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[1]陈哈斯额尔敦,余志晟,林文芳,等.一株染料脱色酵母菌的鉴定及脱色特性[J].应用与环境生物学报,2011,17(02):247-250.[doi:10.3724/SP.J.1145.2011.00247]
 CHEN Hasierdun,YU Zhisheng,LIN Wenfang,et al.Identification of a New Isolated Yeast and Its Decolorization on Dyes[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):247-250.[doi:10.3724/SP.J.1145.2011.00247]
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一株染料脱色酵母菌的鉴定及脱色特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年02期
页码:
247-250
栏目:
研究论文
出版日期:
2011-04-25

文章信息/Info

Title:
Identification of a New Isolated Yeast and Its Decolorization on Dyes
作者:
陈哈斯额尔敦 余志晟 林文芳 白志辉 张洪勋
(1中国科学院研究生院资源与环境学院 北京 100049)
(2中国科学院生态环境研究中心 北京 100085)
(3平谷区农村工作委员会 北京 101200)
Author(s):
CHEN Hasierdun YU Zhisheng LIN Wenfang BAI Zhihui ZHANG Hongxun
(1College of Resources and Environment, Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
(2Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
(3Rural Affairs Committee of Pinggu District, Pinggu 101200, Beijing, China)
关键词:
菌种鉴定活性艳红K-2BPPseudozyma rugulosa Y-63脱色废水处理
Keywords:
strain identification Reactive Brilliant Red K-2BP Pseudozyma rugulosa Y-63 decolorization wastewater treatment
分类号:
X172
DOI:
10.3724/SP.J.1145.2011.00247
文献标志码:
A
摘要:
从土壤中分离到一株活性艳红K-2BP脱色酵母菌株Y-63,根据其生理生化特征和26S rRNA基因序列相似性分析,鉴定为Pseudozyma rugulosa. 该菌在16 h内对100 mg/L的活性艳红K-2BP脱色率为94%,其机理属于降解脱色. 该染料脱色的最佳接种量(φ)应不低于5%,最适pH在4~9之间,(NH4)2SO4浓度(w)不低于0.1%,葡萄糖浓度(w)不低于0.2%. 此外,该菌株对其它9种50 mg/L的染料(活性艳蓝X-BR、媒介漂蓝B、活性翠蓝KN-G、酸性媒介黄GG、媒介红S-80、依加仑蓝FBL 200%、弱酸艳红B、活性黑KN-B和活性红M-3BE)的脱色率在10%~96%之间. 该研究表明酵母Y-63在染料废水的处理上具有较好的应用潜能. 图2 表2 参14
Abstract:
A new isolated yeast Y-63, capable of degrading Reactive Brilliant Red K-2BP, was obtained from soil, and identified belonging to Pseudozyma rugulosa by its physio-biochemical characteristics and similarity analysis of 26S rRNA gene sequence. This strain gave a maximal decolorization (94%) for Reactive Brilliant Red K-2BP (100 mg/L) after incubation for 16 h. To get this decolorizing effect, the optimal inoculation volume (φ) of Y-63 was set not less than 5%, optimal pH of culture medium ranging from 4 to 9, and concentrations of (NH4)2SO4 and glucose not lower than 0.1% and 0.2%, respectively. In addition, it could also decolorize 10%~96% of other 9 dyes (Reactive Brilliant Blue X-BR, Mordant Blue B, Reactive Turquoise Blue KN-G, Acid Mordant Yellow GG, Acid Mordant Red S-80, Acid Blue FBL 200%, Weak Acid Brilliant Red B, Reactive Black KN-B and Acid Red M-3BE) of 50 mg/L. These results indicated that yeast Y-63 would have potential application in dye wastewater treatment. Fig 2, Tab 2, Ref 14

参考文献/References:

1 Xu WC (胥维昌). The current situation and prospect of wastewater-treatment in dyestuff industry. Dyestuff Ind (染料工业), 2002, 39 (6): 35~39 
2 Dai SG (戴树桂), Song WH (宋文华), Li T (李彤), Zhuang YY (庄源益). The study of quantitative structure - biodegradability relationships for azo dyes. Adv Environ Sci (环境科学进展), 1996, 4 (6): 56~60
3 Mei H (梅嬛), Hong Q (洪青), Li SP (李顺鹏). Isolation, identification and characterization of a malachite green-degrading bacterium. Chin J Appl Environ Biol (应用与环境生物学报), 2010, 16 (3): 390~393
4 Banat IM, Nigam P, Singh D. Microbial decolorization of textile-dye-containing effluents: A review. Bioresour Technol, 1996, 58: 217~227
5 Fu Y, Viraraghavan T. Fungal decolorization of dye wastewaters: A review. Bioresour Technol, 2001, 79: 251~262
6 Itoh K, Kitade Y, Yatome C. A pathway for biodegradation of an anthraquinone dye, C. I. Disperse Red 15, by a yeast strain Pichia anomala. J Biotechnol, 1998, 53: 83~89
7 Ramalho PA, Cardoso MH, Cavaco-Paulo A, Ramalho MT. Characterization of azo reduction activity in a novel ascomycete yeast strain. Appl &Environ Microbiol, 2004, 70 (4): 2279~2288
8 Martins MAM, Cardoso MH, Queiroz MJ, Ramalho MT, Campos AMO. Biodegradation of azo dyes by the yeast Candida zeylanoides in batch aerated cultures. Chemosphere, 1999, 38 (11): 2455~2460
9 Yang Q, Yang M, Pritsch K, Yediler A, Hagn A, Schloter M, Kettrup A. Decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates. Biotechnol Lett, 2003, 25: 709~213
10 Yang Q, Yediler A, Yang M, Kettrup A. Decolorization of an azo dye, Reactive Black 5 and MnP production by yeast isolate: Debaryomyces polymorphus. Biochem Engin J, 2005, 24: 249~253
11 Lucas MS, Amaral C, Sampaio A, Peres JA, Dias AA. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme & Microb Technol, 2006, 39: 51~55
12 Jadhav JP, Parshetti GK, Kalme SD, Govindwar SP. Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463. Chemosphere, 2007, 68: 394~400
13 Lucas MS, Dias AA, Sampaio A, Amaral C, Peres JA. Degradation of a textile reactive azo dye by a combined chemical–biological process: Fenton’s reagent-yeast. Water Res, 2007, 41: 1103~1109
14 Barne RJA, Payne R, Yarrow D. Yeasts: Characteristics and Identification. New York, USA: Cambridge University Press, 1983. 13~59, 336

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备注/Memo

备注/Memo:
国家自然科学基金项目(No. 20877098)、中国科学院研究生院院长基金项目(No. M3018)和北京市科学技术委员会科技新星项目(No. 2006A78)资助
更新日期/Last Update: 2011-04-25