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[1]朱习红,刘剑,郑小江,等.麻疯树成熟胚乳再生植株及其气孔分析[J].应用与环境生物学报,2011,17(03):353-358.[doi:10.3724/SP.J.1145.2011.00353]
 ZHU Xihong,LIU Jian,ZHENG Xiaojiang,et al.Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):353-358.[doi:10.3724/SP.J.1145.2011.00353]
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麻疯树成熟胚乳再生植株及其气孔分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年03期
页码:
353-358
栏目:
研究论文
出版日期:
2011-06-24

文章信息/Info

Title:
Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata
作者:
朱习红刘剑郑小江徐莺陈放
(四川大学生命科学学院 成都 610064)
Author(s):
ZHU Xihong LIU Jian ZHENG Xiaojiang XU Ying CHEN Fang
(College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
麻疯树愈伤组织诱导不定芽分化叶片分化生根植株再生气孔分析
Keywords:
Jatropha curcas L. callus induction adventitious bud differentiation leaf differentiation rooting plantlet regeneration stomatal analysis
分类号:
Q943.1 : Q949.753.505
DOI:
10.3724/SP.J.1145.2011.00353
文献标志码:
A
摘要:
对麻疯树成熟胚乳进行组织培养获得胚乳再生植株,并对其气孔进行分析. 麻疯树成熟胚乳在25 ℃、12 h光照条件下培养7 d愈伤组织诱导完成,2,4-D浓度为2.0 mg L-1的MS培养基愈伤诱导效果最好,诱导率达89.29%. 愈伤组织在含BAP的改良培养基上培养至黄绿色后转入分化培养基,在含IAA 0.25 mg L-1和ZT 1.5 mg L-1 的WPM培养基上不定芽分化率达32.50%. 将分化的不定芽从愈伤组织上剥离后转入含IBA、BAP和GA3的培养基上进行芽伸长培养. 取胚乳不定芽叶片接种在含IBA 0.1 mg L-1、BAP 0.5 mg L-1和TDZ 0.5 mg L-1的MS培养基上诱导生芽后,再转入含IAA 0.25mg L-1、KT 0.5mg L-1、BAP 1.0 mg L-1和GA3 0.25 mg L-1的培养基上进行丛生芽的诱导,成芽率为85.2%. 这些芽在含0.1 mg L-1 IBA的1/2 MS培养基上生根,大约有37.5%的芽生了根,平均有5.2条根系形成. 与母本植株相比,再生的胚乳植株保卫细胞更大,且气孔密度减小. 图2 表6 参24
Abstract:
An effective method for endosperm culture of Jatropha curcas L. was established to develop endosperm plantlets and their stomata were analyzed. Vigorously growing calli were obtained from mature endosperm of J. curcas cultured on Murashige and Skoog (MS) medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) at the 7th day under 25 ℃, 12 h light/dark conditions, and a maximum of 89.29% of callus induction frequency was attained. The calli became kelly and had a compact shape when cultured on modified MS medium supplemented with 6-benzyladenine (BAP). Shoot buds were produced when the calli were subcultured on a woody plant medium (WPM) with 1.5 mg L-1 zeatin (ZT) and 0.25 mg L-1 indole-3-acetic acid (IAA), and the rate of shoot differentiation was 32.50%. The regenerated shoots were detached from the calli and transferred to the elongation medium supplemented with indole-3-butyric acid (IBA), BAP and gibberellic acid (GA3). The inoculated leaves from shoot buds regenerated on MS medium supplemented with 0.1 mg L-1 IBA, 0.5 mg L-1 BAP and 0.5 mg L-1 thidiazuron (TDZ). The highest shoot forming rate (85.2%) was obtained on the medium supplemented with 0.25 mg L-1 IAA, 0.5 mg L-1 kinetin (KT), 1.0 mg L-1 BAP and 0.25 mg L-1 GA3. The addition of 0.1 mg L-1 IBA to the 1/2MS medium helped form roots, and approximately 37.5% of the shoots produced five roots. Stomatal analysis showed that the leaves from endosperm-derived plantlets had larger guard cells and lower density of stomata compared with their parent plantlets. Fig 2, Tab 6, Ref 24

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备注/Memo

备注/Memo:
科技部国际合作重点项目(No. 2006DFB63400)和国家“十一五”科技支撑计划课题(No. 2006BAD07A04)
更新日期/Last Update: 2011-06-23