|本期目录/Table of Contents|

[1]于寒颖,杨慧.石油烃降解酶及其基因的结构、功能和表达调控[J].应用与环境生物学报,2012,18(06):1066-1074.[doi:10.3724/SP.J.1145.2012.01066]
 YU Hanying,YANG Hui.Structure, Function and Expression Regulation of Hydrocarbon-degrading Enzymes and Their Encoding Genes[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):1066-1074.[doi:10.3724/SP.J.1145.2012.01066]
点击复制

石油烃降解酶及其基因的结构、功能和表达调控()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年06期
页码:
1066-1074
栏目:
综述
出版日期:
2012-12-25

文章信息/Info

Title:
Structure, Function and Expression Regulation of Hydrocarbon-degrading Enzymes and Their Encoding Genes
作者:
于寒颖 杨慧
(东北石油大学石油工程学院提高油气采收率教育部重点实验室 大庆 163318)
Author(s):
YU Hanying YANG Hui
(Enhanced Oil and Gas Recovery Key Laboratory of Ministry of Education, Department of Petroleum Engineering, Northeast Petroleum University, Daqing 163318, Heilongjiang, China)
关键词:
石油微生物加氧酶苯酰琥珀酸合酶乙苯脱氢酶基因表达调控
Keywords:
petroleum microorganism hydrocarbon oxydase benzoylsuccinate sythase ethylbenzene dehydrogenase gene regulation
分类号:
Q939.97 : Q78
DOI:
10.3724/SP.J.1145.2012.01066
文献标志码:
A
摘要:
研究烃降解酶及其基因是进行石油微生物分子检测和工程菌构建的重要基础. 本文对目前烃降解酶及其基因的结构、功能和调控机制的最新研究进展进行了总结. 催化烷烃好氧降解的起始酶有几类加氧酶,膜整合甲烷单加氧酶、萘-1,2-双加氧酶和异丙苯双加氧酶的晶体结构已经被解析. 烷基或芳基琥珀酸合酶催化烃厌氧代谢的主要起始反应,而Azoarcus sp.乙苯厌氧代谢起始反应由乙苯脱氢酶催化. 在细菌中,烃代谢相关基因主要通过形成操纵子进行表达调控,基因转录受烃或类似物诱导,并受细胞全局调控. 一些微生物由于存在多种烃代谢途径而可能具有复杂的基因调控机制. 此外,生态学研究表明,环境中烃降解基因的诱导动态与实验室内纯培养分析不同. 在分析石油降解工程菌构建解决问题的基础上,提出了烃代谢综合调控和环境中相关酶及基因诱导研究的重要性,并对未来烃降解酶及其基因在有毒物降解理论研究和生物修复上的应用进行了展望. 图3 参50
Abstract:
The research on the enzymes and genes involving in hydrocarbon biodegradation is the important basis of molecular assay of petroleum microbes and construction of the engineering strains. In this review, the recent advances of structures, functions and regulations of hydrocarbon-degrading enzymes and the corresponding genes were summarized. The first enzymes in the aerobic metabolism pathways of hydrocarbon included several types of oxydases. Among them, the crystal structures of particulate methane monooxygenase protomer, the terminal oxygenase component of cumene dioxygenase and naphthalene dioxygenase were reported. The aryl-succinate or alkyl-succinate synthase were the first basic enzymes in the anaerobic transformation of hydrocarbon while ethylbenzene dehydrogenase catalyzed the initial reaction of anaerobic degradation of ethylbenzene in Azoarcus sp. For bacteria, the hydrocarbon-degrading genes consisting of operons and their transcriptions were induced by hydrocarbon or analogues and limited by global cell control. Some microorganisms possessed the complicated hydrocarbon-degrading regulations which were consistent with their various hydrocarbon metabolism pathways. In addition, the related study on ecology indicated that the induction process of genes involving in the hydrocarbon degradation in the environment were different from that of the culture in the laboratory. Based on the analysis of the unsolved questions of construction the hydrocarbon-degrading engineering bacteria, the significance of research on the comprehensive regulation of hydrocarbon metabolism in the cells and the induction of the related enzymes and genes in the environment was proposed. The application of the enzymes and genes, involving in hydrocarbon biodegradation, for the theoretic research on toxicant degradation and bioremediation in the future was prospected. Fig 3, Ref 50

参考文献/References:

1 Widdel F, Boetius A, Rabus R. Anaerobic biodegradation of hydrocarbons including methane. Prokaryotes, 2006, 2: 1028~1049
2 Zhao Y (赵渝). The protemic research of aromatic hydrocarbon biodegradation pathway: [Doctor Degree Dissertation]. Shanghai: Eastchina Normal University (上海: 华东师范大学), 2007
3 Ollivier B, Magot M. Petroleum Microbiology. Washington, D C: ASM Press, 2005
4 Lu J (陆健). Isolaton and characterizations of a high-efficiency alkane-degrading bacterium XCZ and functional analysis of its monooxygenase genes alkB: [Master Degree Dissertatio]. Nanjing: Nanjing Agricultural University (南京: 南京农业大学), 2007
5 Mason JR, Cammack R. The electron-transport proteins of hydroxylating bacterial dioxygenases. Annu Rev Microbiol, 1992, 46: 277~305
6 Zhang J (章俭), Xia CG (夏春谷). Studies of structure and function of aromatic hydrocarbon dioxygenases. Prog Chem (化学进展), 2004, 16 (1): 116~122
7 Lieberman RL, Rosenzweig AC. Crystal structure of particulate methane monooxygenase [M/OL]. www.umich.edu/~icbic/Abstracts/371805-1.pdf
8 Ferraro DJ, Okerlund AL, Mowers JC, Ramaswamy S. Structural basis for regioselectivity and stereoselectivity of product formation by naphthalene 1,2-dioxygenase. J Bacteriol, 2006, 188 (19): 6986~6994
9 Dong XS, Fushinobu S, Fukuda E, Terada T, Nakamura S, Shimizu K, Nojiri H, Omori T, Shoun H, Wakagi T. Crystal structure of the terminal oxygenase component of cumene dioxygenase from Pseudomonas fluorescens IP01. J Bacteriol, 2005, 187 (7): 2483~2490
10 van Beilen JB, Smits THM, Roos FF, Brunner T, Balada SB, Röthlisberger M, Witholt B. Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases. J Bacteriol, 2005, 187 (1): 85~91
11 Li X (黎霞), Cheng L (承磊), Deng Y (邓宇), Zhang H (张辉). Advances in anaerobic of biodegradation of hydrocarbon. Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (2): 283~289
12 Heider J, Spormann AM, Beller HR, Widdel F. Anaerobic bacterial metabolism of hydrocarbons. FEMS Microbiol Rev, 1999, 22: 459~473
13 Boll M, Fuchs G, Heider J. Anaerobic oxidation of aromatic compounds and hydrocarbons. Curr Opin Chem Biol, 2002, 6: 604~611
14 Leuthner B, Leutwein C, Schulz H, Hörth P, Haehnel W, Schiltz E, Schägger H, Heider J. Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalyzing the first step in anaerobic toluene metabolism. Mol Microbiol, 1998, 28 (3): 615~628
15 Krieger CJ, Roseboom W, Albracht SPJ, Spormann AM. A Stable organic free radical in anaerobic benzylsuccinate synthase of Azoarcus sp. strain T. J Biol Chem, 2001, 276 (16): 12924~12927
16 Kniemeyer O, Heider J. Ethylbenzene dehydrogenase, a novel hydrocarbon-oxidizing molybdenum/iron-sulfur/heme enzyme. J Biol Chem, 2001, 276 (24): 21381~21386
17 Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme. J Bacteriol, 2001, 183 (15): 4536~4542
18 Atlas RM ed; Huang DF (黄第藩), Tan S (谭实), Yang WK (杨文宽), et al. trans. 石油微生物学. Beijing: Petroleum Industry Press (北京: 石油工业出版社), 1991. 4, 266, 268, 338, 369
19 van Beilen JB, Li Z, Duetz WA, Smits THM, Witholt B. Diversity of alkane hydroxylase systems in the environment. Oil & Gas Sci & Technol-Rev IFP, 2003, 58 (4): 427~440
20 Panke S, Meyer A, Huber CM, Witholt B, Wubbolts MG. An alkane-resposive expression system for the production of fine chemicals. Appl & Environ Microbiol, 1999, 65 (6): 2324~2332
21 van Beilen JB, Panke S, Lucchini S, Franchini AG, Röthlisberger M, Witholt B. Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes. Microbiology, 2001, 147: 1621~1630
22 van Beilen JB, Wubbolts MG, Witholt B. Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation, 1994, 5: 161~174
23 Wentzel A, Ellingsen TE, Kotlar HK, Zotchev SB, Throne-Holst M. Bacterial metabolism of long-chain n-alkanes. Appl Microbiol Biotechnol, 2007, 76: 1209~1221
24 Ramos JL, Marqués S. Transcriptional control of the Pseudomonas Tol plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu Rev Microbiol, 1997, 51: 341~373
25 Hermuth K, Leuthner B, Heider J. Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172. Arch Microbiol, 2002, 177: 132~138
26 Kube M, Heider J, Amann J, Hufnagel P, Kuhner S, Beck A, Reinhardt R, Rabus R. Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1. Arch Microbiol, 2004, 181: 182~194
27 Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. J Bacteriol, 2001, 183 (23): 6763~6770
28 Rabus R, Kube M, Beck A, Friedrich W, Richard R. Genes involved in the anaerobic degradation of ethylbenzene in a denitrifying bacterium, strain EbN1. Arch Microbiol, 2002, 178 (6): 506~516
29 Kühner S, Wöhlbrand L, Fritz I, Wruck W, Hultschig C, Hufnagel P, Kube M, Reinhardt R, Rabus R. Substrate-dependent regulation of anaerobic degradation pathways for toluene and ethylbenzene in a denitrifying bacterium, strain EbN1. J Bacteriol, 2005, 187 (4): 1493~1503
30 Wu FX (武凤霞). Isolation of phenanthrene degrading bacteria from oil-contaminated soil and their effects on phenanthrene degradation: [Master Degreee Dissertation]. Xi’an: Northwest University (西安: 西北大学), 2006
31 Laurie AD, Lloyd-Jones G. The phn genes of Burkholderia sp. strain RP007 constitute a divergent gene cluster for polycyclic aromatic hydrocarbon catabolism. J Bacteriol, 1999, 181 (2): 531~540
32 Kiyohara H, Torigoe S, Kaida N, Asaki T, Lida T, Hayashi H, Takizawa N. Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82. J Bacteriol, 1994, 176: 2439~2443
33 Tani A, Ishige T, Sakai Y, Kato N. Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp. M-1. J Bacteriol, 2001, 183 (5): 1819~1823
34 Duetz WA, Marqués S, Wind B, Ramos JL, van Andel JG. Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWW0 under various conditions of nutrient limitation in chemostat culture. Appl & Environ Microbiol, 1996: 601~606
35 Santos PM, Sá-Correia I. Characterization of the unique organization and co-regulation of a gene cluster required for phenol and benzene catabolism in Pseudomonas sp. M1. J Biotechnol, 2007, 131 (4): 371~378
36 Sze CC, Bernardo LMD, Shingler V. Integration of global regulation of two aromatic-responsive σ54-dependent systems: a common phenotype by different mechanisms. J Bacteriol, 2002, 184 (3): 760~770
37 Shinoda Y, Sakai Y, Uenishi H, Uchihashi Y, Hiraishi A, Yukawa H, Yurimoto H, Kato N. Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1. Appl & Environ Microbiol, 2004, 70 (3): 1385~1392
38 Aitken CM, Jones DM, Larter SR. Anaerobic hydrocarbon biodegradation in deep subsurface oil reservoirs. Nature, 2004, 431 (16): 291~294
39 Sun HB (孙海波). Isolation, identification and characteristics of PAHs-degrading bacteria and preliminary study on catechol 2,3-dioxygenase: [Master Degree Dissertation]. Jinan: Shandong University (济南: 山东大学), 2009
40 Xie DP (谢丹平), Yin H (尹华), Peng H (彭辉), Ye JS (叶锦韶), Zhang N (张娜). Degradation of crude oil by mixed culure. Chin J Appl Environ Biol (应用与环境生物学报), 2004, 10 (2): 210~214
41 Guo N (郭娜), Li ZM (李志敏), Ye Q (叶勤). Screen and identification of alkane degrading bacteria and characteristics of a predominant strain. Chin J Appl Environ Biol (应用与环境生物学报), 2011, 17 (4): 572~576
42 Parisi VA, Brubaker GR, Zenker MJ, Zenker MJ, Prince RC, Gieg LM, da Silva MLB, Alvarez PJJ, Suflita JM. Field metabolomics and laboratory assessments of anaerobic intrinsic bioremediation of hydrocarbons at a petroleum-contaminated site. Microb Biotechnol, 2009, 2 (2): 202~212
43 Piskonen R, Nyyssönen M, Itävaara M. Evaluating the biodegradation of aromatic hydrocarbons by monitoring of several functional genes. Biodegradation, 2008, 19: 883~895
44 Berthe-Corti L, Fetzner S. Bacterial metabolism of n-alkanes and ammonia under oxic, suboxic and anoxic conditions. Acta Biotechnol, 2002, 22 (3~4): 299~336
45 Kim H, Jaffe PR. Degradation of toluene by a mixed population of archetypal aerobes, microaerophiles, and denitrifiers: laboratory sand column experiment and multispecies biofilm model formulation. Biotechnol & Bioengin, 2008, 99 (2): 290~301
46 Leutwein C, Heider J. Succinyl-CoA: (R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria. J Bacteriol, 2001, 183 (14): 4288~4295
47 Kniemeyer O, Heider J. (S)-1-phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ethylbenzene catabolism. Arch Microbiol, 2001, 176: 129~135
48 Sota M, Yano H, Ono A, Miyazaki R, Ishii H, Genka H, Top EM, Tsuda M. Genomic and functional analysis of the IncP-9 naphthalene-catabolic plasmid NAH7 and its transposon Tn4655 suggests catabolic gene spread by a tyrosine recombinase. J Bacteriol, 2006, 188 (11): 4057~4067
49 Zhou L (周蕾), Mbadinga SM, Wang LY (王立影), Liu JF (刘金峰), Yang SZ (杨世忠), Mu BZ (牟伯中). Recent progress in metabolites formed during anaerobic biodegradation of petroleum hydrocarbons. Chin J Appl Environ Biol (应用与环境生物学报), 2011, 17 (4): 607~613
50 Zhou DP (周德平). Isolation, identification, degradation charaeteristics of and cloning, expression of catechol 2,3-dioxygenase gene of phenanthrene-degrading bacteria: [Master Degree Dissertation]. Hangzhou: Zhejiang University (杭州: 浙江大学), 2003

相似文献/References:

[1]许 颖** 马德胜 宋文枫 魏小芳.16S rDNA高通量测序技术分析油藏微生物多样性?[J].应用与环境生物学报,2016,22(03):409.[doi:10.3724/SP.J.1145.2015.09003]
 XU Ying**,MA Desheng,SONG Wenfeng & WEI Xiaofang.16S rDNA-assisted high-throughput sequencing analysis of microbial diversity in oil reservoirs*[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):409.[doi:10.3724/SP.J.1145.2015.09003]

备注/Memo

备注/Memo:
国家自然科学基金项目(No. 51104031)资助 Supported by the National Natural Science Foudation of China (No. 51104031)
更新日期/Last Update: 2012-12-28