|本期目录/Table of Contents|

[1]徐莲,孙纪全,吴晓磊,等.菌株Rhodococcus sp. Chr-9和Exiguobacterium sp. Chr-43的除铬(VI)特性[J].应用与环境生物学报,2012,18(06):971-977.[doi:10.3724/SP.J.1145.2012.00971]
 XU Lian,SUN Jiquan,WU Xiaolei,et al.Characteristics of Removal of Cr (VI) by Rhodococcus sp. Chr-9 and Exiguobacterium sp. Chr-43[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):971-977.[doi:10.3724/SP.J.1145.2012.00971]
点击复制

菌株Rhodococcus sp. Chr-9和Exiguobacterium sp. Chr-43的除铬(VI)特性()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年06期
页码:
971-977
栏目:
研究论文
出版日期:
2012-12-25

文章信息/Info

Title:
Characteristics of Removal of Cr (VI) by Rhodococcus sp. Chr-9 and Exiguobacterium sp. Chr-43
作者:
徐莲 孙纪全 吴晓磊 汤岳琴 陈福明
(1北京大学工学院 北京 100871)
(2深圳清华大学研究院,深圳市工业应用分离技术重点实验室 深圳 518057)
(3清华大学化工系 北京 100084)
(4四川大学建筑与环境学院 成都 610065)
Author(s):
XU Lian SUN Jiquan WU Xiaolei TANG Yueqin CHEN Fuming
(1College of Engineering, Peking University, Beijing 100871, China)
(2Research Institute of Tsinghua University in Shenzhen, Shenzhen Key Laboratory of Separation Technology, Shenzhen 518057, China)
(3Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China)
(4College of Architecture and Environment, Sichuan University, Chengdu, 610065, China)
关键词:
铬(VI)红球菌属微小杆菌生物修复环境因素污水处理
Keywords:
Cr (VI) Rhodococcus sp. Exiguobacterium sp. bioremediation environment factor wastewater treatment
分类号:
X172
DOI:
10.3724/SP.J.1145.2012.00971
文献标志码:
A
摘要:
为了解环境因素对生物除铬(VI)的影响,并为铬(VI)污染环境的生物强化治理提供高效菌株,采用选择培养的方法从制革废水污泥样品中分离到2株革兰氏阳性铬(VI)去除菌——Rhodococcus sp. Chr-9和Exiguobacterium sp. Chr-43. 菌株Chr-9和Chr-43在25~40 ℃内均能够较好生长,并高效去除铬(VI),菌株Chr-9和Chr-43的最适生长pH均为7.0~9.0,菌株在pH 7.0的培养基中去除铬(VI)的效率最高. 加入0.2~0.5 mol/L的NO3-、SO42-和Cl-能够促进Chr-9和Chr-43的生长以及去除铬(VI)的效率. 在含铬(VI)培养基中同时接种Chr-9和Chr-43时,Chr-9促进菌株Chr-43的生长,并提高菌株去除铬(VI)的效率. 研究表明,pH、温度、阴离子和环境中的其它生物对铬(VI)的去除有明显影响. 图7 表1 参24
Abstract:
For understanding the effect of environment factors on the removal of chromate by bacterial strains and to provide chromate-removing strains for the bioremediation of environments contaminated by chromate, two Gram-positive bacterial strains designated as Chr-9 and Chr-43 respectively, capable of removing Cr (VI) in LB medium, were isolated from sludge collected from one tannery plant in Binzhou, Shandong, China. Based on 16S rRNA genes and phenotype characteristics, strain Chr-9 and Chr-43 were identified as Rhodococcus and Exiguobacterium strains, respectively. The optimal temperature and pH for Cr (VI)-removal by the two strains were 35 ℃ and pH 7.0, respectively. The removal of Cr (VI) was enhanced by supplementation of 0.1~0.5 mol/L NO3-, SO42-, and Cl- for strain Chr-9 and by supplementation of 0.1~0.2 mol/L NO3-, SO42, and Cl- for strain Chr-43. The growth and Cr (VI)-removal rate of strain Chr-43 were both enhanced by co-cultivation with strain Chr-9. These results indicated that the removal of chromate by bacterial strains could be affected by temperature, pH, negative ions, and other co-cultured microbes. Fig 7, Tab 1, Ref 24

参考文献/References:

Lan SG (兰嗣国), Yin HM (殷惠民), Di YA (狄一安), Ren JZ (任剑璋). Detoxication techniques for chromium slag. Res Environ Sci (环境科学研究), 1998, 11 (3): 53~56
Jiang L(江澜). The application of microorganisms to treatment of pollution by chromium. J Chongqing Tech Bus Univ Nat Sci (重庆工商大学学报自然科学版), 2006, 23 (2): 132~135
Brinton HP, Frasier ES, Koven AL. Morbidity and mortality experience among chromate workers. Public Health Rep, 1952, 67: 835~847
Bidstrup PL, Case RAM. Carcinoma of the lung in workmen in the biochromates-producing industry in Great Britain. Br J Ind Med, 1956, 13: 260~264
Pan C (潘翠). Simultaneous removal of Cr (Ⅵ) and phenol in a consortium bacterial culture: [Master Degree Thesis]. Changsha: Hunan University (长沙: 湖南大学), 2008
Gibb HJ, Lee PS, Pinsky PF. Lung cancer among workers in chromium chemical production. Am J Ind Med, 2000, 38: 115~126
Luli GW, Talnagi JW, Strohl WR. Hexavalent chromiun-resistant bacteria isolated from river sediments. Appl Environ Microb, 1983, 46: 846~854
He BY (何宝燕),Yin H (尹华), Peng H (彭辉), Ye JS (叶锦韶), Yang F (杨峰),Qin MH (秦华明), Zhang N (张娜). Cell physiology metabolism and morphology study of chromium biosorption by yeast. Environ Sci (环境科学), 2007, 28: 194~198
Srivastava J, Chandra H, Tripathi K, Naraian R, Sahu RK. Removal of chromium (VI) through biosorption by the Pseudomonas spp. isolated from tannery effluent. J Basic Microb, 2008, 48: 135~139
Cheng G, Li X. Bioreduction of chromium (VI) by Bacillus sp isolated from soils of iron mineral area. Eur J Soil Biol, 2009, 45: 483~487
Alam MZ, Malik A. Chromate resistance, transport and bioreduction by Exiguobacterium sp. ZM-2 isolated from agricultural soil irrigated with tannery effluent. J Basic Microb, 2008, 48: 416~420
Zhu WJ (朱文杰). Mechanism of Cr(VI) reduction with Leucobacter sp. CRB1 and its application in detoxification of chromite oprocessing residue: [PhD Theosis]. Changsha: Central South University (长沙: 中南大学), 2007
Quan X, Tan H, Zhao Y, Hu Y. Detoxification of chromium slag by chromate resistant bacteria. J Hazard Mater, 2006, 137: 836~841.
Kourtev PS, Nakatsu CH, Konopka A. Responses of the anaerobic bacterial community to addition of organic C in chromium (Ⅵ)-and iron (Ⅲ)-amended microcosms. Appl Environ Microb, 2006, 72 (1): 628~637
Bankar AV, Kumar AR, Zinjarde SS. Removal of chromium (VI) ions from aqueous solution by adsorption onto two marine isolates of Yarrowia lipolytica. J Hazard Mater, 2009, 170: 487~494
Ye JS, Yin H, Mai BX, Peng H, Qin HM, He BY, Zhang N. Biosorption of chromium from aqueous solution and electroplating wastewater using mixture of Candida lipolytica and dewatered sewage sludge. Bioresourc Technol, 2010, 101: 3893~3902
Nancharaiah YV, Dodge C, Venugopalan VP, Narasimhan SV, Francis AJ. Immobilization of Cr(VI) and its reduction to Cr(III) phosphate by granular biofilms comprising a mixture of microbes. Appl Environ Microb, 2010, 76: 2433~2438
Dong XZ (东秀珠), Cai MY (蔡妙英). System Manual of Common Bacterial Identification. Beijing: Science Press (北京: 科学出版社), 2001
Sambrook J, Russell DW. Molecular Cloning: A Laboratore Manual. Cold Spring Harbor Laboratory Press, 2002. 26~278
Hiorns WD, Methe BA, Nierzwicki-Baucer SA, Zehr JP. Bacterial diversity in Adiondack mountain lakes as revealed by 16S rRNA gene sequences. Appl Environ Microb, 1997, 63: 2957~2960
Sun JQ, Xu L, Tang YQ, Liu WQ, Chen FM, Wu XL. Degradation of pyridine by one Rhodococcus strain in the presence of chromium (VI) or phenol. J Hazard Mater, 2011, 191: 62~68
Sun JQ (孙纪全), Xu L (徐莲), Tang YQ (汤岳琴), Wu XL (吴晓磊), Chen FM (陈福明). Degradation characteristics of pyridine and phenol by Rhodococcus sp. Chr-9. Chin J Appl Environ Biol (应用与环境生物学报), 2012, 18 (4): 647~650
Yang YR (杨宇容), Xu LH (徐丽华), Duan RL (段若玲), Jin X (金湘), Wang QM (王启兰), Jiang CL (姜成林). A study on isolation methods of rare acinomycetes. J Yunnan Univ Nat Sci (云南大学学报自然科学版), 1997, 19 (4): 403~408
Prescott LM, Harley JP, Klein DA. Microbiology. 5th ed. New York: McGraw?Hill Companies, 2002

相似文献/References:

[1]黄天培,张巧铃,潘洁茹,等.高效还原铬的苏云金芽胞杆菌菌株筛选[J].应用与环境生物学报,2010,16(06):879.[doi:10.3724/SP.J.1145.2010.00879]
 HUANG Tianpei,ZHANG Qiaoling,PAN Jieru,et al.Screening of Bacillus thuringiensis with High Chromium Reducing Capacity[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):879.[doi:10.3724/SP.J.1145.2010.00879]

备注/Memo

备注/Memo:
“973”计划项目(No. 2007CB815601)和深圳市科技计划项目(No. JC200903170472A)资助 Supported by the National Basic Research Program of China (“973” Program, No. 2007CB815601), and the Science & Technology Plan of Shenzhen, China (No. JC200903170472A)
更新日期/Last Update: 2012-12-28