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[1]王志勇,方治伟,江雪飞,等.空心莲子草入侵对土壤AM真菌生物量和群落结构的影响——以湖北省典型区域为例[J].应用与环境生物学报,2013,19(01):105-112.[doi:10.3724/SP.J.1145.2013.00105]
 WANG Zhiyong,FANG Zhiwei,JIANG Xuefei,et al.Effect of Alligator Weed Invasion on Soil Arbuscular Mycorrhizal Fungal Biomass and Community Structure of Representative Regions in Hubei, China[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):105-112.[doi:10.3724/SP.J.1145.2013.00105]
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空心莲子草入侵对土壤AM真菌生物量和群落结构的影响——以湖北省典型区域为例()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年01期
页码:
105-112
栏目:
研究论文
出版日期:
2013-02-25

文章信息/Info

Title:
Effect of Alligator Weed Invasion on Soil Arbuscular Mycorrhizal Fungal Biomass and Community Structure of Representative Regions in Hubei, China
作者:
王志勇方治伟江雪飞郑慧袁继红郑世学
(1华中农业大学农业微生物学国家重点实验室,微生物农药国家工程研究中心 武汉 430070)
(2咸宁职业技术学院 咸宁 437100)
Author(s):
WANG ZhiyongFANG ZhiweiJIANG XuefeiZHENG HuiYUAN JihongZHENG Shixue
(1State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China)
(2Xianning Vocational Technical College, Xianning 437100, Hubei, China)
关键词:
空心莲子草丛枝菌根真菌FAMEsDGGE群落结构马尼拉草坪系统发育树
Keywords:
Alternanthera philoxeroides arbuscular mycorrhizal fungi FAMEs DGGE community structure Zoysiarass matrella lawn phylogenetic tree
分类号:
Q948.12+2.3
DOI:
10.3724/SP.J.1145.2013.00105
文献标志码:
A
摘要:
土壤丛枝菌根(Arbuscular mycorrhizal,AM)真菌具有维持植被稳定性和多样性的重要作用. 为了解空心莲子草入侵过程中与AM真菌的相互作用,采用脂肪酸甲酯(FAMEs)和基于18S rDNA的PCR-DGGE方法对湖北咸宁、仙桃和武汉三地空心莲子草(Alternanthera philoxeroides)入侵前后土壤AM真菌的生物量和群落结构进行了比较分析. 结果发现,基于FAMEs表征的AM真菌生物量在空心莲子草入侵后变化不大,仅仙桃地区显著增加(P<0.05). 尽管DGGE图谱显示咸宁、仙桃和武汉三地AM真菌优势类群的群落结构均发生了明显演替,但群落间物种组成差异的多样性指数βWH在2.55-2.73之间、群落多样性指数H′在1.22-1.38之间,变化均不大. 结果表明,空心莲子草入侵所形成的植物更替明显改变了AM真菌优势类群的结构,但对AM真菌的生物量和多样性影响很小.
Abstract:
Soil arbuscular mycorrhizal fungi (AMF) play a crucial role in plant biodiversity and ecosystem variability. However, little was known about the interaction between AMF and alligator weed (Alternanthera philoxeroides) during the weed invasion. Soil samples, collected from Xianning, Xiantao and Wuhan, Hubei Province, were used to investigate the response of AMF biomass under alligator weed invasion using fatty acid methyl ester (FAMEs), and the AMF community structure using PCR-denaturing gradient gel electrophoresis (DGGE) for 18S rDNA. The AMF biomass indicated by FAMEs had no significant increase except for soil sample from Xiantao (P<0.05) after alligator weed invasion. Soil AMF community structure of dominant population represented by DGGE shifted significantly under alligator weed invasion. However, both the βWH (diversity index of species composition in the community, ranging from 2.55 to 2.73) and the H′ (diversity index of the community, ranging from 1.22 to 1.38) had no significant change. In conclusion, alligator weed invasion resulted in obvious change of the AMF community structure, but had little impact on the AMF biomass and diversity.

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备注/Memo

备注/Memo:
中央高校基本科研业务费专项资金(2009PY013)资助 Supported by the Fundamental Research Fund for the Central Universities of China (No. 2009PY013)
更新日期/Last Update: 2013-02-26