|本期目录/Table of Contents|

[1]马海林,邢尚军,刘方春,等.冬枣(Ziziphus jujube Mill.)根际3株促生细菌的筛选与鉴定[J].应用与环境生物学报,2013,19(04):650-654.[doi:10.3724/SP.J.1145.2013.00650]
 MA Hailin,XING Shangjun,LIU Fangchun,et al.Screening and Identification of Three Plant Growth-promoting Rhizobacteria from Winter Jujube (Ziziphus jujube Mill.) Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):650-654.[doi:10.3724/SP.J.1145.2013.00650]
点击复制

冬枣(Ziziphus jujube Mill.)根际3株促生细菌的筛选与鉴定()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年04期
页码:
650-654
栏目:
研究论文
出版日期:
2013-08-25

文章信息/Info

Title:
Screening and Identification of Three Plant Growth-promoting Rhizobacteria from Winter Jujube (Ziziphus jujube Mill.) Rhizosphere
作者:
马海林邢尚军刘方春陈波丁延芹姚良同杜秉海
(1山东省林业科学研究院,山东省森林植被生态修复工程技术研究中心 济南 250014)
(2山东农业大学生命科学学院 泰安 271018)
Author(s):
MA Hailin XING Shangjun LIU Fangchun CHEN Bo DING Yanqin YAO Liangtong DU Binghai
(1Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forestry, Jinan 250014, China)
(2College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China)
关键词:
冬枣根际促生细菌菌种筛选菌种鉴定激素
Keywords:
winter jujube (Ziziphus jujube Mill.) rhizosphere growth-promoting rhizobacteria strain screening strain identification hormone
分类号:
S144 : S665.106
DOI:
10.3724/SP.J.1145.2013.00650
文献标志码:
A
摘要:
为给冬枣(Ziziphus jujube Mill.)生物肥料的研制和生产提供优良菌株资源,利用梯度稀释法和三区划线法从冬枣根际土壤中分离、纯化出832株细菌分离物,进一步通过小麦叶片保绿法和萝卜子叶增重法筛选出效果较好的3株,分别记为DZ1、DZ2和DZ3. 这3株细菌分离物均不产异戊烯基腺嘌呤,其中DZ1和DZ2分泌反式玉米素(分别为368.73和598.46 ng mL-1)、激动素(273.56和193.16 ng mL-1)和吲哚乙酸[9.24和13.85 μg mL–1 (OD600 nm)–1],而DZ3分泌激动素(175.4 ng mL-1)和反式玉米素(314.34 ng mL-1),但不产吲哚乙酸. 通过形态学观察、生理生化测定、16S rRNA基因测序及系统发育树分析,初步确定DZ1和DZ2为枯草芽孢杆菌(Bacillus subtilis),DZ3为芽孢杆菌属(Bacillus sp.). 最后通过盆栽试验对这3株细菌分离物的促生效果进行比较,结果发现,DZ1、DZ2和DZ3,尤其是DZ1,对冬枣细根的生长及地上部干物质积累有积极作用,可作为研制冬枣生物肥料的优良菌株资源. 图2 表3 参21
Abstract:
The aim of the present study was to provide the excellent strain resources for the production of winter jujube (Ziziphus jujube Mill.) bio-fertilizer. Plant growth-promoting rhizobacteria (PGPR) are important catalysts that regulate the functional properties of agricultural systems. However, there is little information on the isolation and screening from winter jujube rhizospheric soil. Therefore, bacterial isolates were isolated and screened from winter jujube rhizospheric soil by the methods of remaining green and radish cotyledon bioassay. First, 832 bacterial isolates were separated and purified from the rhizosphere soil using serial dilution and three-region streaking methods. Then 3 PGPR, with broad application prospects in the bio-fertilizer production, were screened and named DZ1, DZ2 and DZ3, respectively. The plant hormones produced by the bacterial isolates were quantitatively detected. At last, the three PGPR were identified based on the results of morphologic characteristics, physiological biochemical properties and phylogenetic analysis of 16S rRNA genes sequence. The results showed that none of the three bacterial isolates produced isopentennyladenine. DZ1 and DZ2 produced trans-zeatin (368.73 and 598.46 ng mL-1, respectively), kinetin (273.56 and 193.16 ng mL-1, respectively), and indoleacetic acid (9.24 and 13.85 μg mL–1 (OD600 nm)–1, respectively). DZ3 produced trans-zeatin (175.4 ng mL-1), and kinetin (314.34 ng mL-1), but no indoleacetic acid. According to the results of morphologic characteristics, physiological biochemical properties and phylogenetic analysis of 16S rRNA genes, DZ1 and DZ2 were identified as Bacillus subtilis, and DZ3 identified as Bacillus sp. The pot experiment results showed that the three PGPR, especially DZ1, can significantly improve the fine root and aerial part dry weight, beneficial to winter jujube growth. In conclusion, DZ1, DZ2, and DZ3 exert beneficial effects on winter jujube growth, exhibiting broad application prospects in the winter jujube bio-fertilizer production. Fig 3, Tab 2, Ref 21

参考文献/References:

1 Verma JP, Yadav J, Tiwari KN, Lavakush SV. Impact of plant growth promoting rhizobacteria on crop production [J]. Int J Agric Res, 2010, 5: 954-983
2 康贻军, 程洁, 梅丽娟, 殷士学, 植物根际促生菌的筛选及鉴定[J]. 微生物学报, 2010, 50 (7): 853-861 [Kang YJ, Cheng J, Mei LJ, Yin SX. Screening and identification of plant growth-promoting rhizobacteria [J]. Acta Microbiol Sin, 2010, 50 (7): 853-861]
3 Abbasi MK, Sharif S, Kazmi M. Sultan T, Aslam M. Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield and nutrient uptake of plants [J]. Plant Biosyst, 2011, 145: 159-168
4 Egamberdiyeva D. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils [J]. Appl Soil Ecol, 2007, 36: 184-189
5 Karakurt H, Kotan R. Effects of plant growth promoting rhizobacteria on fruit set, pomological and chemical characteristics, color values, and vegetative growth of sour cherry (Prunus cerasus cv. Kütahya) [J]. Turk J Biol, 2011, 35: 283-291
6 刘方春, 邢尚军, 马海林, 杜振宇, 马丙尧, 段春华, 陈波. 生物肥对冬枣生物学特性及产量和品质的影响[J]. 水土保持学报, 2010, 24 (6): 222-226 [Liu FC, Xing SJ, Ma HL, Du ZY, Ma BY, Duan CH, Chen B. Effects of biological organic fertilizer on biological characteristics, yield and quality of Zizyphus jujube Mill. Dongzao trees [J]. J Soil Water Conserv, 2010, 24 (6): 222-226]
7 魏绍冲, 姜远茂. 冬枣果实中1个ETR2类乙烯受体基因的克隆及其表达[J]. 林业科学, 2012, 48 (4): 138-142 [Wei SC, Jiang YM. Cloning and expression of a cDNA encoding ETR2-Type ethylene reception in ‘Dongzao’ jujube [J]. Sci Silv Sin, 2012, 48 (4): 138-142]
8 刘方春, 邢尚军, 马海林, 杜振宇, 马丙尧, 陈波. 生物肥对冬枣根际土壤微环境特征的影响[J]. 北京林业大学学报, 2012, 34 (5): 102-106 [Liu FC, Xing SJ, Ma HL, Du ZY, Ma BY, Chen B. Effect of bio-fertilizers on micro-environment characteristics in Zizyphus jujube Mill. rhizosphere soil [J]. J Beijing For Univ, 2012, 34 (5): 102-106]
9 Adesemoye AO, Torbert HA, Kloepper JW. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microb Ecol, 2009, 58: 921-929
10 Kumara S, Pandeyb P, Maheshwari DK. Maheshwari DK. Reduction in dose of chemical fertilizers and growth enhancement of sesame (Sesamum indicum L.) with application of rhizospheric competent Pseudomonas aeruginosa LES4. Eur J Soil Biol, 2009, 45: 334-340
11 张慧, 杨兴明, 冉炜, 徐阳春, 沈其荣. 土传棉花黄萎病拮抗菌的筛选及其生物效应[J]. 土壤学报, 2008, 45 (6): 1095-1101 [Zhang H, Yang XM, Ran W, Xu YC, Shen QR. Screening of bacteria antagonistic against soil-borne cotton verticillium wilt and their biological effects on the soil-cotton system [J]. Acta Pedol Sin, 2008, 45 (6): 1095-1101]
12 Bari R, Jones JDG . Role of plant hormones in plant defence responses [J]. Plant Mol Biol, 2009, 69: 473-488
13 Garrity GM, Bell JA, Lilburn TG. Taxonomic Outline of the Prokaryotes Bergey’s Manual of Systematic Bacteriology [M]. 2nd ed. New York: Heidelberg Springer-verlag, 2004
14 刘琳, 孙磊, 张瑞英, 姚娜, 李潞滨. 春兰根中可分泌吲哚乙酸的内生细菌多样性[J]. 生物多样性, 2010, 18 (2): 195-200 [Liu L, Sun L, Zhang RY, Yao N, Li LB. Diversity of IAA-producing endophytic bacteria isolated from the roots of Cymbidium goeringii [J]. Biodiv Sci, 2010, 18 (2): 195-200]
15 Arkhipova TN, Prinsen E, Veselov SU, Martinenko EV, Melentiev AI, Kudoyarova GR. Cytokinin producing bacteria enhance plant growth in drying soil [J]. Plant Soil, 2007, 292: 305-315
16 Hussain A, Hasnain S. Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria [J]. World J Microbiol Biotechnol, 2011, 27: 2645-2654
17 Hussain A, Hasnain S. Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria [J]. World J Microbiol Biotechnol, 2011, 27: 2645-2654
18 Sudhakar P, Chattopadhyay GN, Gangwar SK, Ghosh JK. Effect of foliar application of Azotobacter, Azospirillum and Beijerinckia on leaf yield and quality of mulberry (Morus alba) [J]. J Agric Sci, 2000, 134: 227-234
19 王丹, 李旺, 巩春甫, 杨四佳, 张强, 唐恺迪, 戚南昌, 银丽娟, 张杰, 杨志荣. 不对称还原丙酮酸乙酯菌株的筛选及鉴定[J]. 应用与环境生物学报, 2012, 18 (3): 460-464 [Wang D, Li W, Gong CF, Yang SJ, Zhang Q, Tang KD, Qi NC, Yin LJ, Zhang J, Yang ZR. Screening and identification of a strain for asymmetric reduction of ethyl pyruvate [J]. Chin J Appl Environ Biol, 2012, 18 (3): 460-464]
20 Karakurt H, Kotan R. Effects of plant growth promoting rhizobacteria on fruit set, pomological and chemical characteristics, color values, and vegetative growth of sour cherry (Prunus cerasus cv. Kütahya) [J]. Turk J Biol, 2011, 35: 283-291
21 Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities [J]. Microbiol Res, 2008, 163 (2): 173-181

相似文献/References:

[1]盛江梅,吴小芹,侯亮亮,等.一株黑松-美味牛肝菌菌根辅助细菌的筛选及鉴定[J].应用与环境生物学报,2010,16(05):701.[doi:10.3724/SP.J.1145.2010.00701]
 SHENG Jiangmei,WU Xiaoqin,HOU Liangliang,et al.Isolation and Identification of a MHB Strain from the Rhizosphere Soil of Pinus thunbergi Inoculated with Boletus edulis[J].Chinese Journal of Applied & Environmental Biology,2010,16(04):701.[doi:10.3724/SP.J.1145.2010.00701]
[2]刘方春,邢尚军,马海林,等.PGPR生物肥对甜樱桃(Cerasus pseudocerasus)根际土壤生物学特征的影响[J].应用与环境生物学报,2012,18(05):722.[doi:10.3724/SP.J.1145.2012.00722]
 LIU Fangchun,XING Shangjun,MA Hailin,et al.Effect of PGPR Fertilizer on Biological Characteristics in Cerasus pseudocerasus Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):722.[doi:10.3724/SP.J.1145.2012.00722]
[3]马海林,刘方春,马丙尧,等.保水剂对侧柏容器苗根际土壤微生物种群结构及干旱适应能力的影响[J].应用与环境生物学报,2016,22(01):43.[doi:10.3724/SP.J.1145.2015.04009]
 MA Hailin**,LIU Fangchun,MA Bingyao,et al.Effects of super-absorbent polymer on the microbial community structure in rhizosphere soil and drought resistance of platycladus orientalis container seedlings[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):43.[doi:10.3724/SP.J.1145.2015.04009]
[4]李善家,王军强,施志国,等.不同基肥处理对玉米土壤酶活性和球囊霉素相关土壤蛋白的影响[J].应用与环境生物学报,2017,23(2):357.[doi:10.3724/SP.J.1145.2016.04045]
 LI Shanjia,WANG Junqiang,et al.Effect of different base fertilizer treatments on maize soil enzyme activity and glomalin-related protein[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):357.[doi:10.3724/SP.J.1145.2016.04045]
[5]杨永,张学军,李寐华,等.微生物肥料对设施长期连作哈密瓜根际土壤真菌群落结构的影响[J].应用与环境生物学报,2018,24(01):68.[doi:10.19675/j.cnki.1006-687x.2017.03014]
 YANG Yong,ZHANG Xuejun,LI Meihua,et al.Effects of microbiological fertilizer on rhizosphere soil fungus communities under long-term continuous cropping of protected Hami melon[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):68.[doi:10.19675/j.cnki.1006-687x.2017.03014]
[6]李静,张金羽,张琪,等.玉米根际无机磷溶解菌的筛选与促生特性[J].应用与环境生物学报,2019,25(02):378.[doi:10.19675/j.cnki.1006-687x.2018.06005]
 LI Jing,ZHANG Jinyu,ZHANG Qi,et al.Screening and analysis of plant growth-promoting properties in inorganic phosphate-solubilizing bacteria from maize rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):378.[doi:10.19675/j.cnki.1006-687x.2018.06005]
[7]樊利华,周星梅,吴淑兰,等.干旱胁迫对植物根际环境影响的研究进展[J].应用与环境生物学报,2019,25(05):1244.[doi:10.19675/j.cnki.1006-687x.2018.12037]
 FAN Lihua,ZHOU Xingmei,WU Shulan,et al.Research advances on the effects of drought stress in plant rhizosphere environments[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1244.[doi:10.19675/j.cnki.1006-687x.2018.12037]
[8]周思婕,王平,张敏,等.酸胁迫对马尾松幼苗生长及根际铝形态的影响[J].应用与环境生物学报,2019,25(06):1292.[doi:10.19675/j.cnki.1006-687x.2019.01024]
 ZHOU Sijie,WANG Ping**,ZHANG Min,et al.Effects of acid stress on growth and aluminum speciation in the rhizosphere of Masson pine (Pinus massoniana L.) seedlings[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1292.[doi:10.19675/j.cnki.1006-687x.2019.01024]
[9]燕红梅 张欣钰 檀文君 陈卫民**.5种不同植物根际真菌群落结构与多样性*[J].应用与环境生物学报,2020,26(01):1.[doi:10.19675/j.cnki.1006-687x.2019.05010]
 YAN Hong mei,ZHANG Xin yu,et al.Biodiversity and composition of rhizosphere fungi community associated with five plant species*[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):1.[doi:10.19675/j.cnki.1006-687x.2019.05010]

备注/Memo

备注/Memo:
山东省科学技术发展计划项目(2007GG2009007)和山东省农业重大应用技术创新项目资助 Supported by the Science and Technology Development Project of Shandong, China (No. 2007GG2009007) and the Major Special Project for Agricultural Application Technology of Shandong
更新日期/Last Update: 2013-08-22