|本期目录/Table of Contents|

[1]赵高梅,龙海,邓光兵,等.青稞B-hordein基因表达载体构建及对小麦的遗传转化[J].应用与环境生物学报,2011,17(03):364-368.[doi:10.3724/SP.J.1145.2011.00364]
 ZHAO Gaomei,LONG Hai,DENG Guangbing,et al.Vector Construction and Genetic Transformation of B-hordein Gene from Hulless Barley to Wheat[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):364-368.[doi:10.3724/SP.J.1145.2011.00364]
点击复制

青稞B-hordein基因表达载体构建及对小麦的遗传转化()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年03期
页码:
364-368
栏目:
研究论文
出版日期:
2011-06-24

文章信息/Info

Title:
Vector Construction and Genetic Transformation of B-hordein Gene from Hulless Barley to Wheat
作者:
赵高梅龙海邓光兵潘志芬余懋群
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
Author(s):
ZHAO Gaomei1 LONG Hai DENG Guangbing PAN Zhifen YU Maoqun
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
青稞B组醇溶蛋白载体构建转基因小麦GATEWAY?
Keywords:
hulless barely B-hordein vector construction gene transformation wheat GATEWAY?
分类号:
S512.103
DOI:
10.3724/SP.J.1145.2011.00364
文献标志码:
A
摘要:
利用从具有特殊B-hordein亚基组成的青藏高原青稞材料中克隆的B-hordein编码基因(SL60)和小麦高分子量谷蛋白亚基胚乳特异表达启动子PGlu1Dx构建了真核表达载体pCB2007-SL60. 通过农杆菌介导对普通小麦进行遗传转化,共获得227株再生植株,经PCR鉴定和转化片段测序验证,最终获得5株阳性植株. 研究为进一步分析B-hordein基因在小麦背景中的遗传和表达,以及对小麦加工特性的影响奠定了基础. 图5 表2 参21
Abstract:
By the GATEWAY? strategy, a eukaryotic expression vector (pCB2007-SL60) was constructed to express a B-hordein gene isolated from hulless barley in the Qinghai-Tibetan Plateau, China. It was driven by an endosperm-specific promoter of high-moleculer-weight glutenin subunit of wheat and was transformed by Agrobacterium-mediated method in wheat. Totally, 227 transgenic plants were obtained, and of them 5 positive plants were screened by means of PCR amplification. It was a key step to study the expression and heredity of B-hordein gene in wheat, and its potential effect on the wheat quality properties. Fig 5, Tab 2, Ref 21

参考文献/References:

1 Rahman S, Kreis M, Forde BG, Shewry PR, Miflin BJ. Hordein-gene expression during development of the barley (Hordeum vulgare) endosperm. J Biol Chem, 1984, 223 (2): 315~322
2 Peltonen J, Rita H, Aikasalo R, Home S. Hordein and malting quality in northern barleys. Hereditas, 1994, 120 (3): 231~239
3 Lehfer H, Busch W, Martin R, Herrmann RG. Localization of the B-hordein locus on barley chromosomes using fluorescence in situ hybridization. Chromosoma, 1993, 102 (6): 428~432
4 Brandt A, Montembault A, Cameron-Mills V, Rasmussen SK. Primary structure of A B1 hordein gene from barley. Carlsberg Res Commun, 1985, 50 (6): 333~345
5 Forde BG, Heyworth A, Pywell J, Kreis M. Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize. Nucleic Acids Res, 1985, 13 (20): 7327~7339
6 Vicente-Carbajosa J, Beritashvili DR, Kraev AS, Skryabin KG. Conserved structure and organization of B hordein genes in the Hor 2 locus of barley. Plant Mol Biol, 1992, 18 (3): 453~458
7 Hou YC, Liu Q, Long H, Wei YM, Zheng YL. Characterization of low-molecular-weight glutenin subunit genes from Hordeum brevisubulatum ssp. Turkestanicum. Biol Bull, 2006, 33 (1): 35~42
8 Piston F, Martin A, Dorado G, Barro F. Cloning and molecular characterization of B-hordeins from Hordeum chilense (Roem. et Schult.). Theor Appl Genet, 2005, 111 (3): 551~560.
9 Han ZX, Qian G, Pan ZF, Deng GB, Wu F, Tang YW, Qiang XL, Yu MQ. Cloning and characterization of four B-hordein genes from Tibetan hull-less Barley (Hordeum vulgare subsp. vulgare). Acta Genet Sin, 2006, 33 (10): 937~947
10 Lamacchia1 C, Shewry PR, Natale Di Fonzo, Forsyth JL, Nick Harris, Lazzeri1 PA, Napier JA, Halford NG, Barcelo P. Endosperm-specific activity of a storage protein gene promoter in transgenic wheat seed. J Exp Bot, 2001, 52 (335): 243~250
11 Lei ZY, Zhao P, Cao MJ, Cui R, Chen X, Xiong LZ, Zhang QF, Oliver D J, Xiang CB. High-throughput binary vectors for plant gene function. J Integr Plant Biol, 2007, 49 (4): 556~567
12 Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol, 1997, 115 (3): 971~980
13 Mtuashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 1962, 15: 473~497
14 Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allrad RW. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Nat Acad Sci USA, 1984, 81 (24): 8014~8018
15 Han ZX, Qian G, Wu F, Pan ZF, Deng GB, Yu MQ. Sequences variation and classi?cation of B-hordein genes in hull-less barley from Qinghai–Tibet Plateau. Mol Biol, 2008, 42 (1): 56~63
16 Han ZX, Wu F, Deng GB, Qian G, Yu MQ, Jia YL. Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley. Genetica, 2010, 138 (2): 227~239
17 Payne PI. Genetics of wheat storage protein and the effect of allelic variation on bread-making quality. Ann Rev Plant Physiol, 1987, 38: 141~153
18 Gupta RB, Macritchie F. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3, and Gli-1 of common wheat. II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci, 1994, 19 (1): 19~29
19 Gupta RB, Paul JG, Cornish GB, Palmer GA, Bekes F, Rathjen AJ. Allelic variation at glutenin subunit and gliadin loci, Glu-1,Glu-3,and Gli-1, of common wheats. I. Its additive and interaction effects on dough properties. J Cereal Sci, 1994, 19 (1): 9~17
20 Li L (李林), Liu L (刘琳), Yi L (易玲), Xu DL (徐德林), Long H (龙海), Pan ZF (潘志芬), Deng GB (邓光兵), Yu MQ (余懋群), Peng ZS (彭正松). The callus induction of immature embryo and plant regeneration of seven spring wheat varieties in Heilongjiang. Mod Agric Sci (现代农业科学), 2009, 16 (3): 10~12
21 Pan ZF (潘志芬), Tang YW (唐亚伟), Wu F (吴芳), Han ZX (韩兆雪), Deng GB (邓光兵), Yu MQ (余懋群). Genetic diversity of B hordein in hulless barley (Hordeum vulgare L.) from the Qinghai-Tibet Plateau in China. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (5): 601~604

相似文献/References:

[1]赵桃,刘英华,邓光兵,等.青稞穗发芽抗性测定方法的评价[J].应用与环境生物学报,2009,15(03):380.[doi:10.3724/SP.J.1145.2009.00380]
 ZHAO Tao,LIU Yinghua,DENG Guangbing,et al.Assessment of Methods Used in Testing Preharvest Sprouting Resistance in Hulless Barley[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):380.[doi:10.3724/SP.J.1145.2009.00380]
[2]唐珊珊,顾楠,潘志芬,等.青稞发芽过程中β-葡聚糖含量变化及对麦芽品质的影响[J].应用与环境生物学报,2015,21(04):616.[doi:10.3724/SP.J.1145.2015.02012]
 TANG Shanshan,GU Nan,PAN Zhifen,et al.Changes in β-glucan content of hull-less barley during germination and the effects on malt quality[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):616.[doi:10.3724/SP.J.1145.2015.02012]
[3]翟会生,唐珊珊,潘志芬,等.青稞籽粒主要组分对其淀粉膨胀势的影响[J].应用与环境生物学报,2017,23(2):193.[doi:10.3724/SP.J.1145.2016.03009]
 ZHAI Huisheng,TANG Shanshan,et al.Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):193.[doi:10.3724/SP.J.1145.2016.03009]

备注/Memo

备注/Memo:
国家转基因生物新品种培育科技重大专项(No. 2008ZX08009-003)和国家科技基础性工作专项(No. 2006FY110700)资助
更新日期/Last Update: 2011-06-23