|本期目录/Table of Contents|

[1]司静,崔宝凯,贺帅,等.微酸多年卧孔菌产漆酶条件优化及其在染料脱色中的应用[J].应用与环境生物学报,2011,17(05):736-741.[doi:10.3724/SP.J.1145.2011.00736]
 SI Jing,CUI Baokai,He Shuai,et al.Optimization of Conditions for Laccase Production by Perenniporia subacida and Its Application in Dye Decolorization[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):736-741.[doi:10.3724/SP.J.1145.2011.00736]
点击复制

微酸多年卧孔菌产漆酶条件优化及其在染料脱色中的应用()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年05期
页码:
736-741
栏目:
研究论文
出版日期:
2011-10-24

文章信息/Info

Title:
Optimization of Conditions for Laccase Production by Perenniporia subacida and Its Application in Dye Decolorization
作者:
司静崔宝凯贺帅戴玉成
(北京林业大学微生物研究所 北京 100083)
Author(s):
SI JingCUI BaokaiHe ShuaiDai Yucheng
(Institute of Microbiology, Beijing Forestry University, Beijing 100083, China)
关键词:
微酸多年卧孔菌漆酶产酶条件优化染料脱色
Keywords:
Perenniporia subacida laccase optimization of fermentation condition dye decolorization
分类号:
TQ925 + X172
DOI:
10.3724/SP.J.1145.2011.00736
文献标志码:
A
摘要:
微酸多年卧孔菌(Perenniporia subacida)产漆酶能力对生物漂白等研究具有重要意义. 通过单因子和正交试验确定了微酸多年卧孔菌(菌株号:Dai 8224)的最适产酶条件:麦芽糖20 g/L,酵母浸粉5 g/L,pH 5.4,Cu2+ 2.0 mmol/L,培养温度24 ℃,转速160 r/min,接种量1.5%(V/V),此时酶活最高可达1.945 U/mL. 单独使用微酸多年卧孔菌漆酶粗酶液对染料具有很好的脱色效果. 该菌株对于50 mg/L杂环类染料中性红的脱色率可达98.3%,对偶氮染料刚果红的脱色率次之,为91.57%,对亚甲基蓝和铬天青的脱色率也都在80%以上. 此外,其催化中性红脱色的最佳底物浓度为60 mg/L,脱色率达到99.42%,其中,生物降解作用占55.92%,菌体吸附作用占43.5%. 结果表明该菌对多种染料脱色具有较大的应用潜力. 图4 表3 参31
Abstract:
It will be of great significance that the laccase productivity of Perenniporia subacida, a species of white-rot fungi, is explored for bio-blanching. In this study, the optimal culture conditions of strain Dai 8224 for laccase production were obtained by means of single factor analysis and orthogonal test: Maltose 20 g/L, yeast extract 5 g/L, pH 5.4, Cu2+ 2.0 mmol/L, culture temperature 24 ℃, shaking speed 160 r/min, and inoculum 1.5% (V/V), which contributed to the highest enzyme activity as 1.945 U/mL. The crude laccase from P. subacida had the ability to decolor several kinds of dyes including neutral red, congo red, methylene blue and chromazurine. Correspondingly, the decolorization rate was up to 98.3% and 91.57% for neutral red and congo red, respectively, and more than 80% for methylene blue and chromazurine. Furthermore, the optimal concentration of the substrate for decolorizing neutral red was 60 mg/L and the decolorization rate was up to 99.42%, of which 55.91% was biodegraded by laccase and 43.51% was adsorbed by the mycelia. The results indicated that P. subacida (strain Dai 8224) had a great prospect in the treatment of dye-containing wastewater. Fig 4, Tab 3, Ref 31

参考文献/References:

1 Donlagic J, Levec J. Comparison of catalyzed and noncatalyzed oxidation of azo dye and effect on biodegradation. Environ Sci Technol, 1998, 32 (9): 1294~1302
2 Walker GM, Weatherley LR. Biodegradation and biosorption of acid anthraquinone dye. Environ Pollut, 2000, 108 (2): 219~223
3 Dong XL, Zhou JT, Wang J. Decolorization of anthraquinone dye by Rhodopseudomonas XL-1. High Technol Lett, 2002, 8 (2): 11~14
4 Ren SZ, Guo J, Zeng GQ, Sun GP. Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl Microbiol Biotechnol, 2006, 72 (6): 1316~1321
5 Fu CT (傅春堂), Zhang JY (张甲耀), Zheng JX (郑金秀), Zhao L (赵磊), Liu J (柳娟). Isolation and enhanced action of predominant fungi in biological treatment of dying wastewater. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (15): 693~696
6 Wang H (王慧), Zheng XW (郑小伟), Wang BX (王宾香), Xiong XJ (熊小京), Zheng TL (郑天凌). Advances in decolorization of dye by fungi. Chin J Appl Environ Biol (应用与环境生物学报), 2009, 15 (1): 147~151
7 Thurston CF. The structure and function of fungal laccase. Microbiology, 1994, 140: 19~26
8 Xv Y (许颖), Lan J (兰进). Advances in the research of fungal laccase. Acta Edulis Fungi (食用菌学报), 2005, 12 (1): 57~64
9 Have RT, Teunissen PJM. Oxidative mechanisms involved in lignin degradation by white-rot fungi. Chem Rev, 2001, 101: 3397~3413
10 Baldrian P. Purification and characterization laccase from the white-rot fungus Daedalea quercina and decolorization of synthetic dyes by enzyme. Appl Microbiol Biotechnol, 2004, 63 (5): 560~563
11 Wang S (王松), Xie YM (谢益民), Wang P (王鹏), Shuai XH (帅兴华). Removal of lignin and polyphenols of effluent from paper mill by laccase. Environ Sci & Technol (环境科学与技术), 2008, 31 (7): 53~57
12 Ji LC (季立才), Hu PZ (胡培植). Structure, function and application of laccase. Amino Acids Biot Resour (氨基酸和生物资源), 1996, 18 (1): 25~29
13 Langfelder K, Streibel M, Jahn B, Haase G, Brakhage AA. Biosynthesis of fungal melanins and their importance for human pathogenic fungi. Fungal Genet Biol, 2003, 38: 143~158
14 Burke RM, Cairney JW. Laccases and other polyphenol oxidases in ecto- and ericoid mycorrhizal fungi. Mycorrhiza, 2002, 12: 105~116
15 Dai YC. Species diversity of wood-decaying fungi in Northeast China. Mycosystema, 2010, 29 (6): 801~818
16 Dai YC (戴玉成). A checklist of polypores in China. Mycosystema (菌物学报), 2009, 28 (3): 315~327
17 Dai YC (戴玉成), Yang ZL (杨祝良), A revised checklist of medicinal fungi in China. Mycosystema (菌物学报), 2008, 27 (6): 801~824
18 Soares GMB, de Amorim MTP, Costa-Ferreira M. Use of laccase together with redox mediators to decolorize Remazol Brilliant Blue R. J Biotechnol, 2001, 89: 123~129
19 Lin JF (林俊芳), Liu ZM (刘志明), Chen XY (陈晓阳), Guo LQ (郭丽琼), Wang J (王杰). Evaluation of assay methods for determining fungal laccase activity. Chin J Bioproc Engin (生物加工过程), 2009, 7 (4): 1~8
20 Terrón MC, López-Fernández M, Carbajo JM, Junca H, Téllez A, Yagüe S, Arana-Cuenca A, González T, González AE. Tannic acid interferes with the commonly used laccase-detection assay based on ABTS as the substrate. Biochimie, 2004, 86 (8): 519~522
21 Hou HM (侯红漫), Zhou JT (周集体), Wang J (王竞), Lü H (吕红), Du CH (杜翠红), Yan B (严滨). Study of decolorization of anthraquinone dye SN4R by laccase from white-rot fungi Pleurotus ostreatus. J Dalian Univ Technol (大连理工学报), 2004, 44 (5): 640~645
22 Mo JL (莫佳琳), Zhan HY (詹怀宇), Fu SY (付时雨). Identification of a white-rot fungus producing laccase and the application of the laccase in biobleaching. Trans China Pulp & Paper (中国造纸学报), 2008, 23 (2): 10~14
23 Peng D (彭丹), Xie GX (谢更新), Zeng GM (曾光明), Chen YN (陈耀宁), Chen FR (陈芙蓉), Hu S (胡霜), Yu Z (余震). Research and application of producing laccase by Phanerochaete chrysosporium in solid-state fermentation system. Environ Sci (环境科学), 2008, 29 (12): 3568~3573
24 Zhao LG (赵林果), Ji YX (季永新), Li Q (李强), Tang Y (唐莹). Decolorization and degradation of acid violet 43 by immobilized laccase. Ind Microbiol (工业微生物), 2007, 37 (6): 35~40
25 Liu XB (刘晓波), Yan SL (闫世梁), Li ZW (李宗伟), Li PR (李培睿), Li ZY (李宗义). Preliminary study on decolorization of indigo dye and waste water with laccase/HBT mediator system. Environ Poll & Control (环境污染与防治), 2008, 30 (6): 27~30
26 Zhang FM, Knapp JS, Tapley KN. Decolourisation of cotton bleaching effluent with wood rotting fungus. Water Res, 1999, 33 (4): 919~928
27 Nilsson I, Moller A, Mattiasson B, Rubindamayugi MST, Welander U. Decolorization of synthetic and real textile wastewater by the use of white-rot fungi. Enzyme & Microb Technol, 2006, 38: 94~100
28 Wang C, Xi JY, Hu HY, Wen XH. Biodegradation of gaseous chlorobenzene by white-rot fungus Phanerochaete chrysosporium. Biomed & Environ Sci, 2008, 21: 474~478
29 Lu Y, Yan LH, Wang Y, Zhou SF, Fu JJ, Zhang JF. Biodegradation of phenolic compounds from coking wastewater by immobilized white rot fungus Phanerochaete chrysosporium. J Hazard Mater, 2009, 165: 1091~1097
30 Hadibarata T, Tachibana S, Itoh K. Biodegradation of chrysene, an aromatic hydrocarbon by Polyporus sp. S133 in liquid medium. J Hazard Mater, 2009, 164: 911~917
31 Zhang XY (张晓昱), Yan KL (颜克亮), Wang HX (王宏勋). Biodegradation characteristics of triphenylmethane dyes by white rot fungus in rice straw media. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (2): 255~258

相似文献/References:

[1]韩君莉,郭丽琼,郑晓冰,等.灵芝TR6号漆酶的分离纯化及性质研究[J].应用与环境生物学报,2008,14(01):99.
 HAN Junli,et al..Purification and Characterization of Fungal Laccase from Ganoderma lucidum Strain TR6[J].Chinese Journal of Applied & Environmental Biology,2008,14(05):99.
[2]王维乐,牟志美,张淑君,等.响应面法优化Paraconiothyrium variabile GHJ-4产漆酶发酵条件[J].应用与环境生物学报,2011,17(03):321.[doi:10.3724/SP.J.1145.2011.00321]
 WANG Weile,MU Zhimei,ZHANG Shujun,et al.Optimization of Fermentation Conditions of Paraconiothyrium variabile GHJ-4 for Laccase Production by Response Surface Methodology[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):321.[doi:10.3724/SP.J.1145.2011.00321]
[3]刘悦萍,王子健,崔凯,等.一种野生香蘑的分离、鉴定、培养条件与产酶特性[J].应用与环境生物学报,2012,18(05):804.[doi:10.3724/SP.J.1145.2012.00804]
 LIU Yueping,WANG Zijian,CUI Kai,et al.Isolation, Identification, Culture Conditions, and Enzyme Production of a Wild Mushroom Lepista sp.[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):804.[doi:10.3724/SP.J.1145.2012.00804]
[4]秦改娟,马济丰,陈青君,等.一种田头菇属真菌新记录种的生物学与产酶特性[J].应用与环境生物学报,2014,20(01):148.[doi:10.3724/SP.J.1145.2014.00148]
 QIN Gaijuan,MA Jifeng,CHEN Qingjun,et al.Biological characteristics and enzyme production of a newly recorded species of Agrocybe[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):148.[doi:10.3724/SP.J.1145.2014.00148]
[5]罗爽,谢天,刘忠川,等.漆酶/介体系统研究进展[J].应用与环境生物学报,2015,21(06):987.[doi:10.3724/SP.J.1145.2015.09021]
 LUO Shuang,XIE Tian,LIU Zhongchuan,et al.Laccase-mediator system: a review[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):987.[doi:10.3724/SP.J.1145.2015.09021]
[6]夏玉林 冯 娟 李 荷.响应面法优化漆酶基因lac1338表达漆酶的发酵条件[J].应用与环境生物学报,2016,22(02):219.[doi:10.3724/SP.J.1145.2015.06023]
 XIA Yulin,FENG Juan & LI He**.Optimizing fermentation conditions for the expression of laccase gene lac1338by response surface methodology*[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):219.[doi:10.3724/SP.J.1145.2015.06023]
[7]沈柯宇,张西蓓,秦 澎,等.4种重金属对灵芝漆酶活性及转录表达的影响[J].应用与环境生物学报,2017,23(03):448.[doi:2016.08004]
 SHEN Keyu,ZHANG Xibei,QIN Peng,et al.Enzymatic activity and transcription of Ganoderma lucidum laccases following treatment with four heavy metals[J].Chinese Journal of Applied & Environmental Biology,2017,23(05):448.[doi:2016.08004]
[8]胡渤洋,王寿南,陈青君,等.一种白腐真菌的分离、鉴定、培养及产漆酶条件[J].应用与环境生物学报,2018,24(02):367.[doi: 10.19675/j.cnki.1006-687x.2017.05024]
 HU Boyang,WANG Shounan,CHEN Qingjun,et al.Isolation, identification, culture conditions, and laccase production of white rot fungus[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):367.[doi: 10.19675/j.cnki.1006-687x.2017.05024]
[9]秦澎,李津,辜运富,等.香菇生长发育过程中漆酶基因家族的转录表达[J].应用与环境生物学报,2018,24(02):379.[doi: 10.19675/j.cnki.1006-687x.2017.03010]
 QIN Peng,LI Jin,GU Yunfu,et al.Transcriptional expression profiles of the laccase gene family in different development stages of Lentinus edodes[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):379.[doi: 10.19675/j.cnki.1006-687x.2017.03010]
[10]高升,张宇,伍圆圆,等.耐热毛栓孔菌S0301同核体菌株的快速鉴定及产漆酶能力比较[J].应用与环境生物学报,2019,25(01):151.[doi:10.19675/j.cnki.1006-687x.2018.01017]
 GAO Sheng,ZHANG Yu,WU Yuanyuan,et al.A rapid method for the identification of homokaryotic strains in Trametes trogii S0301 and their laccase-producing ability[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):151.[doi:10.19675/j.cnki.1006-687x.2018.01017]

备注/Memo

备注/Memo:
国家自然科学基金项目(No. 30900006)和中央高校基本科研业务费专项资金资助
更新日期/Last Update: 2011-10-25