|本期目录/Table of Contents|

[1]李明,郭嘉,石正国,等.春季青藏高原东北部湖泊细菌种类组成[J].应用与环境生物学报,2013,19(05):750-758.[doi:10.3724/SP.J.1145.2013.00750]
 LI Ming,GUO Jia,SHI Zhengguo,et al.Bacterial Community Structure in Lakes on the Northeastern Qinghai-Tibetan Plateau[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):750-758.[doi:10.3724/SP.J.1145.2013.00750]
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春季青藏高原东北部湖泊细菌种类组成()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年05期
页码:
750-758
栏目:
研究论文
出版日期:
2013-10-25

文章信息/Info

Title:
Bacterial Community Structure in Lakes on the Northeastern Qinghai-Tibetan Plateau
作者:
李明郭嘉石正国晏利斌晏宏
(1中国科学院地球环境研究所黄土与第四纪地质国家重点实验室 西安 710075) (2西安交通大学医学院第一附属医院放疗科 西安 710061)
Author(s):
LI Ming GUO Jia SHI Zhengguo YAN Libin YAN Hong
(1State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710075, China) (2Department of Radiotherapy, First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, China)
关键词:
青藏高原湖泊细菌多样性变形菌纲分类单元
Keywords:
the Qinghai-Tibet Plateau lake bacterial biodiversity Proteobacteria operational taxonomic unit
分类号:
Q938.1 : Q178.53
DOI:
10.3724/SP.J.1145.2013.00750
摘要:
青藏高原东北地区位于我国东部季风区、西北干旱区和青藏高原高寒区的交汇地带,人为影响较少,是研究人为影响与生物种群组成的理想场所,然而目前尚缺乏大范围的分子生物学研究。2012年4-5月期间对青藏高原东北部10个湖水进行采样调查(表层、底层水各0.5 L混匀),应用16S rRNA和PCR-DGGE指纹分析研究细菌种类和组成。共鉴别出样本湖水中117个分类单元(Operational taxonomic unit,OTU)的细菌,分别隶属于12个纲,其中以α-变形菌纲(21种)和β-变形菌纲(27种)检出频次最多。细菌主要源于水生的微生物,同时各湖区细菌分类单元数量(平均32.1 ± 11.2)偏少,多样性指数(平均0.97 ± 0.29)和均匀度指数(平均0.19 ± 0.04)偏低,湖区之间相似性(平均0.31 ± 0.11)较差,种群生态结构脆弱。各湖区物种分类单元数量受环境因素影响明显,温度与种群分类单元数(R = 0.763, P < 0.05)、多样性指数(R = 0.708, P < 0.05)及均匀度指数(R = 0.641, P < 0.05)正相关,湖水面积与种群分类单元数量(R = 0.754, P < 0.05)及多样性系数(R = 0.668, P < 0.05)呈正相关,并且细菌种类组成受到湖水水化学(如矿化度等)因素影响。本研究表明春季青藏高原东北部10个湖区细菌种类组成较为简单,生态结构脆弱,受自然环境因素限制明显,结果可为青藏高原东北部地区水生生物研究积累基础资料,亦可为群落结构的限制性气候因素分析、区域的可持续利用和保护提供科学依据。
Abstract:
The northeast of Qinghai-Tibetan Plateau is located in the convergence zone of the monsoon region of eastern China, northwest arid area and Qinghai-Tibetan Plateau region. During April to May 2012, an investigation was carried out on the bacterial community structure in ten lakes on northeastern Qinghai-Tibetan Plateau. With 16S rRNA sequencing and PCR-DGGE technology, twelve bacteria genus level libraries were constructed with a total of 117 operational taxonomic unit including 27 Betaproteobacteria, 8 Gamaproteobacteria, 21 Alphaproteobacteria, 7 Deltaproteobacteria,16 Acidobacteria, 2 Deinococci, 5 Sphingobacteria, 6 Flavobacteria, 5 Nitrospira, 11 Actinobacteria, 6 Gemmatimonadetes and 3 unclassified bacteria. Most sequences in these studied lakes were closer to aquatic bacteria than any other bacterial clusters. The number of operational taxonomic unit in northeastern Qinghai-Tibetan Plateau was 32.1 ± 11.2, Shannon-Wiener diversity index 0.97 ± 0.29, Pielou’s evenness index 0.19 ± 0.04, and Sorenson similarity index between lakes 0.31 ± 0.11, all comparatively low. Notably, the environment factors played an important role in bacteria community, with temperature positively related to OTUs (R = 0.763, P < 0.05), to Shannon-Wiener diversity index (R = 0.708, P < 0.05), and to Pielou’s evenness index (R = 0.641, P < 0.05); the lake area was also positively related to OTUs (R= 0.754, P < 0.05) and Shannon-Wiener diversity index (R = 0.668, P < 0.05). Water chemistry also played an important part in bacteria community. The result is helpful for study of climatic factors of community structure and sustainable development in this region.

参考文献/References:

1 Asao M, Pinkart HC, Madigan MT. Diversity of extremophilic purple phototrophic bacteria in Soap Lake, a Central Washington (USA) Soda Lake [J]. Environ Microbiol, 2011, 13 (8): 2146-2157 2 Muyzer G, Dewaal EC, Uitterlinden AG. Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16S ribosomal-RNA [J]. Appl Environ Microb, 1993, 59 (3): 695-700 3 Neuhauser C, Andow DA, Heimpel GE, May G, Shaw RG, Wagenius S. Community genetics: Expanding the synthesis of ecology and genetics [J]. Ecology, 2003, 84 (3): 545-558 4 Whitham TG, Bailey JK, Schweitzer JA, Shuster SM, Bangert RK, Leroy CJ, Lonsdorf EV, Allan GJ, DiFazio SP, Potts BM, Fischer DG, Gehring CA, Lindroth RL, Marks JC, Hart SC, Wimp GM, Wooley SC. A framework for community and ecosystem genetics: from genes to ecosystems [J]. Nat Rev Genet, 2006, 7 (7): 510-523 5 Yan QY, Yu YH, Feng WS, Zhang WJ. DNA polymorphism of the plankton community and its relationships to species composition in the Three Gorges Reservoir Region of the Yangtze River [J]. J Freshwater Ecol, 2006, 21 (3): 385-390 6 Yan QY, Yu YH, Feng WS. Genetic fingerprinting of plankton community provides new insights into aquatic ecology [J]. Prog Nat Sci, 2006, 16 (9): 893-898 7 Jiang HC, Dong HL, Deng SC, Yu BS, Huang QY, Wu QL. Response of archaeal community structure to environmental changes in lakes on the Tibetan Plateau, northwestern China [J]. Geomicrobiol J, 2009, 26 (4): 289-297 8 Felip M, Pace ML, Cole JJ. Regulation of planktonic bacterial growth rates: The effects of temperature and resources [J]. Microbial Ecol, 1996, 31 (1): 15-28 9 Jiang HC, Deng SC, Huang QY, Dong HL, Yu BS. Response of aerobic anoxygenic phototrophic bacterial diversity to environment conditions in saline lakes and Daotang River on the Tibetan Plateau, NW China [J]. Geomicrobiol J, 2010, 27 (5): 400-408 10 An ZS, Colman SM, Zhou WJ, Li XQ, Brown ET, Jull AJT, Cai YJ, Huang YS, Lu XF, Chang H, Song YG, Sun YB, Xu H, Liu WG, Jin ZD, Liu XD, Cheng P, Liu Y, Ai L, Li XZ, Liu XJ, Yan LB, Shi ZG, Wang XL, Wu F, Qiang XK, Dong JB, Lu FY, Xu XW. Interplay between the Westerlies and Asian monsoon recorded in Lake Qinghai sediments since 32 ka [J]. Sci Rep-Uk, 2012, 2: 619 11 魏复盛, 齐文启, 孙宗光. 地表水环境质量标准(GB3838-2002) [S]. 北京: 国家环境保护局, 2002 [Wei FS, Qi WQ, Sun CG. Surface water quality standards [S]. Beijing: National Environmental Protection Agency, 2002] 12 Yan QY, Yu YH, Feng WS, Deng WN, Song XH. Genetic diversity of plankton community as depicted by PCR-DGGE fingerprinting and its relation to morphological composition and environmental factors in Lake Donghu [J]. Microb Ecol, 2007, 54 (2): 290-297 13 Jiang HC, Dong HL, Yu BS, Liu XQ, Li YL, Ji SS, Zhang CLL. Microbial response to salinity change in Lake Chaka, a hypersaline lake on Tibetan Plateau [J]. Environ Microbiol, 2007, 9 (10): 2603-2621 14 Wu QL, Xing P, Liu WT. East Tibetan Lakes harbour novel clusters of Picocyanobacteria as inferred from the 16S-23S rRNA internal transcribed spacer sequences [J]. Microb Ecol, 2010, 59 (3): 614-622 15 David R. Boone RWC. Bergey’s Manual of Systematic Bacteriology [M]. Williams & Wilkins, 1984 16 Wen Z, Zheng MP, Xu XZ, Liu XF, Guo GL, He ZH. Biological and ecological features of saline lakes in northern Tibet, China [J]. Hydrobiologia, 2005, 541: 189-203 17 Liu YQ, Yao TD, Jiao NZ, Liu XB, Kang SC, Luo TW. Seasonal dynamics of the bacterial community in Lake Namco, the largest Tibetan lake [J]. Geomicrobiol J, 2013, 30 (1): 17-28 18 Eiler A, Bertilsson S. Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes [J]. Environ Microbiol, 2007, 9 (3): 838-841 19 Allgaier M, Grossart HP. Diversity and seasonal dynamics of Actinobacteria populations in four lakes in northeastern Germany [J]. Appl Environ Microb, 2006, 72 (5): 3489-3497 20 奚万艳, 吴鑫, 叶文瑾, 杨虹. 太湖梅梁湾水域蓝藻水华前与水华末期细菌群落结构的变化[J]. 应用与环境生物学报, 2007, 1 (1): 97-103 [Xi WY, Wu X, Ye WJ, Yang H. Changes in bacterial community structure during preceding and degraded period of cyanobacterial bloom in a bay of the Taihu Lake [J]. Chin J Applied Environ Biol, 2007,1 (1): 97-103] 21 谢占玲, 王欢, 赵朋, 余静, 吕超, 申贤慧, 龚宝林, 刘英. 分离自青海湖可培养耐盐真菌的多样性研究[J]. 菌物学报, 2012, 2: 187-195 [Xie ZL, Wang H, Zhao P, Yu J, Lv C, Shen HX, Gong BL, Liu Y. Diversity of halotolerant fungi isolated from Qinghai Lake based on culture-dependent investigation [J]. Mycosystema, 2012, 2: 187-195] 22 Burrows S, Elbert W, Lawrence M, P?schl U. Bacteria in the global atmosphere-Part 1: review and synthesis of literature data for different ecosystems [J]. Atmos Chem Phys Discuss, 2009, 9 (3): 10777-10827 23 Sobek A, Olli K, Gustafsson O. On the relative significance of bacteria for the distribution of polychlorinated biphenyls in Arctic ocean surface waters [J]. Environ Sci Technol, 2006, 40 (8): 2586-2593 24 Jiang HC, Dong HL, Zhang GX, Yu BS, Chapman LR, Fields MW. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in Northwestern China [J]. App Environ Microbiol, 2006, 72 (11): 7430-7434 25 Urbach E, Vergin KL, Young L, Morse A, Larson GL, Giovannoni SJ. Unusual bacterioplankton community structure in ultra-oligotrophic Crater Lake [J]. Limnol Oceanogr, 2001, 46 (3): 557-572 26 周敬, 韩凤清, 庞小朋, 罗重光, 闫建平. 黄河源区哈江盐池和苦海(豆错)的地球化学特征研究[J]. 盐湖研究, 18 (3): 18-22 [Zhou J, Han FQ, Pang XP, Luo CG, Yan JP. Preliminary investigation of Hajiang Salt Pond and Kuhai Lake in Yellow River Source Area [J]. J Salt Lake Res, 18 (3): 18-22] 27 Dong H, Zhang G, Jiang H, Yu B, Chapman L, Lucas C, Fields M. Microbial diversity in sediments of saline Qinghai Lake, China: linking geochemical controls to microbial ecology [J]. Microb Ecol, 2006, 51 (1): 65-82 28 Liu XB, Yao TD, Kang SC, Jiao NAZ, Zeng YH, Liu YQ. Bacterial community of the largest oligosaline lake, Namco on the Tibetan Plateau [J]. Geomicrobiol J, 2010, 27 (8): 669-682 29 Jiang HC, Dong HL, Yu BS, Ye Q, Shen J, Rowe H, Zhang CL. Dominance of putative marine benthic Archaea in Qinghai Lake, north-western China [J]. Environ Microbiol, 2008, 10 (9): 2355-2367 30 Huang Y, Zhang ZN, Liu XS, Zhang Y. Studies on the species composition and biodiversity of free-living marine nematodes in the southern Huanghai Sea [J]. Acta Oceanol Sin, 2006, 25 (2): 87-98 31 Aichner B, Herzschuh U, Wilkes H, Vieth A, Bohner J. Delta D values of n-alkanes in Tibetan lake sediments and aquatic macrophytes - A surface sediment study and application to a 16 ka record from Lake Koucha [J]. Org Geochem, 2010, 41 (8): 779-790

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备注/Memo

备注/Memo:
收稿日期 Received: 2013-01-07 接受日期 Accepted: 2013-03-28 *中国科学院知识创新工程重要方向项目(KZZD-EW-04)和科技部国家重大科学研究计划项目(2013CB955904)资助 Supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (No. KZZD-EW-04) and the Major Scientific Research Project of the Ministry of Science and Technology of China (No. 2013CB955904) **通讯作者 Corresponding author (E-mail: liming11@ieecas.cn)
更新日期/Last Update: 2013-10-28