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[1]许利霞,徐荣,赵焕玉,等.黑胸散白蚁纤维素酶的体外酶学特性[J].应用与环境生物学报,2012,18(01):70-74.[doi:10.3724/SP.J.1145.2012.00070]
 XU Lixia,XU Rong,ZHAO Huanyu,et al.In vitro Characteristics of the Cellulases from Reticulitermes chinensis Snyder[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):70-74.[doi:10.3724/SP.J.1145.2012.00070]
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黑胸散白蚁纤维素酶的体外酶学特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年01期
页码:
70-74
栏目:
研究论文
出版日期:
2012-02-25

文章信息/Info

Title:
In vitro Characteristics of the Cellulases from Reticulitermes chinensis Snyder
作者:
许利霞徐荣赵焕玉杨红
(华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室 武汉 430079)
Author(s):
XU Lixia XU Rong ZHAO HuanyuYANG Hong
(Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China)
关键词:
黑胸散白蚁纤维素酶内切β-14 -葡聚糖酶纤维二糖水解酶β-葡萄糖苷酶酶活Native-PAGE
Keywords:
Reticulitermes chinensis Snyder cellulase endo-β-14-glucanase cellobiohydrolase β-14-glucosidase enzyme activity Native-PAGE
分类号:
Q936 : Q556
DOI:
10.3724/SP.J.1145.2012.00070
文献标志码:
A
摘要:
采用DNS法研究了我国广泛分布的一种低等木食性白蚁——黑胸散白蚁纤维素酶的体外酶活特性以了解其纤维素降解机制. 结果表明,内切β-1,4-葡聚糖酶(EG)、纤维二糖水解酶(CBH)和β-葡萄糖苷酶(BG)这3种酶的最佳反应时间均为15 min,最佳底物浓度为1%,最适反应pH为5.6,最适反应温度为35℃. 在最适反应条件下,EG、CBH和BG的活性分别达到71.3(±13.9) U/mg、5.8(±0.8) U/mg和4.1(±0.7) U/mg. EG在体外的热稳定性较差,在50 ℃及更高温度酶活很低或完全失活,但该酶对pH稳定性较好,在pH 3.2~8.0范围内酶活力变化不大. Native-PAGE电泳检测到该白蚁体内至少有8种不同的EG活性条带,肠道不同部位纤维素酶活性条带种类不同. 这些研究表明,木食性白蚁降解纤维素是一个复杂的过程,需要多种纤维素酶的共同作用. 图8 参17
Abstract:
Reticulitermes chinensis Snyder (Isoptera: Rhinotermitidae) is a wood-feeding lower termite widely distributed in China. For understanding the mechanisms of cellulose degradation in this termite, the in vitro enzyme activities of endo-β-1,4-glucanase (EG), exo-β-1,4-glucanase (namely cellobiohydrolase, CBH), and β-1,4-glucosidase (BG) from the worker termites of R. chinensis were studied with DNS method. The results showed that for the three enzymes, the optimum reaction time, substrate concentration, pH and temperature were 15 min, 1%, 5.6 and 35 ℃, respectively. Under the above conditions, the activities of EG, CBH, and BG was 71.3(±13.9) U/mg, 5.8(±0.8) U/mg and 4.1(±0.7) U/mg, respectively. Endo-β-1,4-glucanase was not stable under high temperature in vitro. The incubation of crude enzyme at above 50 ℃ would result in low activity or inactivation of the enzyme. However, this enzyme was relatively stable in the pH range from 3.2 to 8.0. Native-PAGE electrophoreses showed that there were at least eight different enzyme activity bands corresponding to endo-β-1,4-glucanase distributing in different gut segments in R. chinensis, which indicated the complexity of cellulose degradation process in wood-feeding lower termites. Fig 8, Ref 17

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备注/Memo

备注/Memo:
国家自然科学基金项目(Nos. 30570034,No.30970001)资助
更新日期/Last Update: 2012-02-29