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[1]郭娜,李志敏,叶勤.烷烃降解菌的筛选、鉴定及优势菌株的降解特性[J].应用与环境生物学报,2011,17(04):572-576.[doi:10.3724/SP.J.1145.2011.00572]
 GUO Na,LI Zhimin,YE Qin.Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain[J].Chinese Journal of Applied & Environmental Biology,2011,17(04):572-576.[doi:10.3724/SP.J.1145.2011.00572]
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烷烃降解菌的筛选、鉴定及优势菌株的降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年04期
页码:
572-576
栏目:
研究论文
出版日期:
2011-08-25

文章信息/Info

Title:
Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain
作者:
郭娜李志敏叶勤
(华东理工大学生物反应器工程国家重点实验室 上海 200237)
Author(s):
GUO Na LI Zhimin YE Qin
(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China)
关键词:
烷烃降解红球菌菌株筛选16S rDNA石油污染土壤生物修复
Keywords:
alkane-degrading bacterium Rhodococcus strain screen 16S rDNA oil-polluted soil bioremediation
分类号:
X172
DOI:
10.3724/SP.J.1145.2011.00572
文献标志码:
A
摘要:
以正庚烷为唯一碳源,从长期受到石油污染的土壤中筛选获得可利用正庚烷的微生物14株. 通过形态观察和16S rDNA序列比对,鉴定G2、G9、G14为红球菌属,G3、G27为人苍白杆菌属,G4、G7为芽孢杆菌属,G5、G10、G15、G25为节杆菌属,G16为缺陷短波单胞菌,G17、G22为嗜麦芽寡养单胞菌属. 通过考察其降解烷烃的能力,确定Rhodococcus sp. G2为烷烃降解优势菌株. 该菌株可代谢庚烷获得最大菌体浓度D600 nm = 7.51. 同时该菌对不同碳链长度的烷烃,如十二烷、十六烷、煤油和二甲苯均具有较强的降解能力,以十二烷为碳源的最大比生长速率为0.37 h-1,最高菌体浓度为D600 nm = 12.00,在正十六烷中生长,最大比生长速率为0.23 h-1,在煤油中生长,最大比生长速率为0.14 h-1,在以二甲苯为唯一碳源时,D600 nm也可达到1.00左右. 研究表明该菌株对于石油污染土壤的生物修复有很大的应用前景. 图6 表2 参9
Abstract:
Using n-heptane as sole carbon source, 14 strains were enriched and isolated from oil-polluted soil, and their potential in bioremediation was investigated. The results of 16S rDNA sequence analysis and morphological observation showed that G2, G9 and G14 belonged to the genus Rhodococcus; G3 and G27 belonged to Ochrobactrum; G4 and G7 belonged to Bacillus; G5, G10, G15, and G25 belonged to Arthrobacter; G16 belonged to Brevundimonas diminuta; and G17 and G22 belonged to Stenotrophomonas. Among them, Rhodococcus sp. G2 was one of the predominant alkane-degrading strains, which could obtain the maximum D600 nm 7.51 with n-heptane as sole carbon source. In addition, the maximum specific growth rate (μmax) was 0.37 h-1 and D600 nm was 12.00 in n-dodecane, whereas the μmax is 0.23 h-1 in n-hexadecane and 0.14 h-1 in kerosene, respectively. G2 even could utilize xylene to achieve D600 nm 1.00. The results show that G2 can degrade alkane with different carbon chain length including n-heptane, n-dodecane, n-hexadecane, kerosene and xylene, and has great application for bioremediation of oil-polluted soil. Fig 6, Tab 2, Ref 9

参考文献/References:

1 Xu HX (许华夏), Zhang CG (张春桂). Microbial degradation of crude oil contaminated soil. Liaoning Urban & Rural Environ Sci & Technol (辽宁城乡环境科技), 1998, 18 (6): 22~24
2 Li BM (李宝明), Ruan ZY (阮志勇), Jiang RB (姜瑞波). Screen and identification of oil degrading bacteria and community construction. Soil & Fertil Sci China (中国土壤与肥料), 2007,(3): 68~72
3 Atlas RM, Atlas MC. Biodegradation of oil and bioremediation of oil spins . Curr Opin Biotechnol, 1991, 2 (3): 440~443
4 Folsom BR, Schieche DR, Digrazia PM, Werner J, Palmer S. Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-19. Appl Environ Microbiol, 1999, 65 (11): 4967~4972
5 Sorkhoh NA, Ghannoum MA, Ibrahim AS, Stretton RJ, Radwan SS. Crude oil and hydrocarbon degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait. Environ Pollut, 1990, 65 (1): 1~17
6 Song RH (宋若海), Hua ZZ (华兆哲), Chen J (陈坚). Effects of different substrates and environmental factors on hydrocarbon accsess mode and degrading ability of two Pseudomonas aeruginosa strain. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (6): 843~847
7 Saadoun I. Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. J Basic Microbiol, 2002, 42 (6): 420~428
8 Belhaj A, Desnoues N, Elmerich C. Alkane biodegradation in Pseudomonas aeruginosa strains isolated from a polluted zone: Identification of alkB and alkB-related genes.Res Microbiol, 2002, 153 (6): 339~344
9 Franzetti A, Gennaro PD, Bestetti G, Lasagni M, Pitea D, Collina E. Selection of surfactants for enhancing diesel hydrocarbons-contaminated media bioremediation. J Hazardous Mat, 2008, 152 (3): 1309~1316

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备注/Memo

备注/Memo:
发酵工程上海市重点学科项目(No. B505)资助
更新日期/Last Update: 2011-08-16