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[1]王莉,凌琪,汤利华,等.Methylobacterium sp. YAL-2对乙酰甲胺磷的降解特性[J].应用与环境生物学报,2012,18(03):438-443.[doi:10.3724/SP.J.1145.2012.00438]
 WANG Li,LING Qi,TANG Lihua,et al.Degradation Characteristics of Acephate by Methylobacterium sp. YAL-2[J].Chinese Journal of Applied & Environmental Biology,2012,18(03):438-443.[doi:10.3724/SP.J.1145.2012.00438]

Methylobacterium sp. YAL-2对乙酰甲胺磷的降解特性()




Degradation Characteristics of Acephate by Methylobacterium sp. YAL-2
(1安徽建筑工业学院环境与能源工程系 合肥 230022)
(2南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室 南京 210095)
WANG Li LING Qi TANG Lihua LI Shunpeng JIANG Jiandong
(1Department of Environment and Energy Engineering, Anhui University of Architecture, Hefei 230022, China )
(2Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China )
乙酰甲胺磷Methylobacterium sp. YAL-2降解特性系统发育分析农药残留去除
acephate Methylobacterium sp. YAL-2 degradation characterization phylogenetic analysis acephate residue removal
从有机磷生产厂家的下水道污泥中分离出一株对高浓度和低浓度乙酰甲胺磷都具有高效降解能力的寡营养菌YAL-2,根据形态、生理生化和16S rRNA基因系统发育分析,将菌株YAL-2鉴定为Methylobacterium sp. 降解特性实验表明,菌株YAL-2能利用乙酰甲胺磷为唯一碳源生长和降解;在添加了甲醇的无机盐培养基中,84 h可完全降解300 mg L-1乙酰甲胺磷,24 h将50 mg L-1和10 mg L-1乙酰甲胺磷降至非检测水平;4 d能完全去除100 mg L-1甲胺磷,5 d分别降解58.4%和40.6%的100 mg L-1乐果、敌敌畏. 小青菜农药残留去除实验显示,菌株YAL-2可在7 d内将乙酰甲胺磷和甲胺磷将至限量水平. 结果表明,将菌株YAL-2应用于保证果蔬等食品的食用安全是可行的.
It is very urgent to remove acephate residues in fruits, vegetables and other foods. A oligotrophic bacteria YAL-2, capable of high efficiently degrading acephate both at high concentration and low concentration, was isolated from the wastewater sludge of a pesticide factory which has produced organophosphorus pesticides for many years. Strain YAL-2 was identified preliminarily as Methylobacterium sp. according to its morphological observation, physiological and biochemical test, and phylogenetic analysis based on 16S rRNA gene sequence. The degradation experiments indicated that the strain YAL-2 could utilize acephate as its sole carbon source for growth. In medium MSM-2 added methanol, the strain YAL-2 could completely remove 300 mg L-1 acephate after 84 h under optimal conditions, and degraded low concentration of acephate (from 10 mg L-1 to 50 mg L-1) to an non-detectable level after 24 h. Moreover, YAL-2 also showed the ability of degrading 100% of 100 mg L-1 methamidophos in 4 days, 58.4% of 100 mg L-1 dimethoate and 40.6% of 100 mg L-1 dichlorvos in 5 days. Spraying the strain YAL-2 could degrade acephate or methamidophos in pakchoi leaves to below the detectable level in safety interval of 7 days. These results suggested that it was feasible for applying the strain YAL-2 to ensure the edible safety of the fruits, vegetables and other foods.


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国家水体污染控制与治理科技重大专项(No. 2008ZX07316-002)、国家自然科学基金项目(No. 31070100)、安徽建筑工业学院博士基金(No. 2011)和安徽省住房与城乡建设科学技术计划项目(No. 2009-K6-3)资助
更新日期/Last Update: 2012-06-19