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[1]孙会强,周赛男,谭周进,等.一株烟草内生拮抗放线菌发酵条件优化[J].应用与环境生物学报,2011,17(06):914-917.[doi:10.3724/SP.J.1145.2011.00914]
 SUN Huiqiang,ZHOU Sainan,TAN Zhoujin,et al.Optimization of Fermentation Conditions of an Endophytic Actinomycetes from Tobacco Plant[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):914-917.[doi:10.3724/SP.J.1145.2011.00914]
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一株烟草内生拮抗放线菌发酵条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年06期
页码:
914-917
栏目:
研究简报
出版日期:
2011-12-25

文章信息/Info

Title:
Optimization of Fermentation Conditions of an Endophytic Actinomycetes
from Tobacco Plant
作者:
孙会强周赛男谭周进伍兵谢丙炎申可佳
(1湖南农业大学生物安全科学技术学院 长沙 410128)
(2湖南中医药大学基础医学院 长沙 410208)
(3中国农业科学院蔬菜花卉研究所 北京 100081)
Author(s):
SUN HuiqiangZHOU SainanTAN ZhoujinWU BingXIE BingyanSHEN Kejia
(1School of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128, China)
(2School of Preclinical Medicine, Hunan University of Chinese Medcine, Changsha 410208, China)
(3Institute of Vegetable and Flower, China Academy of Agricultural Sciences, Beijing 100081, China)
关键词:
内生放线菌抑菌作用正交试验发酵优化
Keywords:
endophytic actinomycetes inhibition function orthogonal experiment fermentation condition optimization
分类号:
Q936 : S476.1
DOI:
10.3724/SP.J.1145.2011.00914
文献标志码:
A
摘要:
为提高烟草内生放线菌Y12发酵时产生抑菌物质的产量,通过摇瓶正交优化试验,确定了Y12的三角瓶发酵条件. 发酵培养基组成为:黄豆粉1.5%,蛋白胨1.5%,葡萄糖1.5%,可溶性淀粉1.5%,MgSO4·7H2O 0.05%,(NH4)SO4 0.25%,NaCl 0.4%,KH2PO4 0.1%,CaCO3 0.5%;培养基初始pH 7.5,培养温度30 ℃,发酵时间72 h. 经过优化发酵液的抑菌圈直径增加了44.9%. 图2 表2 参17
Abstract:
In order to increase the yield of the antibacterial substance produced by an endophytic actinomycetes, Y12, from tobacco plant in the process of fermentation, a series of orthogonal experiments on Y12 were carried out in the shaking flasks to obtain optimal components of the fermentation media as well as the fermentation conditions. It was concluded that the optimal proportion of the fermentation medium was: 1.5% soybean flour, 1.5% peptone, 1.5% glucose, 1.5% soluble starch, 0.05% MgSO4·7H2O, 0.25% (NH4)SO4, 0.4% NaCl, 0.1% KH2PO4 and 0.5% CaCO3. The optimal fermentation conditions were: Medium’s initial pH value 7.5, fermentation temperature 30 ℃, and fermentation time 72 hours. It was observed from the experiments that the inhibition diameters of the zymotic fluid increased by 44.9% under the optimized conditions. Fig 2, Tab 2, Ref 17

参考文献/References:

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备注/Memo

备注/Memo:
科技部支撑计划课题(No. 2006BAD08A08)和长沙市科技局课题(No. K1104070-31)资助
更新日期/Last Update: 2011-12-31