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[1]朱习红,刘剑,郑小江,等.麻疯树成熟胚乳再生植株及其气孔分析[J].应用与环境生物学报,2011,17(03):353-358.[doi:10.3724/SP.J.1145.2011.00353]
　ZHU Xihong,LIU Jian,ZHENG Xiaojiang,et al.Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):353-358.[doi:10.3724/SP.J.1145.2011.00353]
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麻疯树成熟胚乳再生植株及其气孔分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年03期
页码:
353-358
栏目:
研究论文
出版日期:
2011-06-24

文章信息/Info

Title:
Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata
作者:
朱习红; 刘剑; 郑小江; 徐莺; 陈放
（四川大学生命科学学院 成都 610064）
Author(s):
ZHU Xihong;  LIU Jian;  ZHENG Xiaojiang;  XU Ying;  CHEN Fang
(College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
麻疯树; 愈伤组织诱导; 不定芽分化; 叶片分化; 生根; 植株再生; 气孔分析
Keywords:
Jatropha curcas L.;  callus induction;  adventitious bud differentiation;  leaf differentiation;  rooting;  plantlet regeneration;  stomatal analysis
分类号:
Q943.1 : Q949.753.505
DOI:
10.3724/SP.J.1145.2011.00353
文献标志码:
A
摘要:
对麻疯树成熟胚乳进行组织培养获得胚乳再生植株，并对其气孔进行分析. 麻疯树成熟胚乳在25 ℃、12 h光照条件下培养7 d愈伤组织诱导完成，2,4-D浓度为2.0 mg L-1的MS培养基愈伤诱导效果最好，诱导率达89.29%. 愈伤组织在含BAP的改良培养基上培养至黄绿色后转入分化培养基，在含IAA 0.25 mg L-1和ZT 1.5 mg L-1 的WPM培养基上不定芽分化率达32.50%. 将分化的不定芽从愈伤组织上剥离后转入含IBA、BAP和GA3的培养基上进行芽伸长培养. 取胚乳不定芽叶片接种在含IBA 0.1 mg L-1、BAP 0.5 mg L-1和TDZ 0.5 mg L-1的MS培养基上诱导生芽后，再转入含IAA 0.25mg L-1、KT 0.5mg L-1、BAP 1.0 mg L-1和GA3 0.25 mg L-1的培养基上进行丛生芽的诱导，成芽率为85.2%. 这些芽在含0.1 mg L-1 IBA的1/2 MS培养基上生根，大约有37.5%的芽生了根，平均有5.2条根系形成. 与母本植株相比，再生的胚乳植株保卫细胞更大，且气孔密度减小. 图2 表6 参24
Abstract:
An effective method for endosperm culture of Jatropha curcas L. was established to develop endosperm plantlets and their stomata were analyzed. Vigorously growing calli were obtained from mature endosperm of J. curcas cultured on Murashige and Skoog (MS) medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) at the 7th day under 25 ℃, 12 h light/dark conditions, and a maximum of 89.29% of callus induction frequency was attained. The calli became kelly and had a compact shape when cultured on modified MS medium supplemented with 6-benzyladenine (BAP). Shoot buds were produced when the calli were subcultured on a woody plant medium (WPM) with 1.5 mg L-1 zeatin (ZT) and 0.25 mg L-1 indole-3-acetic acid (IAA), and the rate of shoot differentiation was 32.50%. The regenerated shoots were detached from the calli and transferred to the elongation medium supplemented with indole-3-butyric acid (IBA), BAP and gibberellic acid (GA3). The inoculated leaves from shoot buds regenerated on MS medium supplemented with 0.1 mg L-1 IBA, 0.5 mg L-1 BAP and 0.5 mg L-1 thidiazuron (TDZ). The highest shoot forming rate (85.2%) was obtained on the medium supplemented with 0.25 mg L-1 IAA, 0.5 mg L-1 kinetin (KT), 1.0 mg L-1 BAP and 0.25 mg L-1 GA3. The addition of 0.1 mg L-1 IBA to the 1/2MS medium helped form roots, and approximately 37.5% of the shoots produced five roots. Stomatal analysis showed that the leaves from endosperm-derived plantlets had larger guard cells and lower density of stomata compared with their parent plantlets. Fig 2, Tab 6, Ref 24

参考文献/References:

1 Kandpa JB, Madan M. Jatropha curcas: A renewable source of energy for meeting future energy needs. Renewable Energy, 1995, 6 (2): 159~160
2 Openshaw K. A review of Jatropha curcas: an oil plant unfulfilled promise. Biomass Bioenergy, 2000, 19: 1~15
3 Gupta RC. Pharmacognostic studies on dravanti: Jatropha curcas. Plant Sci, 1985, 94 (1): 65~82
4 宋立人. 中华本草 (第四册). 北京: 科学出版社, 1994. 409
5 Thomas TD, Chaturvedi R. Endosperm culture: a novel method for triploid plant Production. Plant Cell Tiss Org Cult, 2008, 93: 1~14
6 Hamada S. Polyploid mulberry tree in practice. Indian J Ser, 1963, 1: 3~4
7 Thomas TD, Bhatnagar AK, Bhojwani SS. Production of triploid plants of mulberry (Morus Alba L.) by endosperm culture. Plant Cell Rep, 2000, 19: 395~399
8 Dimitri L. The resistance of several poplar clonse of the secton leuce to Pollaccia radopsa. Europ J For Path, 1971, 1 (1): 18~32
9 Li Y (李云). Advances in studies of triploid poplar breeding. Chin Bulletin of Botany (植物学通报), 2001, 18 (4): 3~4
10 Zhu DY (朱登云), Li JM (李浚明). The historical and current status of endosperm culture in angiosperms. J Agri Biotechnol (农业生物技术学报), 1996, 4 (3): 205~216
11 Johri BM, Bhojwani SS. Growth responses of mature endosperm in cultures. Nature, 1965, 208: 1345~1347
12 Srivastava PS. In vitro induction of triploid roots and shoots from mature endosperm of Jatropha panduraefolia. Z Pflanzenphysiol, 1971, 66: 93~96
13 Hou P (侯佩), Zhang SW (张淑文), Yang L (杨琳), Xu Y (徐莺), Tang L (唐琳), Wang SH (王胜华), Tan HM (谭慧敏), Chen F (陈放). Callus induction from Jatropha curcas endosperm and elimination of microbial contamination in culture. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (2): 264~268
14 Hou P (侯佩). Studies on endosperm culture of Jatropha curcas L.: [Master Degree Dissertation]. Chengdu, China: Sichuan University (成都: 四川大学), 2006
15 Christopher S. Cramer. Laboratory Techniques for Determining Ploidy in Plants. Hort Technology, 1999, 9 (4): 594~596
16 朱至清. 植物细胞工程. 北京: 化学工业出版社, 2003. 113
17 Straus J, La Rue CD. Maize endosperm tissue grown in vitroⅠ. culture requirements. Am J Bot, 1954, 41: 687~694
18 Li MR, Li HQ, Jiang HW, Pan XP, Wu GJ. Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss Org Cult, 2008, 92: 173~181
19 Johri BM, Srivastava PS. Morphogenesis in endosperm cultures. Z Pflanzenphysiol, 1973, 70: 285~304
20 Ma LZ (马莉贞). Research on browing in plant tissue culture. J Anhui Agri Sci (安徽农业科学), 2006, 34 (15): 3583~3584
21 Carl A, Huetteman, John E, Preece. Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult, 1993, 33: 105~119
22 Dolezˇel J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry. Nature, 2007, 2 (9): 2233~2244
23 Thao NTP, Ureshino K, Miyajima I, Okubo H. Induction of tereaploid in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss Org Cult, 2003, 72: 19~25
24 Masterson J. Stomatal size in fossil plants: Evidence for polyploidy in majority of angiosperms. Science, 1994, 264: 421~423

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备注/Memo

备注/Memo:
科技部国际合作重点项目（No. 2006DFB63400）和国家“十一五”科技支撑计划课题（No. 2006BAD07A04）
更新日期/Last Update: 2011-06-23