|本期目录/Table of Contents|

[1]韩铭,袁月祥,闫志英,等.一株产耐热纤维素酶菌株的筛选及酶学性质[J].应用与环境生物学报,2012,18(01):75-79.[doi:10.3724/SP.J.1145.2012.00075]
 HAN Ming,YUAN Yuexiang,YAN Zhiying,et al.Screening of a Thermostable Cellulase-producing Strain and Its Enzymatic Properties[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):75-79.[doi:10.3724/SP.J.1145.2012.00075]
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一株产耐热纤维素酶菌株的筛选及酶学性质()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年01期
页码:
75-79
栏目:
研究论文
出版日期:
2012-02-25

文章信息/Info

Title:
Screening of a Thermostable Cellulase-producing Strain and Its Enzymatic Properties
作者:
韩铭袁月祥闫志英贺蓉娜刘晓风廖银章
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
Author(s):
HAN Ming YUAN Yuexiang YAN Zhiying HE Rongna LIU Xiaofeng LIAO Yinzhang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
耐热纤维素酶菌株筛选放线菌系统发育分析裂孢菌属酶学性质
Keywords:
thermostable cellulase strain screening actinomycete phylogenetic analysis Thermobifida enzymic property
分类号:
Q939.13+2.06 : Q814.1
DOI:
10.3724/SP.J.1145.2012.00075
文献标志码:
A
摘要:
采用纤维素刚果红平板法从大田蘑菇种植场建堆的稻草样中分离得到了1株能产具有较好温度耐受性和pH稳定性的纤维素酶的放线菌DY3. 综合形态、生理生化特征以及16S rDNA序列分析,将其初步鉴定为嗜热裂孢菌(Thermobifida fusca). 对该菌所产纤维素酶的性质研究表明:最适催化温度为65 ℃,在70 ℃保温60 min后仍有75%以上的活力;最适催化pH为7.5,在pH 5.5~10.0之间该酶的稳定性较好,pH 10.0的条件下仍有80%的活力;最适作用底物是羧甲基纤维素钠. 研究可为木质纤维素的生物预处理提供一定参考价值. 图5 表1 参23
Abstract:
Using CMC Congo Red agar plate method, an actinomycetic strain producing cellulase with high temperature tolerance and pH stability was isolated from the sample collected from mushroom plantation. The strain was identified and named as Thermobifida fusca DY3 by morphological, physiological and biochemical characteristics, and 16S rDNA gene sequence analysis. The study on the cellulase showed that the extracellular thermostable cellualse exhibited the maximum activity at 65 ℃ and retained more than 75% of its maximum activity after incubation for 60 minutes at 70 ℃. The enzyme also showed its pH stability between pH 5.5 and 10.0, and retained 80% of its maximum enzymatic activity after treated at pH 10.0. The sodium carboxymethylcellulose was its best zymolyte. All the characteristics of the enzyme provide reference value for its application in biological pretreatment of lignocellulose. Fig 5, Tab 1, Ref 23

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备注/Memo

备注/Memo:
科技部“863”项目(No. 2007AA100702)和中国科学院重大项目(Nos. KSCXZ-XB23-07-02,KSCXZ-YW-9-008)资助
更新日期/Last Update: 2012-02-29