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[1]王淑智,李利,张道勇,等.NaCl与Cd对小球藻光系统Ⅱ(PSⅡ)活性的影响[J].应用与环境生物学报,2011,17(06):839-846.[doi:10.3724/SP.J.1145.2011.00839]
 WANG Shuzhi,LI Li,ZHANG Daoyong,et al.Effects of NaCl and Cd on PhotosystemⅡ (PSⅡ) Activity of Chlorella pyrenoidosa[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):839-846.[doi:10.3724/SP.J.1145.2011.00839]
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NaCl与Cd对小球藻光系统Ⅱ(PSⅡ)活性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年06期
页码:
839-846
栏目:
研究论文
出版日期:
2011-12-25

文章信息/Info

Title:
Effects of NaCl and Cd on PhotosystemⅡ (PSⅡ) Activity of Chlorella pyrenoidosa
作者:
王淑智李利张道勇潘响亮
(1中国科学院新疆生态与地理研究所环境污染与生态修复实验室 乌鲁木齐 830011)
(2中国科学院地球化学研究所环境地球化学国家重点实验室 贵阳 550002)
(3中国科学院研究生院 北京 100049)
Author(s):
WANG ShuzhiLI LiZHANG DaoyongPAN Xiangliang
(1Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China)
(2State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China)
(3Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
蛋白核小球藻盐胁迫镉胁迫叶绿素荧光光系统Ⅱ(PSⅡ)
Keywords:
Chlorella pyrenoidosa salt stress cadmium stress chlorophyll fluorescence photosystem Ⅱ (PSⅡ)
分类号:
Q945.78
DOI:
10.3724/SP.J.1145.2011.00839
文献标志码:
A
摘要:
运用叶绿素荧光技术分析了盐胁迫和重金属镉(Cd)对蛋白核小球藻(Chlorella pyrenoidosa)光系统Ⅱ(PSⅡ)的影响机理. 实验中小球藻培养基设置NaCl浓度梯度分别为0、25、100 mmol/L,然后在不同NaCl浓度的培养基中再加入CdCl2溶液,使培养基中Cd2+的浓度分别为0、1、25、100 μmol/L. 在处理后的1、3、6、12、24 h测定其快速上升荧光曲线和荧光参数. 实验发现,高浓度的NaCl(≥25 mmol/L)和Cd(≥25 μmol/L)会降低PSⅡ的活性,降低反应中心密度. NaCl主要抑制了依赖于光的反应,降低了光能捕获和用于电子传递的量子产额. Cd主要作用于反应中心,增加了单位反应中心吸收的光能和用于热耗散的能量. 较低浓度(1 μmol/L)的Cd处理对小球藻PSⅡ活性具有促进作用. NaCl和Cd的处理均体现了时间和浓度的依赖性. 盐胁迫和Cd胁迫体现了随浓度增高加重彼此胁迫强度的协同作用的特点. 图7 表1 参28
Abstract:
The effects of NaCl and cadmium (Cd) on photosystem Ⅱ (PSⅡ) activity of Chlorella pyrenoidosa were investigated by chlorophyll fluorescence test to detect the changes of PSⅡ performance caused by environmental stress. NaCl concentrations in media were set as 0, 25 and 100 mmol/L and CdCl2 concentrations in media were 0, 1, 25 and 100 μmol/L. The fluorescence parameters were detected after exposure for 1, 3, 6, 12 and 24 h, respectively. The results showed that the
PSⅡ efficiency and the number of reaction centers decreased under stresses of high levels of NaCl (≥25 mmol/L) and Cd (≥25 μmol/L). The inhibition of NaCl was more related to the light dependent reaction by decreasing the quantum yield for electron transport. The main target site of Cd was the PSⅡ reaction center by increasing the absorbed energy and dissipation in each reaction center. The PSⅡ activity was enhanced by low concentration Cd (1 μmol/L). The effects of NaCl and Cd depended on their concentrations and exposure time, which had synergistically inhibitory effects on PSⅡ activity. Fig 7, Tab 1, Ref 28

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备注/Memo

备注/Memo:
中国科学院知识创新工程重要方向性项目(No. kzcx2-yw-335)、中国科学院“百人计划”项目和国家自然科学基金项目(No. 40673070)资助
更新日期/Last Update: 2011-12-31