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[1]高雅蓉,安君霞,朱启彧,等.银莲花素A对非小细胞肺癌H460的抑制作用[J].应用与环境生物学报,2010,16(05):637-641.[doi:10.3724/SP.J.1145.2010.00637]
 GAO Yarong,AN Junxia,ZHU Qiyu,et al.Inhibitory Effect of Raddeanin A in Human Non-small Cell Lung Cancer H460 Cells[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):637-641.[doi:10.3724/SP.J.1145.2010.00637]
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银莲花素A对非小细胞肺癌H460的抑制作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年05期
页码:
637-641
栏目:
研究论文
出版日期:
2010-10-25

文章信息/Info

Title:
Inhibitory Effect of Raddeanin A in Human Non-small Cell Lung Cancer H460 Cells
文章编号:
201009014
作者:
高雅蓉安君霞朱启彧李甫马超英王明奎唐亚雄
(1西南交通大学生命科学与工程学院 成都 610031)
(2中国科学院成都生物研究所 成都 610041)
Author(s):
GAO YarongAN JunxiaZHU QiyuLI FuMA ChaoyingWANG MingkuiTANG Yaxiong
(1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)
(2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
银莲花素A肺癌细胞H460细胞增殖细胞凋亡细胞周期
Keywords:
Raddeanin A H460 proliferation apoptosis cell cycle
分类号:
R965
DOI:
10.3724/SP.J.1145.2010.00637
文献标志码:
A
摘要:
通过溶剂萃取与重结晶等方法从银莲花属植物多被银莲花Anemone raddeana Regel根茎中提取三萜皂苷银莲花素A,研究银莲花素A对非小细胞肺癌H460癌细胞的增殖、凋亡与细胞周期的影响及分子机制. 结果表明:银莲花素A能显著地抑制H460癌细胞的增殖,且这种抑制效应呈明显的时间量效关系;能诱导H460癌细胞凋亡,并诱导其细胞周期阻滞于G2-M期;能抑制Akt激酶活性、下调Bcl-2蛋白表达以及激活PARP活性,从而导致其对H460癌细胞的增殖抑制作用. 结果显示银莲花素A对非小细胞肺癌的预防与治疗可能具有潜在价值. 图5 参21
Abstract:
Raddeanin A, a triterpenoid saponin, was isolated from Anemone raddeana Regel and identified by solvent extraction and recrystallization. The effect of Raddeanin A on cell proliferation, apoptosis and cell cycle progression of human non-small cell lung cancer (NSCLC) H460 cancer cells and its mechanisms in this aspect were studied in this paper. The results demonstrated that Raddeanin A significantly suppressed proliferation of H460 cancer cells in a dose- and time-dependent manner which was associated with induced apoptosis and cell cycle G2-M arrest. Furthermore, Raddeanin A could inhibit Akt activation, down-regulate the expression of Bcl-2 protein, and activate the cleavage of PARP, leading to marked inhibitory effect of H460 cells. These findings suggest that Raddeanin A may be a potential usefulness for preventing and treating NSCLC. Fig 5, Ref 21

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备注/Memo

备注/Memo:
国家自然科学基金项目(No. 81072662)、四川省青年科技基金(No. 2010JQ0028)、中国科学院“西部之光”人才培养计划重点项目和中国科学院成都生物研究所“人才引进启动项目”资助 Supported by the National Natural Science Foundation of China (No. 81072662), the Youth Science and Technology Foundation of Sichuan, China (No. 2010JQ0028), the West Light Foundation of the Chinese Academy of Sciences (CAS) and the Talent-Recruiting Program of Chengdu Institute of Biology, CAS
更新日期/Last Update: 2010-10-25