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[1]刘方春,邢尚军,马海林,等.PGPR生物肥对甜樱桃(Cerasus pseudocerasus)根际土壤生物学特征的影响[J].应用与环境生物学报,2012,18(05):722-727.[doi:10.3724/SP.J.1145.2012.00722]
 LIU Fangchun,XING Shangjun,MA Hailin,et al.Effect of PGPR Fertilizer on Biological Characteristics in Cerasus pseudocerasus Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):722-727.[doi:10.3724/SP.J.1145.2012.00722]
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PGPR生物肥对甜樱桃(Cerasus pseudocerasus)根际土壤生物学特征的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年05期
页码:
722-727
栏目:
研究论文
出版日期:
2012-10-25

文章信息/Info

Title:
Effect of PGPR Fertilizer on Biological Characteristics in Cerasus pseudocerasus Rhizosphere
作者:
刘方春邢尚军马海林杜振宇马丙尧陈波杜秉海
(1山东省林业科学研究院,山东省森林植被生态修复工程技术研究中心 济南 250014)
(2山东农业大学生命科学学院 泰安 271018)
Author(s):
LIU Fangchun XING Shangjun MA Hailin DU Zhenyu MA Bingyao CHEN Bo DU Binghai
(1Shandong Academy of Forest, Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Jinan 250014, China)
(2College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, China)
关键词:
甜樱桃生物肥鸡粪根际根系植物根际促生细菌
Keywords:
sweet cherry bio-fertilizer decomposed chicken manure rhizosphere root plant growth-promoting rhizobacterium
分类号:
S144.1 : S662.506
DOI:
10.3724/SP.J.1145.2012.00722
文献标志码:
A
摘要:
利用保绿法和萝卜子叶增重法从7年生甜樱桃[Cerasus pseudocerasus (Lindl.) G. Don]根际土壤中,筛选具有促生作用的植物根际促生细菌YT-3(PGPR),以发酵好的鸡粪(DCM)为吸附载体制成甜樱桃专用的PGPR生物肥料(YMF),对比研究了YMF、普通生物肥(NMF)和DCM对樱桃根系和根际土壤生物学特征的影响. 结果表明:YMF显著增加了根际土壤中细菌数量和微生物总量,真菌数量明显减少,但对放线菌数量影响差异不显著. YMF处理根系活力分别比NMF、DCM和CK提高了22.49%、13.25%和15.33%. PGPR生物肥料对樱桃根系生长和构建影响显著,YMF处理0~40 cm土壤剖面中根系重量尤其是毛细根重量显著增加. 同NMF处理相比,YMF处理根际土壤的pH降低8.61%,阳离子代换能力显著提高. 此外,YMF处理显著增加了根际土壤中养分离子的有效性,速效磷和有效钾含量分别增加17.21%和9.56%,但碱解氮含量差异不显著. 因此,PGPR生物肥的施用在一定程度上改善了根际土壤的生态环境,提高了根际土壤中养分离子的有效性和养分保持能力,提高了根系活力,促进了表层土壤中(主要为0~40 cm)根系尤其是毛细根的生长. 图2 表3 参17
Abstract:
The aim of this research was to determine the effects of plant growth-promoting rhizobacteria (PGPR) fertilizer on the biological characteristics, root activity, growth and construction of Cerasus pseudocerasus (Lindl.) G. Don (sweet cherry). To prepare sweet cherry biological-fertilizer (YMF), dominant bacteria YT-3, a type of PGPR, was extracted from rhizosphere soil of C. pseudocerasus trees by keeping green method and radish cotyledon weight increase method. Sweet cherry biological-fertilizer was prepared by compounding YT-3 and decomposed chicken manure (DCM). The effects of YMF, normal biological fertilizer (NMF) and DCM on biological characteristics of C. pseudocerasus rhizosphere soil were studied in Yiyuan sweet cherry orchards. And the effects of YMF, DCM and NMF on the root activity, growth and construction of C. pseudocerasus were also investigated. The results showed that YMF increased the amount of bacteria and total microorganism amount in rhizosphere soil of C. pseudocerasus, but the fungus amount was decreased significantly. However, YMF had no influence on actinomycetes amount in rhizosphere soil. Root activity in YMF was significantly increased by 15.33%, 22.49% and 13.25% than that in CK, NMF and DCM, respectively. YMF had great influence on the root growth and construction of C. pseudocerasus. Root weight, especially fine root weight in 0~40 soil profile of YMF was significantly increased. Rhizosphere soil pH value in YMF decreased by 8.61% than that in NMF. In addition, YMF significantly enhanced available phosphorus and potassium contents by 17.21% and 9.56%, respectively, compared to that in NMF. However, it had no effects on available nitrogen content in sweet cherry rhizosphere soil. Moreover, PGPR fertilizer increased the cation exchange capacity of rhizosphere soil, which benefited the nutrient retain and uptake of C. pseudocerasus. As a result, plant growth-promoting rhizobactera biological-fertilizer benefited the ecological environment of sweet cherry rhizosphere soil, improved nutrient availability and cation exchange capacity, increased the root activity and benefited the root growth, especially fine root growth in top soil (mainly 0~40 cm). Fig 2, Tab 3, Ref 17

参考文献/References:

1 Xing SJ (邢尚军), Liu FC (刘方春), Ma BY (马丙尧), Du ZY (杜振宇), Ma HL (马海林), Duan CH (段春华). Storage property, calcium fractions, and cell ultrastructure in sweet cherry as affected by calcium. Ecol & Environ Sci (生态环境学报), 2010, 19 (9): 2091~2096
2 Fang QX, Yu Q, Wang EL, Chen YH, Zhang GL, Wang J, Li LH. Soil nitrate accumulation, leaching and crop nitrogen use as influenced by fertilization and irrigation in an intensive wheat maize double cropping system in the North China Plain. Plant Soil, 2006, 284: 335~350
3 Liu FC (刘方春), Xing SJ (邢尚军), Duan CH (段春华), Du ZY (杜振宇), Ma HL (马海林), Ma BY (马丙尧). Nitrate nitrogen leaching and residue of humic acid fertilizer in field soil. Environ Sci (环境科学), 2010, 31 (7): 1619~1624
4 Zheng SX, Nie J, Dai PA, Zheng YJ. Nitrogen recovery and nitrate leaching of controlled release nitrogen fertilizer in irrigated paddy soil. Agr Ecosyst Environ, 2004, 5 (3): 1~10
5 Liu FC (刘方春), Xing SJ (邢尚军), Ma HL (马海林), Du ZY (杜振宇), Ma BY (马丙尧), Duan CH (段春华), Chen B (陈波). Effects of biological organic fertilizer on biological characteristics, yield and quality of Zizyphus jujube Mill. Dongzao Trees. J Soil & Water Conserv (水土保持学报), 2010, 24 (6): 222~225
6 Norton JM. Carbonflow in the rhizosphere of Ponderosa pine seedlings. Soil Biol Biochem, 1990, 22 (4): 149~455
7 Liu HF (刘慧芬), Wang WW (王卫卫), Cao GL (曹桂林), Tang M (唐明). Effect of hydrogen on microbial population and enzyme activity in Robinia pseudoacacia rhizosphere soil. Chin J Appl Environ Biol (应用与环境生物学报), 2010, 16(4): 515~518
8 Jin ZZ (靳正忠), Lei JQ (雷加强), Li SY (李生宇), Xu XW (徐新文), Chang Q (常青). Variation in rhizosphere microbes of three shelter shrubs in drift desert hinterland in Xinjiang, China. Chin J Appl Environ Biol (应用与环境生物学报), 2010, 16 (6): 759~764
9 Zhou LH (周莉华), Li WJ (李维炯), Ni YZ (倪永珍). Effects of long-term application of EM biological-organic fertilizer on winter wheat production. Trans Chin Soc Agric Engin (农业工程学报), 2005, 21 (Suppl): 221~224
10 Yang SL (杨胜利), Liu HL (刘洪禄), Hao ZY (郝仲勇),Wu WY (吴文勇). Spatial distribution characteristics of cherry tree roots under border irrigation condition. Trans Chin Soc Agric Engin (农业工程学报), 2009, 25 (Suppl 1): 34~38
11 Jia ZH (贾志红), Yang ZP (杨珍平), Sun M (孙敏), Miao GY (苗果园). Influence of different fertilizers to crop rhizosphere microorganisms. Acta Agron Sin (作物学报), 2004, 30 (5): 491~495
12 Lu RK (鲁如坤). Analytical methods for soil and agro-chemistry. Beijing: China Agriculture Science and Technology Publishing House (北京: 中国农业科技出版社), 2000
13 Drever JI, Vance GF. Role of soil organic acids in mineral weathering process. In: Pittman ED, Lewan MD. Organic Acids in Geological Process. New York: Springer-Verlag, 1994. 138~161
14 Yang SJ (杨守军), Liu DX (刘德玺), Sun YB (孙玉波), Zhou J (周健), Liu CH (刘春生). Effects of root pruning on physio-chemical and biological characteristics of Winter Jujube tree (Zizyphus jujube Mill.) rhizosphere soil. J Soil & Water Conserv (水土保持学报), 2009, 23 (5): 215~218
15 Nye PH. On estimating the uptake of nut ritions solubilized near root s or other surfaces. J Soil Sci, 1984, 35: 439 ~ 445
16 Campbell R, Greaves MP. Anatomy and community structure of the rhizosphere. Lynch JM. The Rhizosphere. Chichester: John Wiley and Sons, 1990. 11~34
17 Cheng W, Zhang Q, Coleman DC, Carroll CR, Hoffman CA. Is available carbon limiting microbial respiration in the rhizosphere. Soil Biol Biochem, 1996, 28: 1283~1288

备注/Memo

备注/Memo:
“十一五”国家科技支撑计划重大专项(No. 2006BAD03A1505)和山东省科学技术发展计划项目(No. 2007GG2009007)资助
更新日期/Last Update: 2012-10-26