane monooxygenase gene sequences reveals a novel group of thermotolerant and thermophilic methanotrophs, Methylocaldum gen. nov. [J]. Arch Microbiol, 1997, 168: 493-503
Bowman DG, Sly LI. The phylogenetic position of the family Methylococcaceae [J]. Int J Syst Bacteriol, 1995, 45: 182-185
Whittenbury R, Phillips KC. Enrichment, isolation and some properties of methane utilizing bacteria [J]. J Gen Microbial, 1970, 61: 205-218
Kim JS, Sparorek G, Longe RM, DeMelo WJ, Crowley D. Bacterial diversity of terra preta and pristine forest soil from the Western Amazon [J]. Soil Biol Biochem, 2007, 39: 684-690
黄祖新, 黄镇, 叶冰莹, 陈由强, 陈如凯. 宿根甘蔗根际土壤细菌多样性分析中培养法与非培养法比较研究[J]. 应用与环境生物学报, 2011, 17 (5): 742-746 [Huang ZX, Huang Z, Ye BY, Chen YQ, Chen RK. Comparison of culture-dependent and -independent approaches for diversity analysis of soil bacterial in the rhizosphere of sugarcane [J]. Chin J Appl Environ Biol, 2011, 17 (5): 742-746]
冯晓虎, 朱卫华, 吴弘, 谢洁, 张书, 巨艳, 乔代蓉, 曹毅. 若尔盖高原湿地纤维素降解菌组成及产酶特性[J]. 应用与环境生物学报, 2010, 16 (3): 399-402 [Feng XH, Zhu WH, Wu H, Xie J, Zhang S, Ju Y, Qiao DR, Cao Y. Compositon and enzyme-producting property of cellulolytic microbes in high altitude wetland of Zoige, China [J]. Chin J Appl Environ Biol, 2010, 16 (3): 399-402]
Zheng Y, Zhang LM, Zheng YM, Di HJ, He JZ. Abundance and community composition of methanotrophs in a Chinese paddy soil under long-term fertilization parctices [J]. J Soils Sediment, 2008, 8: 406-414
Deutzmann JS, Wörner S, Schink B. Activity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments [J]. Appl Environ Microbiol, 2011, 77 (8): 2573-2581
Kallistova AY, Kevbrina MV, Nekrasova VK, Shnyrev NA, Einola JM, Kulomaa MS, Rintala JA, Nozhevnikova AN. Enumeration of methanotrophic bacteria in the cover soil of an aged municipal landfill [J]. Microbial Ecol, 2007, 54: 637-645
Hanson RS. Methanotrophic bacteria [J]. Microbiol Rev, 1996, 60: 439-471
Kolb S. The quest for atmospheric methane oxidizers in forest soils [J]. Env Microbiol Rep, 2009, 1: 336-346
Dunfield PF, Liesack W, Henckel T, Knowles R, Conrad R. High-affinity methane oxidation by a soil enrichment culture containing a type Ⅱ methanotroph [J]. Appl Environ Microbiol, 1999, 65: 1009-1014
Andrews J, Harris R. R-selection and K-selection and microbial ecology. Adv Microb Ecol, 1986, 9: 99-147
Krause S, Lüke C, Frenzel P. Succession of methanotrophs in oxygen-methane counter-gradients of flooded rice paddies [J]. ISME J, 2010, 4: 1603-1607
Boeckx P, Cleemput OV. Methane oxidation in a neutral landfill cover soil: influence of moisture content, temperature, and nitrogen-turnover [J]. J Environ Qual, 1996, 25: 178-183
Valentine DL. Emerging topics in marine methane biogeochemistry [J]. Annu Rev Mater Res, 2011, 3: 147-171
Striegl RG, McConnaughey TA, Thorstenson DC, Weeks EP, Woodward JC. Consumption of atmospheric methane by desert soils [J]. Nature, 1992, 357: 145-147
汤玉平, 丁相玉, 吴向华, 魏巍, 李尚刚. 中国主要含油气盆地区域地球化学场参数特征及其成因研究[J]. 石油勘探与开发, 2001, 28 (3) : 1-4 [Tang YP, Ding XY, Wu XH, Wei W, Li SG. Features and genesis of regional geochemical fields of the main hydrocarbon-bearing basins in China [J]. Petrol Explor Dev, 2001, 28 (3) : 1-4]

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备注/Memo

备注/Memo:
中央高校基本科研业务费专项资金（JUSRP31105，JUSRP111A10）资助
更新日期/Last Update: 2013-06-20