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[1]赵永云,杜凤,董娟,等.孔雀石绿和结晶紫在荧光检测中的比较[J].应用与环境生物学报,2013,19(02):365-369.[doi:10.3724/SP.J.1145.2013.00365]
 ZHAO Yongyun,DU Feng,DONG Juan,et al.Comparison of Two Triphenylmethane Dye (Malachite Green and Crystal Violet) in Fluorescence Detection[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):365-369.[doi:10.3724/SP.J.1145.2013.00365]
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孔雀石绿和结晶紫在荧光检测中的比较()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年02期
页码:
365-369
栏目:
技术与方法
出版日期:
2013-04-25

文章信息/Info

Title:
Comparison of Two Triphenylmethane Dye (Malachite Green and Crystal Violet) in Fluorescence Detection
作者:
赵永云杜凤董娟陈浩东周丽陈蓉唐卓
(中国科学院成都生物研究所 成都 610041)
Author(s):
ZHAO Yongyun DU Feng DONG Juan CHEN Haodong Zhou Li CHEN Rong TANG Zhuo
(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
孔雀石绿结晶紫G-四聚体链置换等温扩增实时荧光检测
Keywords:
malachite green crystal violet G-quadruplexe strand displacement amplification real-time fluorescence
分类号:
Q503 : Q78
DOI:
10.3724/SP.J.1145.2013.00365
文献标志码:
A
摘要:
为优选出三苯甲烷染料中的一种染料与G-四聚体结合开发为荧光定量报告体系,对孔雀石绿(Malachite green,MG)和结晶紫(Crystal violet ,CV)与G-四聚体结合后的灵敏度和重现性进行了比较研究. 首先,比较了4种不同DNA(hum21,short-hum21,double-strands,single-strand)分别与孔雀石绿和结晶紫结合后的荧光光谱,结果显示,两种染料均可区分以上4种DNA,但孔雀石绿的灵敏度更高. 然后,将孔雀石绿和结晶紫应用到实时荧光检测中,进一步比较验证两种染料灵敏度,并考察两种染料的重现性,结果显示,孔雀石绿的重现性和灵敏度均优于结晶紫. 由此,建立了孔雀石绿与G-四聚体结合的荧光定量报告体系. 图4 表1 参21
Abstract:
In order to select a triphenylmethane (TPM) dye to bind to G-quadruplex for developing a fluorescent quantitative reporting system, we compared the sensitivity and reproducibility of two TPM dyes, malachite green and crystal violet, binding to G-quadruplex. First, ?uorescence spectra of the two TPM dyes were compared in the presence of G-quadruplexes (hum21, short-hum21), single-strand and double-strands DNA. The results showed that both TPM dyes could discriminate the four DNAs with the sensitivity of malachite green higher than that of crystal violet. Then malachite green and crystal violet were applied to real-time fluorescence detection, which showed that malachite green had higher sensitivity and reproducibility than crystal violet. As a result, our proposed fluorescent quantitative reporting system is composed by malachite green and the G-quadruplex. Fig 4, Tab 1, Ref 21

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备注/Memo

备注/Memo:
中国科学院知识创新工程项目(KSCX2-EW-J-22)资助
更新日期/Last Update: 2013-05-03