|本期目录/Table of Contents|

[1]于荣清,刘义,田思琪,等.产油脂微藻的分离鉴定及培养条件优化[J].应用与环境生物学报,2011,17(06):897-900.[doi:10.3724/SP.J.1145.2011.00897]
 YU Rongqing,LIU Yi,TIAN Siqi,et al.Isolation and Identification of Oil Microalgae and Optimization of Its Culture Conditions[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):897-900.[doi:10.3724/SP.J.1145.2011.00897]
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产油脂微藻的分离鉴定及培养条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年06期
页码:
897-900
栏目:
研究论文
出版日期:
2011-12-25

文章信息/Info

Title:
Isolation and Identification of Oil Microalgae and Optimization of Its Culture Conditions
作者:
于荣清刘义田思琪杨云喜李伟曹毅乔代蓉
(四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065)
Author(s):
YU RongqingLIU YiTIAN SiqiYANG YunxiLI WeiCAO YiQIAO Dairong
(Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China)
关键词:
微藻油脂栅列藻属菌株分离鉴定培养条件优化
Keywords:
microalgae lipid Scenedesmus sp. strain isolation and identification optimization of culture condition
分类号:
Q949.206 : TK6
DOI:
10.3724/SP.J.1145.2011.00897
文献标志码:
A
摘要:
为促进自养高产油微藻的工业化应用,从青海多种生境中分离到34株产油微藻,以筛选获得产油脂最高的青3-2-2株为出发株,18S rRNA分析表明其与栅列藻属(Scenedesmus sp.)同源性达到99%以上,确定该株属于栅列藻属. 研究氮源、培养时间、培养温度、初始pH值等培养条件对微藻生物量及油脂含量的影响,优化培养条件为:硝酸钠为氮源,以10%(V/V)接种量接种于普通SE培养基,培养温度为25 ℃,初始pH 7.0,培养周期为20 d,可以获得较高的油脂产率,比优化前的产率增加了近70%. 图3 表1 参16
Abstract:
Thirty-four algae strains were isolated from diverse environments in Qinghai, China. All of them were identified as oil microalgae, and the most oil-rich strain named Qing 3-2-2 was used as the test strain. The homology of 18S rRNA fragments between Qing 3-2-2 and the strains in Scenedesmus genus was high, up to 99% identical. Thus, the strain was identified to belong to Scenedesmus genus. Effects of culture factors, such as N source, incubation time, culture temperature and initial pH value, on biomass production and oil content of algae were also investigated. The optimal culture conditions were: NaNO3 as N source, 10% (V/V) inoculation in general SE medium, culture temperature of 25 ℃, initial pH value of 7.0 and culture period of 20 d. Higher oil productivity was achieved under this optimal culture condition, increasing nearly 70% than before. Fig 3, Tab 1, Ref 16

参考文献/References:

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备注/Memo

备注/Memo:
四川省科技支撑计划(Nos. 2008GZ0021,2008GZ0200)和国家科技基础工作专项重点项目(No. 2008FY11400-4-1)
更新日期/Last Update: 2011-12-31