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[1]刘茜,张娟,陈坚,等.喷雾冷凝法制备高性能乳酸菌微胶囊[J].应用与环境生物学报,2012,18(05):824-830.[doi:10.3724/SP.J.1145.2012.00824]
　LIU Qian,ZHANG Juan,CHEN Jian,et al.Microencapsulation of Lactic Acid Bacteria with Improved Performance by Spray-congealing[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):824-830.[doi:10.3724/SP.J.1145.2012.00824]
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喷雾冷凝法制备高性能乳酸菌微胶囊()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年05期
页码:
824-830
栏目:
技术与方法
出版日期:
2012-10-25

文章信息/Info

Title:
Microencapsulation of Lactic Acid Bacteria with Improved Performance by Spray-congealing
作者:
刘茜; 张娟; 陈坚; 张岳玲; 王连艳; 王淼; 马光辉; 堵国成
（1江南大学生物工程学院 无锡 214122）
（2江南大学工业生物技术教育部重点实验室 无锡 214122）
（3中国科学院过程工程研究所生化工程国家重点实验室 北京 100190）
（4江南大学食品科学与技术国家重点实验室 无锡 214122）
（5江南大学食品学院 无锡 214122）
Author(s):
LIU Qian;  ZHANG Juan;  CHEN Jian;  ZHANG Yueling;  WANG Lianyan;  WANG Miao;  MA Guanghui;  DU Guocheng
(1School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China)
(2Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China)
(3National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China)
(4State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China)
关键词:
微胶囊; 乳酸菌; 喷雾冷凝法; 变性淀粉; 胃肠道存活率
Keywords:
microencapsulation;  Lactobacillus;  spray-congealing;  resistant starch;  survival rate in human gastrointestinal tract
分类号:
TQ460.6 : R378.99+2
DOI:
10.3724/SP.J.1145.2012.00824
文献标志码:
A
摘要:
活菌制剂进入动物消化道后由于经受胃酸、胆盐以及消化酶等的作用而造成活性丧失及肠道定殖困难是目前乳杆菌制剂应用的瓶颈问题. 本研究通过喷雾冷凝法制备干酪乳杆菌微胶囊以增强乳杆菌在人体的益生功能. 选用不同浓度的海藻酸钠溶液与氯化钙溶液作为壁材，考察喷雾冷凝法制备乳杆菌微胶囊的包埋率、胃肠道释放等情况. 通过比较微胶囊的包埋率和粒径分布的效果后，选择海藻酸钠浓度为2%、氯化钙浓度为3%的芯壁材（包埋率最高达95.80%）制备干酪乳杆菌微胶囊. 初始活菌数为109 cfu/mL的乳酸菌微胶囊经模拟人体胃液处理3 h后，仅有24.17%的活菌溢出，证明微胶囊处理后的乳杆菌能够抵抗较低pH值及胃蛋白酶的作用. 之后将微胶囊置入模拟人体肠液中，60 min后微胶囊释放率达到84.22%，表明上述方法制备的乳杆菌微胶囊能够在肠道中释放定殖并发挥其益生作用. 进一步研究表明，添加变性淀粉作为益生元，能够增强乳酸菌微胶囊的耐酸性能，并且在肠液中释放后活菌出现继续增殖的现象. 上述研究结果为选择高效保护剂应用喷雾冷凝法制备乳酸菌微胶囊奠定了研究基础. 图4 表2 参34
Abstract:
The microencapsulation containing Lactobacillus casei by spray-congealing was investigated. The optimal concentrations of sodium alginate and CaCl2 obtained by comparing the encapsulation efficiencies and particle size distributions of microcapsule were 2% and 3%, respectively. Meanwhile, the encapsulation efficiency reached up to 95.80%. Furthermore, the microcapsules with 109 cfu/mL were digested in simulated human gastric juice for 3 h, and only 24.17% of cells escaped from microcapsule, suggesting that the microencapsulation may protect the cells against low pH and pepsin. Subsequently, microcapsules were transferred into the simulated human intestinal juice, and 84.22% of the cells in microcapsules released after treating for 60 min. These results indicated the advantages of spray-congealed microcapsules with proper releasing area and health-promoting effects. Resistant starch was then added as prebiotic based on this formula, and large amounts of viable cells were obtained after 60 min in simulated human intestinal juice in this case. This research demonstrated a novel method that microencapsulation by spray-congealing with protective agent such as resistant starch significantly improved the survival of L. casei during extreme conditions such as the human gastro-intestinal tract, thus enlighten us to exploit its industrial application in the future. Fig 4, Tab 2, Ref 34

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备注/Memo

备注/Memo:
国家自然科学基金重点项目（No. 20836003）、国家自然科学基金项目（No. 30900013）和江南大学自主科研计划面上项目（JUSRP 21009）资助
更新日期/Last Update: 2012-10-26