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[1]黄胜雄,刘永胜.土豆WRKY转录因子家族的生物信息学分析[J].应用与环境生物学报,2013,19(02):205-214.[doi:10.3724/SP.J.1145.2013.00205]
 HUANG Shengxiong,LIU Yongsheng.Genome-wide Analysis of WRKY Transcription Factors in Solanum tuberosum[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):205-214.[doi:10.3724/SP.J.1145.2013.00205]
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土豆WRKY转录因子家族的生物信息学分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年02期
页码:
205-214
栏目:
研究论文
出版日期:
2013-04-25

文章信息/Info

Title:
Genome-wide Analysis of WRKY Transcription Factors in Solanum tuberosum
作者:
黄胜雄刘永胜
(1四川大学生命科学学院/生物资源与生态环境教育部重点实验室,水力学与山区河流开发保护国家重点实验室 成都 610064)
(2合肥工业大学生物与食品工程学院 合肥 230009)
Author(s):
HUANG Shengxiong LIU Yongsheng
(1Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China)
(2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
关键词:
转录因子WRKY土豆进化分析表达模式胁迫防御应答
Keywords:
transcriptional factor WRKY potato phylogenetic analysis expression pattern defense response against stress
分类号:
S532: Q349+.501
DOI:
10.3724/SP.J.1145.2013.00205
文献标志码:
A
摘要:
WRKY转录因子家族属于一类广泛参与植物生长发育调控,以及各种生物和非生物胁迫下植物防御应答反应的转录因子. 通过HMMER 3.0软件,在土豆基因组中进行WRKY转录因子的比对搜索和鉴定. 系统分析鉴定得到81个土豆WRKY转录因子,被划分为3个大组:Ⅰ组、Ⅱ组和Ⅲ组. Ⅱ组进一步划分为5个亚组:Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d和Ⅱ-e. 土豆、拟南芥和水稻的WRKY基因的系统进化树揭示出:土豆和水稻基因组中分别发生了物种特异的WRKY基因扩增事件,其中Ⅱ-e组中发生了土豆基因组中特有基因扩增. 同组中的WRKY成员蛋白序列中的氨基酸保守域构成基本一致. 各个分组成员的氨基酸保守域组成特异;但共有的保守域区段在各个分组中仍然存在. 在受伤、干旱、盐以及病毒侵染胁迫条件下,28个土豆WRKY转录因子基因的表达模式被阐明;其中部分基因的表达水平被显著地诱导增加或者降低,很可能参与了调控土豆逆境胁迫条件下的防御应答反应. 图4 表2 参33
Abstract:
The WRKY transcription factor family is widely involved in regulating plant development and defense response against various biotic and abiotic stresses. The Hidden Markov Model Profile (HMM) of WRKY domain was used to identify WRKY transcription factors in potato genome with HMMER 3.0. All together 81 potato WRKY transcription factors were identified and then classified into three main groups, with 5 subgroups in Group Ⅱ, namely Ⅱ-a, Ⅱ-b, Ⅱ-c, Ⅱ-d, and Ⅱ-e. The constructed phylogenetic tree of WRKY genes among potato, Arabidopsis and rice demonstrated that the distinct gene expansion events occurred in the genome of potato and rice, respectively, while the gene expansion of Group Ⅱ-e was unique in potato genome. The potato WRKY transcription factors showed similar motif compositions within each group, but different ones among different groups, though several conserved motifs were shared by all. Furthermore, 28 potato WRKY genes showed distinct expression patterns in response to stresses of wounding, drought, salt, and virus invasion. The expressions of several potato WRKY genes were significantly up/down-regulated, implying that these members might participate in regulating the defense response against various biotic and abiotic stresses. Fig 4, Tab 2, Ref 33

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备注/Memo

备注/Memo:
国家杰出青年科学基金项目(30825030)、国家自然科学基金项目(31171179)和博士点优先资助项目(20110181130009)资助
更新日期/Last Update: 2013-05-02