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[1]龚志婷,赖泳红,周艳梅,等.云南保山温泉产纤维素酶高温菌多样性研究[J].应用与环境生物学报,2013,19(04):699-703.[doi:10.3724/SP.J.1145.2013.00699]
 GONG Zhiting,LAI Yonghong,ZHOU Yanmei,et al.Diversity of Cellulose-producing Thermophilic Bacteria in the Baoshan Hot Spring[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):699-703.[doi:10.3724/SP.J.1145.2013.00699]
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云南保山温泉产纤维素酶高温菌多样性研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年04期
页码:
699-703
栏目:
研究论文
出版日期:
2013-08-25

文章信息/Info

Title:
Diversity of Cellulose-producing Thermophilic Bacteria in the Baoshan Hot Spring
作者:
龚志婷赖泳红周艳梅李鹏郑伟光王天强崔晓龙王永霞肖炜
(1云南大学生命科学学院 昆明 650091)
(2云南大学微生物研究所西南微生物多样性教育部重点实验室 昆明 650091)
Author(s):
GONG Zhiting LAI Yonghong ZHOU Yanmei LI Peng ZHENG Weiguang WANG Tianqiang CUI Xiaolong WANG Yongxia XIAO Wei
(1College of Life Sciences, Yunnan University, Kunming 650091, China)
(2Key Laboratory of Microbial Diversity in Southwest China, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China)
关键词:
高温菌微生物多样性纤维素酶淀粉酶蛋白酶温泉
Keywords:
thermophilic bacteria microbial diversity cellulase amylase protease hot spring
分类号:
Q938 (274)
DOI:
10.3724/SP.J.1145.2013.00699
文献标志码:
A
摘要:
为了探寻温泉中的未知菌种,寻找能用于工业生产的产酶菌株,分别对云南省保山市腾冲县和昌宁县温泉中产纤维素酶高温菌进行筛选分离鉴定,并研究其多样性. 采用不同的温度和pH培养条件,从温泉中筛选出具有纤维素分解能力的菌株200株. 通过对菌株的16S rRNA基因序列测序鉴定分析,确定其分属于8个属30个种. 在55 ℃培养条件下分离获得菌株122株,分别属于8个属26个种. 在65 ℃条件下分离获得菌株78株,分别属于5个属15个种. 菌株纯化转接4代后检测产酶活性,95%以上产纤维素酶的菌株同时具有淀粉酶的分解能力,50%左右的产纤维素酶菌株同时产蛋白酶. 研究表明在腾冲温泉和昌宁温泉中,存在着较多产纤维素酶、淀粉酶、蛋白酶的菌株. 图3 表3 参21
Abstract:
To obtain amylase-producing, cellulase-producing and protease-producing thermophilic bacteria, two kinds of separation medium were used on different temperature and pH by spread-pate technique. 16S rRNA genes of these bacteria were obtained by PCR amplification with universal primers. The obtained PCR products were sequenced and blasted in GenBank. Based on 16S rRNA gene sequences, 3 phylogenetic trees were constructed to analyze the taxonomy of thermophilic bacteria. All together 200 cellulose-producing strains were isolated from the two main hot springs in Baoshan, Yunnan Province of China. All the 200 strains belonged to 30 species. Among them 122 stains were isolated from culture conditions of 55 ℃, 78 stains from culture conditions of 65 ℃, belonging to 15 species. The result showed that more than 95% cellulase-producing strains produce amylase, and about 50% of cellulase-producing strains produce protease. In hot spring, cellulase-producing, amylase-producing, and protease-producing strains exist extensively. Fig 3, Tab 3, Ref 21

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备注/Memo

备注/Memo:
云南省科技条件平台建设计划项目(2009DA002)、教育部国家级大学生创新项目(X3108052-91067319)、云南大学生命科学学院科学研究与人才培养开放基金项目(2012S203)、云南省烟草公司科技项目(2011YN26)和云南省教育厅基金项目(2011Y105)资助
更新日期/Last Update: 2013-08-22