|本期目录/Table of Contents|

[1]张华玲,蔡莹,谭周进,等.超微七味白术散对菌群失调腹泻小鼠肠道微生物代谢多样性的影响[J].应用与环境生物学报,2014,20(01):93-100.[doi:10.3724/SP.J.1145.2014.00093]
 ZHANG Hualing,CAI Ying,TAN Zhoujin,et al.Effects of ultra-micro powder Qiweibaizhusan on metabolism diversity of intestinal microflora in diarrhea mice with dysbacteriosis[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):93-100.[doi:10.3724/SP.J.1145.2014.00093]
点击复制

超微七味白术散对菌群失调腹泻小鼠肠道微生物代谢多样性的影响()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年01期
页码:
93-100
栏目:
研究论文
出版日期:
2014-02-25

文章信息/Info

Title:
Effects of ultra-micro powder Qiweibaizhusan on metabolism diversity of intestinal microflora in diarrhea mice with dysbacteriosis
作者:
张华玲蔡莹谭周进周赛男郭抗萧佘颜蔡光先
1湖南中医药大学2010级研究生班 长沙 410208 2湖南省中药粉体与创新药物省部共建国家重点实验室培育基地 长沙 410208 3湖南中医药大学2011级研究生班 长沙 410208
Author(s):
ZHANG Hualing CAI Ying TAN Zhoujin ZHOU Sainan GUO Kangxiao SHE Yan CAI Guangxian
12010 Postgraduate Courses Class, Hunan University of Chinese Medicine, Changsha 410208, China 2State Key Laboratory and Hunan Breeding Base for Chinese Medicine Powder and Innovative Drugs, Changsha 410208, China 32011 Postgraduate Courses Class, Hunan University of Chinese Medicine, Changsha 410208, China
关键词:
超微中药七味白术散Biolog-Eco代谢多样性肠道微生物菌群失调腹泻
Keywords:
ultra-micro powder of traditional Chinese medicine Qiweibaizhusan Biolog-Eco metabolism diversity intestinal microfloradysbacteriosis diarrhea
分类号:
R285.5
DOI:
10.3724/SP.J.1145.2014.00093
文献标志码:
A
摘要:
为探讨超微七味白术散对菌群失调腹泻疗效的微生态学机理,采用灌胃头孢拉定和硫酸庆大霉素混合抗生素进行小鼠菌群失调腹泻造模,灌胃给药七味白术散传统汤药和1/2剂量超微汤药进行治疗,正常组和模型组灌胃等量的无菌水,3 d后,提取肠道微生物,在有氧条件下,运用Biolog微生物自动分析系统研究肠道微生物的代谢情况. 结果显示,培养72 h时,各组的颜色平均变化率(AWCD)趋于稳定,且正常组>1/2剂量的超微汤药治疗组>传统汤药治疗组>模型组;正常组的Shannon指数、Shannon均匀度、Simpson指数和Mclntosh指数等微生物群落多样性指数均为最大,且与其他各组都存在极显著性差异(P<0.01),1/2剂量超微汤药治疗组的Shannon指数、Simpson指数和传统汤药治疗组的Shannon均匀度、Mclntosh指数分别位于第2位;聚类分析和主成分分析的结果提示抗生素的抑菌作用明显,使得小鼠肠道微生物的某些代谢途径受到影响,七味白术散传统汤药和1/2剂量超微汤药的调控使得小鼠肠道微生物的代谢多样性逐渐恢复平衡,但两种汤药的调控作用存在异同,1/2剂量超微汤药比传统汤药调控作用更显著. 研究表明超微七味白术散对肠道微生物代谢多样性的调控作用显著,对小鼠菌群失调腹泻具有明显的微生态调节作用.
Abstract:
To study the microecological mechanism for ultra-micro Qiweibaizhusan on diarrhea with intestinal dysbacteriosis, the mice were modeled dysbacteriosis by stomach injection of mixed cefradine and gentamycin sulfate. Then the mice in one treatment group were given the traditional decoction of Qiweibaizhusan; those in the other treatment group were given 1/2 dose of ultra-micro powder of Qiweibaishusan; and those in the normal group and model group were given steriled water, all through intragastric administration. After three days the?intestinal microflora were extracted, and their metabolism were determined by Biolog Microbial Identification System. Results showed that the AWCD of all groups tended to stabilize through 72 h of cultivating, with the normal group > the 1/2 dose of ultra-micro powder group > the traditional decoction group > the model group. The microbial diversity index including Shannon index, Shannon evenness, Simpson index and Mclntosh index of the normal group were the largest, significantly different from other groups (P < 0.01). The Shannon index, Simpson index of the 1/2 dose of ultra-micro powder group and the Shannon evenness, Mclntosh index of the traditional decoction group were in the second. The results of clustering analysis and principal components analysis suggested obvious bacteriostasis of antibiotics that influenced some metabolism pathways of intestinal microbial in mice influenced. The metabolism diversity of intestinal microbial in mice gradually regained balance through the regulatory functions of 1/2 dose of ultra-micro powder and traditional decoction of Qiweibaishusan. The regulation role of 1/2 dose of ultra-micro powder was more notable than that of the traditional decoction. The Ultra-micro Qiweibaizhusan is effective in regulating metabolism diversity of intestinal microbial in mice and treating diarrhea with intestinal dysbacteriosis.

参考文献/References:

1 郭维军. 七味白术散加减治疗肠易激综合征脾胃虚弱证的临床观察[J]. 湖南中医药大学学报, 2010, 30 (9): 175-177 [Guo WJ. Effect of modified Qiweibaizhu powder on irritable bowel syndrome with weakened spleen and stomach [J]. J Trad Chin Med Univ Hunan, 2010, 30 (9): 175-177]
2 蔡光先, 杨永华. 中药饮片改革的新探索——单味中药超微饮片[J]. 湖南中医杂志, 2001, 17 (6): 50 [Cai GX, Yang YH. The explore of reform for the traditional Chinese medicine-single ultra-micro powder of traditional Chinese medicine [J]. Hunan J Trad Chin Med, 2001, 17 (6): 50]
3 邓红洁, 吴海, 彭晓珊, 蔡光先, 张熙, 谭周进, 欧阳锋. 超微七味白术散对菌群失调小鼠的疗效研究[J]. 现代生物医学进展, 2011, 11 (19): 3632-3635 [Deng HJ, Wu H, Peng XS, Cai GX, Zhang X, Tan ZJ, Ouyang F. Effects of ultra-micro powder of Qiweibaizhu on dysbacteriosis in mice with diarrhea [J]. Progr Mod Biomed, 2011, 11 (19): 3632-3635]
4 Backhed F. Addressing the gut microbiome and implications for obesity [J]. Intern Dairy J, 2010, 20: 259-261
5 Paliy O, Kenche H, Abernathy F, Michail S. High-throughput quantitative analysis of the human intestinal microbiota with a phylogenetic microarray [J]. Appl Environ Microbiol, 2009, 75 (11): 3572-3579
6 夏小燕, 居文政. 肠道菌群对中药皂苷类成分的代谢研究进展[J]. 中国中医药杂志, 2008, 15 (2): 96-98 [Xia XY, Ju WZ. The research for the effiect of intestinal flora on the metabolism of saponins in intestinal flora metabolism of Chinese medicine saponins in traditional Chinese medicine [J]. China J Chin Mat Med, 2008, 15 (2): 96- 98]
7 Bae EA, Shin JE, Kim DH. Metabolism of ginsenoside Re by human intestinal microflora and its estrogenic effect [J]. Biol Pharm Bull, 2005, 28 (10): 1903-1908
8 Li M, Wang B, Zhang M, Rantalainen M, Wang S, Zhou H, Zhang Y, Shen J, Pang X, Zhang M, Hua W, Yu C, Lu H, Zuo J, Su M, Qiu Y, Jia W, Xiao C, Smith ML, Yang S, Holmes E, Tang H, Zhao G, Nicholson JK, Li L, Zhao L. Symbiotic gut microbes modulate human metabolic phenotypes [J]. Proc Natl Acad Sci USA, 2008, 105 (6): 2117-2122
9 Nicholson JK, Wilson ID. Understanding globalsystems biology:metabonomics and the continuum of metabolism [J]. Nat Rev Drug Discovery, 2003, 2 (8): 668-6761
10 Fernie AR, Trethewey RN, Krotzky AJ, Willmitzer L. Metabolite profiling: from diagnostics to systems biology [J]. Nat Rev Mol Cell Biol, 2004, 5 (9): 763-769
11 程池, 杨梅, 李金霞, 姚粟, 胡海蓉. Biolog微生物自动分析系统-细菌鉴定操作规程的研究[J]. 食品与发酵工业, 2006, 32 (5): 50-54 [Cheng C, Y M, Li JX, Yao S, Hu HR. Biolog Microbial Identification System-Study on the operating regulation of bacteria identification [J]. Food Ferment Ind, 2006, 32 (5): 50-54]
12 胡桂林, 王德良, 张雪峰, 段开红. 用Biolog微生物自动分析系统鉴定大曲中地衣芽孢杆菌的研究[J]. 酿酒, 2007, 34 (6): 32-33 [Hu GL, Wang DL, Zhang XF, Duan KL. Research of Bacillus licheniformis can be identification by Biolog microbial identification system in Daqu [J]. Liquor Making, 2007, 34 (6): 32-33]
13 柏中中, 许婷婷, 何小丹, 何冰芳. 利用Biolog系统进行乳酸生产菌代谢能力的快速分析[J]. 食品与生物技术学报, 2009, 28 (3): 347-351 [Bai ZZ, Xu TT, He XD, He BF. Fast analysis of metabolic abilities of lactate-producing bacteria by Biolog system [J]. J Food Sci Biotechnol, 2009, 28 (3): 347-351]
14 曾奥, 张华玲, 谭周进, 蔡莹, 蔡光先, 周赛男. 小鼠菌群失调腹泻模型的建立及超微七味白术散的疗效[J]. 微生物学通报, 2012, 39 (9): 1341-1348 [Zeng A, Zhang HL, Tan ZJ, Cai Y, Cai GX, Zhou SN. The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro Qiweibaizhusan [J]. Inst Microbiol, 2012, 39 (9): 1341-1348]
15 安韶山, 李国辉, 陈利顶. 宁南山区典型植物根际与非根际土壤微生物功能多样性[J]. 生态学报, 2011, 31 (18): 5225-5233 [An SS, Li GH, Chen LD. Soil microbial functional diversity between rhizosphere and non-rhizosphere of typical plants in he hilly area of southern Nixia [J]. Acta Ecol Sin, 2011, 31 (18): 5225-5234]
16 Classen AT, Boyle SI, Haskins KE, Overby ST, Hart S. Community-level physiological profiles of bacteria and fungi: plate type and incubation temperature influences on contrasting soils [J]. FEMS Microbiol Ecol, 2003, 44 (3): 319-328
17 刘峰, 张兰英, 刘鹏, 刘莹莹, 高松. 采用Biolog法分析制药废水处理工艺中微生物多样性[J]. 中国给水排水, 2007, 23 (21): 28-32 [Liu F, Zhang LY, Liu P, Liu YY, Gao S. Biolog method for analysis of microbial diversity during treatment of pharmaceutical wastewater [J]. China Water & Wastewater, 2007, 23 (21): 28-32]
18 Grove JA, Kautola H, Javadpour S, Young MM, Anderson WA. Assessment of changes in the microorganism community in a biofilter [J]. Biochem Eng J, 2004, 18 (2): 111-114
19 Magurran A E. Ecological Diversity and its Measurement [M]. New Jersey: Princeton University Press, 1988. 43-47
20 Benizri E, Amiaud B. Relationship between plants and soil microbial communities in fertilized grasslands [J]. Soil BiolBiochem, 2005, 37 (11): 2055-2064
21 谭周进, 张华玲, 余望贻, 周赛男, 曾奥, 蔡莹, 蔡光先. 菌群失调小鼠腹泻造模及超微中药干预过程中肠道微生物的变化[J]. 应用与环境生物学报, 2013, 19 (3): 449-453 [Tan ZJ, Zhang HL, Yu WY, Zhou SN, Zeng A, Cai Y, Cai GX. The change of intestinal microbes in mice with dysbacteriosis dodeled and treated by ultra-micro powder of Qiweibaizhusan [J]. Chin J Appl Environ Biol, 2013, 19 (3): 449-453]
22 Vitali B, Ndagijimana M, Cruciani F, Carnevali P, Candela M, Guerzoni ME, Brigidi P. Impact of a synbiotic food on the gut microbial ecology and metabolic profiles [J]. BMC Microbiol, 2010, 10 (4): 1470-1483
23 杨莺莺, 李卓佳, 梁晓华, 梁润捷, 洪敏娜. 芽胞杆菌对鱼池微生物群落代谢功能的影响[J]. 微生物学杂志, 2009, 29 (3): 11-17 [Yang YY, Li ZJ, Liang XH, Liang RJ, Hong MN. Influences of Bacillus on metabolic functions of microbial communities in fishponds [J]. J Microbiol, 2009, 29 (3): 11-17]
24 Fval F, Makivuokko H, Siljander-Rasi H, Putaala H, Tiihonen K, Stowell J, Gibson G,Rautonen N. Effect of polydextrose on intestinal microbes and immune functions in pigs [J]. Br J Nutr, 2007, 98: 123-133
25 张利娟, 王志祥. 中草药制剂对蛋鸡肠道酶活和肠道微生物的影响[J]. 东北农业大学学报, 2010, 4l (6): 94-97 [Zhang LJ, Wang ZX. Effect of compound herbal medicine on intestinal enzyme activity and intestinal microflora in hens [J]. J Northeast Agric Univ, 2010, 4l (6): 94-97]

相似文献/References:

[1]赵兴兵,吴维佳,肖嫩群,等.超微铁皮石斛对脾虚便秘小鼠肠道细菌分子多样性的影响[J].应用与环境生物学报,2015,21(01):170.[doi:10.3724/SP.J.1145.2013.12035]
 ZHAO Xingbing,WU Weijia,XIAO Nenqun,et al.Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):170.[doi:10.3724/SP.J.1145.2013.12035]
[2]郭抗萧,肖新云,刘又嘉,等.七味白术散对菌群失调腹泻小鼠肠道乳酸杆菌多样性的影响[J].应用与环境生物学报,2015,21(06):1071.[doi:10.3724/SP.J.1145.2015.04030]
 GUO Kangxiao,XIAO Xinyun,LIU Youjia,et al.Effect of Qiweibaizhusan on the intestinal lactobacilli diversity in dysbacteria diarrheal mice[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):1071.[doi:10.3724/SP.J.1145.2015.04030]
[3]谭周进,张华玲,周赛男,等.菌群失调小鼠腹泻造模及超微中药干预过程中肠道微生物的变化[J].应用与环境生物学报,2013,19(03):449.[doi:10.3724/SP.J.1145.2013.00449]
 TAN Zhoujin,ZHANG Hualing,ZHOU Sainan,et al.Change of Intestinal Microbes in Dysbacteriosis-modeled Mice Treated with Ultra-micro Powder of Qiweibaizhusan[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):449.[doi:10.3724/SP.J.1145.2013.00449]

备注/Memo

备注/Memo:
国家自然科学基金项目(81173214)资助
更新日期/Last Update: 2014-03-06