|本期目录/Table of Contents|

[1]王芳,刘晓飞,刘晓风,等.产氢菌对沼气发酵的生物强化作用[J].应用与环境生物学报,2013,19(02):351-355.[doi:10.3724/SP.J.1145.2013.00351]
　WANG Fang,LIU Xiaofei,LIU Xiaofeng,et al.Biotechnological Intensification of Biogas Fermentation by Hydrogen Producing Bacteria[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):351-355.[doi:10.3724/SP.J.1145.2013.00351]
点击复制

产氢菌对沼气发酵的生物强化作用()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年02期
页码:
351-355
栏目:
研究论文
出版日期:
2013-04-25

文章信息/Info

Title:
Biotechnological Intensification of Biogas Fermentation by Hydrogen Producing Bacteria
作者:
王芳; 刘晓飞; 刘晓风; 袁月祥; 闫志英; 贺蓉娜; 廖银章
（1中国科学院成都生物研究所 成都 610041）
（2辽宁省凌源市农村能源、农业区划办公室 凌源 122500）
Author(s):
WANG Fang;  LIU Xiaofei;  LIU Xiaofeng;  YUAN Yuexiang;  YAN Zhiying;  HE Rongna;  LIAO Yinzhang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Rural Energy and Agricultural Zone Planning Office of Lingyuan City, Lingyuan 122500, Liaoning, China)
关键词:
沼气发酵; 产氢菌; 产甲烷菌; 甲烷产量; 挥发性有机酸
Keywords:
biogas fermentation;  hydrogen producing bacteria;  methane producing bacteria;  methane yield;  volatile fatty acid
分类号:
S216.4 : Q939.9
DOI:
10.3724/SP.J.1145.2013.00351
文献标志码:
A
摘要:
在以猪粪为底物的沼气发酵系统中，投加产氢菌Clostridium sp. WY-1与Clostridium sp. WW-5混合后离心的菌体，加入量以两株菌的混合菌液计，分别为发酵液总体积的2%、4%、8%、16%，30 ℃下发酵30 d，研究产氢菌投加对产甲烷系统的生物强化效应. 结果表明，2%、4%、8%、16%四种不同投加量均能提高甲烷总产量，累积甲烷产量比对照分别增加6%、11%、4%、3%，其中投加量4%增加最高；而对甲烷浓度无明显提高. 在发酵前3 d，各组中均能检测到少量的氢气，投加产氢菌各组中d 2的氢气含量均比对照高，d 3对照组、2%和4%组检测不出氢气，但8%、16%组仍有极少量氢气存在. 总挥发性有机酸在发酵过程中总体趋势是不断下降，发酵前20 d投加产氢菌组的总挥发性有机酸含量均比对照组高. 投加产氢菌各组中产氢菌和产甲烷菌的数量与对照组相比均有所增加，而投加量4%组中产氢菌和产甲烷菌的数量比投加2%、8%和16%中的产氢菌和产甲烷菌的数量高. 图7 表3 参15
Abstract:
In order to study the biotechnological intensification of hydrogen producing bacteria (HPB), two HPBs isolated by our laboratory, Clostridium sp. WY-1and Clostridium sp. WW-5, were introduced into the methane fermentation system with pig manure as substrate. Besides the control without HPB addition, four levels of the mixture of the two HPBs were applied in this trial, i.e. 2%, 4%, 8% and 16% of HPB mixture in the fermentation liquid. The mixtures of the liquid were fermented for 30 days at 30 ℃. Results showed that the accumulative methane production of 2%, 4%, 8% and 16% of the HPB treatments were 6%, 11%, 4% and 3% respectively higher than that of the control, but methane concentration was not enhanced significantly. It suggested that 4% of HPB was the optimal proportion. H2 was detected during the first three days in all five groups. On the second day the H2 content of 2%, 4%, 8% and 16% of HPB treatment was higher than that of the control; on the third day H2 was detected in the 8% and 16% of HPB treatments, but not in the control, 2% and 4% groups. The amount of volatile fatty acid (VFA) was decreasing during the fermentation period in all five groups, with the concentration of VFA of all HPB treatments higher than that of the control in the first 20 days. The amounts of HPB and MPB in 2%, 4%, 8% and 16% HPB treatments were both higher than those of the control; and the highest amount of the two types of bacteria was detected in the 4% HPB treatment. Fig 7, Tab 3, Ref 15

参考文献/References:

1 Geeta GS, Jagadeesh KS, Reddy TKR. Nickel as an accelerator of biogas production in water hyacinth (Eichornia crassipes solms) [J]. Biomass, 1990, 21 (2): 157-161
2 Pfeffer JT. Temperature effects on anaerobic fermentation of domestic refuse [J]. Biotechnol Bioeng, 1974, 16 (6): 771-787
3 Chen TH, Hashimoto AG. Effects of pH and substrate: inoculum ratio on batch methane fermentation [J]. Bioresour Technol, 56 (2-3): 179-186
4 Borja R, Banks CJ, Wang Z. Effect of organic loading rate on anaerobic treatment of slaughterhouse wastewater in a fluidised-bed reactor [J]. Bioresour Technol, 1995, 52 (2): 157-162
5 Ferry JG. Methnogenesis - Ecology, Physiology, Biochemistry and Genetics [M]. New York: Chapman & Hall, 1993. 128-206
6 Bryant MP, Wolin EA, Wolin MJ, Wolfe RS. Methanobacillus omelianskii, a symbiotic association of two species of bacteria [J]. Arch Microbiol, 1967, 59 (1): 20-31
7 Tirumale S, Nand K. Influence of anaerobic cellulolytic bacterial consortia in the anaerobic digesters on biogas production [J]. Biogas Forum Ⅲ, 1994, 58: 12-15
8 Attar Y, Mhetre ST, Shawale MD. Biogas production enhancement by cellulytic strains of Actinomycetes [J]. Biogas Forum Ⅰ, 1998, 72: 11-15
9 Weiß S, Tauber M, Somitsch W, Meincke R, Müller H, Berg G, Guebitz GM. Enhancement of biogas production by addition of hemicellulolytic bacteria immobilised on activated zeolite [J]. Water Res, 2010, 44 (6): 1970-1980
10 Bagi Z, Ács N, Bálint B, Horváth L, Dobó K, Perei K, Rákhely G, Kovács K. Biotechnological intensification of biogas production [J]. Appl Microbiol Biotechnol, 2007, 76 (2): 473-482
11 Norris JR, Ribbons DW. Methods in Microbiology [M]. London: Britain Academic Press, 1969. 117-l32
12 任南琪, 林明, 马汐平, 王爱杰, 李建政. 厌氧高效产氢细菌的筛选及其耐酸性研究[J]. 太阳能学报, 2003 (1): 80-84 [Ren NQ, Lin M, Ma XP, Wang AJ, Li JZ. A strain of bacteria screened for high efficient hydrogen production and its aciduric character [J]. Acta Energy Solaris Sin, 2003 (1): 80-84]
13 Doré J, Pochart P, Bernalier A, Goderel I, Morvan B, Rambaud JC. Enumeration of H2-utilizing methanogenic archaea, acetogenic and sulfate-reducing bacteria from human feces [J]. FEMS Microbiol Ecol, 1995, 17 (4): 279-284
14 Winfrey MR, Nelson DR, Klevickis SC, Zeikus JG. Association of hydrogen metabolism with methanogenesis in Lake Mendota sediments [J]. Appl Environ Microbiol, 1977, 33 (2): 312-318
15 Morris JG. The physiology of obligate anaerobiosis [J]. Adv Microbial Physiol, 1975, 12: 169-246

相似文献/References:

[1]宋丽,刘晓风,刘培旺,等.微波诱变选育耐酸高效厌氧产氢菌[J].应用与环境生物学报,2008,14(03):427.
　SONG Li,et al..Screening of Highly Efficient H2producing Aciduric Anaerobic Strain Using Microwave Mutagenesis[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):427.

备注/Memo

备注/Memo:
环保公益性行业科研专项（201109047）和“十二五”科技支撑计划课题（2011BAD15B03）资助
更新日期/Last Update: 2013-05-03